Standard name
Description Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.18 0.3 0.38 0.25 0.2 0.27 0.26 0.23 0.21 0.26 0.26 0.27 0.27 0.3 0.28 0.24 0.21 0.21 0.09 0.13 0.17 0.16 0.15 0.17
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0.15 0.06 0 0.06 0.1 0 0 0 0 0 0 0 0.11 0.14 0.12 0.19 0.29 0.25 0.07 0.06 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.25 0.36 0.4 0.25 0.51 0.49 0.54 0.69 0.72 0.7 0.74 0.11 0.06 0.27 0.05 0 0.07 0 0 0.08 0 0 0.05
Nucleus 0.32 0.21 0.14 0.11 0.06 0.1 0.12 0.14 0.12 0.12 0.1 0.08 0.36 0.22 0.11 0.22 0.22 0.23 0.27 0.23 0.17 0.39 0.34 0.24
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0.06 0 0 0.06 0.05 0 0 0.05 0 0 0 0.13 0.07 0.14 0.1 0.14 0.13 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.21 0.11 0.16 0.25 0.45 0.21 0.21 0.2 0.13 0.09 0.08 0.07 0.1 0.22 0.15 0.26 0.27 0.23 0.26 0.24 0.23 0.07 0.07 0.08
Vac/Vac Membrane 0.18 0.18 0 0 0.06 0.08 0.07 0.05 0.05 0 0.05 0.06 0.13 0.16 0.1 0.19 0.15 0.19 0.16 0.18 0.21 0.27 0.27 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0
Bud 1 0 4 13 13 6 15 31 18 20 18 17 0 3 7 1 0 1 6 5 5 3 8 5
Bud Neck 0 0 6 2 12 7 11 7 2 6 6 2 0 1 0 0 0 0 1 2 0 1 1 4
Bud Site 0 1 1 2 4 5 11 20 8 18 8 16 0 1 0 0 1 0
Cell Periphery 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 55 71 94 63 64 105 134 119 97 144 96 144 26 49 50 83 72 76 34 42 56 28 29 40
Endoplasmic Reticulum 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 19 16 18 0 0 2 0 1 0
Endosome 46 15 5 16 33 7 11 16 2 1 1 3 11 22 21 67 101 90 29 18 13 1 4 6
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 4 4 0 0 2 0
Mitochondria 9 60 91 104 83 197 252 286 316 392 259 399 11 10 48 19 13 24 14 12 25 5 3 13
Nucleus 97 50 36 29 20 39 60 74 54 64 36 44 35 35 20 75 76 84 108 72 57 70 67 58
Nuclear Periphery 0 1 1 0 0 0 0 2 0 1 1 1 0 0 1 0 0 2 4 2 1 0 0 0
Nucleolus 27 13 7 6 21 21 11 17 22 18 7 9 13 12 25 34 49 46 17 8 6 3 5 6
Peroxisomes 0 0 0 4 16 2 2 4 0 2 0 2 0 8 7 0 2 4 0 0 0 0 0 0
SpindlePole 64 26 41 65 149 80 108 104 60 50 28 39 10 36 26 90 91 86 104 74 80 11 14 20
Vac/Vac Membrane 54 43 7 11 21 32 38 26 25 18 18 30 13 26 18 66 52 68 64 56 73 47 54 75
Unique Cell Count 303 236 250 257 328 389 512 525 461 547 372 536 97 161 177 348 343 367 402 313 344 179 199 240
Labelled Cell Count 357 280 293 315 436 501 654 707 604 734 478 706 120 203 223 455 474 499 402 313 344 179 199 240


Cytoplasm, Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 3.8 3.4 3.6 3.6 3.4 3.1 3.3 2.7 2.6 2.8 2.5 4.2 4.5 4.0 5.9 6.3 6.5 4.3 4.4 4.7
Std Deviation (1e-4) 0.8 0.8 0.6 1.2 1.3 1.3 1.1 1.4 1.0 0.9 1.1 0.9 1.2 1.1 1.2 1.3 1.8 1.8 0.7 1.4 1.1
Intensity Change (Log2) 0.08 0.08 0.0 -0.11 -0.03 -0.31 -0.38 -0.27 -0.45 0.34 0.42 0.23 0.81 0.9 0.94 0.36 0.38 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 6.0 5.0 5.9


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available