Standard name
Description Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.06 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0.09 0 0 0 0 0 0
Cytoplasm 0.93 0.94 0.94 0.93 0.92 0.86 0.8 0.78 0.74 0.74 0.63 0.64 0.99 0.99 0.98 0.9 0.94 0.94 0.84 0.86 0.81 0.89 0.91 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.1 0.1 0.35 0.39 0.36 0.37 0.45 0.55 0.53 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 2 0 2 1 1 0 0 0 0 0 0 0 0 1 2 1 0 3 3 3 1 0 1
Bud 6 2 3 5 3 2 17 8 22 31 18 14 0 0 1 0 0 1 6 2 8 3 2 3
Bud Neck 0 2 0 0 2 0 2 3 1 3 2 0 0 2 3 1 0 0 9 5 9 5 2 1
Bud Site 2 0 2 0 1 3 1 2 2 3 1 3 0 0 0 2 0 0 – – – – – –
Cell Periphery 6 4 18 16 4 8 8 8 15 12 9 7 5 12 4 11 9 10 2 0 1 0 0 0
Cytoplasm 211 184 297 244 274 296 379 394 293 332 179 225 170 386 293 129 90 101 197 171 258 218 228 222
Endoplasmic Reticulum 0 0 1 0 0 1 0 0 0 0 1 2 1 1 0 11 5 5 0 0 5 0 0 0
Endosome 1 3 1 4 6 1 4 7 1 2 1 1 0 2 0 1 0 0 3 1 3 2 0 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 1 2 0 0 0
Mitochondria 14 2 7 26 30 121 185 180 147 205 156 189 0 1 0 5 2 1 2 1 10 2 3 1
Nucleus 1 1 2 0 1 1 3 3 2 3 4 3 0 2 0 3 0 2 0 0 0 1 0 1
Nuclear Periphery 0 0 0 0 1 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 2 3 1 2 2 1 0 4 0 0 0 0 2 0 0 0 0 1 0 0 0
Peroxisomes 2 0 2 0 1 3 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 1 11 3 3 7 7 3 6 7 2 7 0 0 1 3 1 2 4 8 7 4 0 2
Vac/Vac Membrane 0 3 2 2 5 1 8 11 14 7 3 5 0 0 2 3 4 3 1 0 1 1 5 1
Unique Cell Count 226 195 317 261 297 346 476 504 395 451 282 354 171 390 298 143 96 107 234 198 319 246 251 246
Labelled Cell Count 248 204 346 304 335 446 617 621 504 606 380 459 176 406 306 173 113 125 234 198 319 246 251 246


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 6.5 5.5 4.9 5.3 4.2 4.2 4.3 4.2 3.8 3.5 3.7 6.6 6.3 6.3 7.6 7.1 7.3 6.7 7.1 7.6
Std Deviation (1e-4) 0.8 1.4 1.4 1.2 2.3 1.3 1.7 2.0 3.1 1.7 1.3 1.4 1.3 0.9 1.0 1.9 1.7 2.0 1.1 1.4 1.7
Intensity Change (Log2) – – – -0.16 -0.05 -0.4 -0.39 -0.36 -0.39 -0.52 -0.65 -0.58 0.26 0.19 0.19 0.45 0.37 0.41 0.28 0.36 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.2 -2.9 0 0 0 0 0 0 0 -1.4 -1.7 -2.9 0.8 1.3 1.3
Cytoplasm -0.1 -0.7 -3.4 -5.5 -5.9 -6.9 -7.1 -9.1 -9.4 3.0 3.9 2.9 -1.3 0 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.0 4.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -1.8 -2.0 0 0 0 0 0 0 0 0 0 -2.8 0 0 0
Vacuole 0 0 0 0 1.7 2.6 0 0 0 0 0 0 0 0 0