Standard name
Human Ortholog
Description Subunit of NuA4 histone H4 acetyltransferase and SWR1 complexes; may function to antagonize silencing near telomeres; interacts directly with Swc4p; has homology to human leukemogenic protein AF9; contains a YEATS domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.25 0.53 0.59 0.67 0.77 0.65 0.73 – 0.53 0.63 0.51 0.56 0.42 0.65 0.69 0.22 0.19 0.12 0.15 0.34 0.26 0.27 0.31 0.29
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.08 0 0 0 0 0 – 0 0 0 0 0.07 0 0.05 0 0 0 0.06 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.56 0.12 0 0 0 0.24 0.09 – 0.4 0.26 0.47 0.38 0.05 0 0 0.05 0 0 0.15 0.07 0.12 0.09 0.07 0
Nucleus 0.25 0.18 0.32 0.25 0.14 0.18 0.15 – 0.17 0.18 0.21 0.16 0.35 0.26 0.15 0.72 0.81 0.76 0.23 0.24 0.21 0.24 0.2 0.17
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0.12 0 0 0 0.06 0.11 0.09
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.09 0.07 0.07 0.1
Vac/Vac Membrane 0.12 0.21 0.06 0 0 0 0 – 0 0 0 0 0.17 0.05 0.15 0 0 0.06 0.19 0.17 0.16 0.13 0.15 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Bud 0 0 0 0 2 0 0 – 3 2 5 0 0 0 0 0 0 0 0 3 0 1 2 2
Bud Neck 0 0 1 1 5 5 5 – 3 7 4 4 0 0 0 0 0 0 0 0 0 1 1 1
Bud Site 0 0 0 0 0 1 1 – 1 0 12 4 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 59 40 149 266 150 228 – 124 153 140 176 17 92 119 13 5 2 10 48 28 22 44 43
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 2 0 0 0 0 2 1 0 2
Endosome 0 9 1 6 8 4 7 – 1 4 1 2 3 6 8 0 0 0 4 3 3 1 4 7
Golgi 0 0 0 0 0 1 0 – 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 9 13 0 8 6 56 28 – 92 62 129 120 2 4 1 3 0 0 10 10 12 7 10 6
Nucleus 4 20 22 55 49 42 47 – 40 43 59 50 14 37 26 42 21 13 16 34 22 20 28 24
Nuclear Periphery 0 0 0 0 0 0 0 – 1 1 2 3 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2 1 0 0 – 0 1 1 4 0 1 0 4 0 2 2 3 4 5 15 13
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 5 9 14 1 12 – 3 3 11 8 0 3 3 0 0 0 5 8 9 5 10 15
Vac/Vac Membrane 2 23 4 3 13 6 13 – 10 4 6 3 7 7 26 0 0 1 12 24 17 10 20 25
Unique Cell Count 16 112 68 222 347 232 313 232 243 275 315 40 141 173 58 26 17 70 144 109 83 142 149
Labelled Cell Count 19 124 73 233 364 266 341 280 280 370 374 43 150 185 66 26 18 70 144 109 83 142 149


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.4 4.9 4.7 5.0 3.8 4.4 – 3.8 3.8 3.7 3.6 4.6 4.9 4.9 8.5 8.8 8.3 4.7 4.5 4.7
Std Deviation (1e-4) 0.3 1.1 1.0 1.6 1.4 1.6 1.8 – 1.6 1.2 1.4 0.8 1.3 1.2 1.4 2.1 2.2 3.0 2.3 1.7 2.0
Intensity Change (Log2) – – – -0.05 0.04 -0.36 -0.17 – -0.37 -0.37 -0.42 -0.45 -0.1 0.0 -0.01 0.79 0.84 0.75 -0.07 -0.13 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.1381 -1.09 -1.2717 -1.1361 -1.1671 -1.0701 0.945 0.2832 0.0129 -0.2183 0.2672 0.6545 2.138 1.7776 1.3921 1.6035 1.8333 1.695
Actin 0.0197 0.0039 0.0045 0.0017 0.0081 0.0041 0.0072 0.0039 0.0042 0.0247 0.0006 0.0057 0.0431 0.0005 0.0068 0.0216 0.0007 0.0064
Bud 0.0004 0.0001 0.0001 0.0001 0.0011 0.0003 0.0016 0.0189 0.0019 0.0008 0.0002 0.0053 0.0023 0.0018 0.0008 0.0016 0 0.001
Bud Neck 0.0007 0.0023 0.0005 0.0002 0.011 0.0034 0.0308 0.0043 0.0009 0.0007 0.003 0.0453 0.0016 0.0005 0.0018 0.0016 0.0002 0.0034
Bud Periphery 0.0005 0.0001 0.0001 0.0001 0.0013 0.0004 0.0021 0.0085 0.0016 0.0023 0.0002 0.0031 0.0038 0.0017 0.0009 0.0014 0 0.0019
Bud Site 0.0018 0.0088 0.0007 0.0004 0.0254 0.0004 0.0137 0.0113 0.0126 0.0007 0.0004 0.0011 0.0044 0.0018 0.003 0.0051 0.0001 0.0012
Cell Periphery 0.0004 0.0002 0.0001 0 0.0008 0.0001 0.0009 0.0004 0.0002 0.0001 0 0.0002 0.0005 0.0002 0.0003 0.0002 0 0.0002
Cytoplasm 0.0958 0.0886 0.0805 0.1537 0.0807 0.2161 0.0631 0.1802 0.1544 0.183 0.1748 0.128 0.1277 0.1946 0.126 0.0989 0.1279 0.1386
Cytoplasmic Foci 0.0335 0.0367 0.0392 0.0995 0.0095 0.0161 0.103 0.0172 0.0152 0.011 0.0024 0.025 0.0303 0.0064 0.0076 0.0173 0.0174 0.0068
Eisosomes 0.0053 0.0003 0.0002 0 0.0015 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0115 0.0028 0.0044 0.0022 0.0075 0.0113 0.0033 0.0048 0.0038 0.0032 0.0024 0.0035 0.0039 0.0035 0.0037 0.0024 0.0036 0.0019
Endosome 0.0637 0.0143 0.0118 0.0473 0.0907 0.0329 0.0721 0.0054 0.0177 0.0095 0.0075 0.0309 0.0325 0.0045 0.0083 0.0229 0.0502 0.0114
Golgi 0.0371 0.0027 0.0053 0.0129 0.0023 0.0061 0.0247 0.0008 0.0081 0.0053 0.0002 0.0185 0.0149 0.0002 0.0026 0.0012 0.0015 0.0071
Lipid Particles 0.0405 0.0102 0.0296 0.0186 0.0033 0.0258 0.0395 0.0015 0.0074 0.0037 0.0002 0.008 0.0371 0.0003 0.0023 0.0017 0.0194 0.006
Mitochondria 0.0075 0.002 0.0116 0.0013 0.0054 0.003 0.0113 0.0085 0.006 0.037 0.002 0.0146 0.0123 0.001 0.0019 0.005 0.0005 0.0076
None 0.2721 0.4711 0.3939 0.4421 0.3436 0.2086 0.0046 0.4572 0.3935 0.2919 0.0311 0.1549 0.15 0.4681 0.4426 0.461 0.5494 0.2726
Nuclear Periphery 0.0184 0.0231 0.0314 0.0012 0.0226 0.0443 0.0052 0.0123 0.008 0.01 0.0833 0.0078 0.0105 0.0079 0.0106 0.014 0.0082 0.0076
Nucleolus 0.0121 0.0034 0.0031 0.0034 0.0208 0.0139 0.018 0.006 0.0037 0.0035 0.0123 0.0117 0.0055 0.0021 0.0057 0.0129 0.0009 0.0084
Nucleus 0.3356 0.2656 0.3144 0.1653 0.2249 0.3685 0.5057 0.207 0.3102 0.3409 0.5361 0.4719 0.4175 0.2421 0.335 0.2803 0.1745 0.4586
Peroxisomes 0.0141 0.0214 0.0268 0.0425 0.0015 0.005 0.0312 0.0014 0.0074 0.0035 0.0001 0.002 0.0128 0.0002 0.0011 0.0047 0.0009 0.0103
Punctate Nuclear 0.0191 0.0398 0.0376 0.0056 0.0593 0.0322 0.0477 0.0469 0.0397 0.0635 0.1348 0.0591 0.0802 0.0553 0.0269 0.0376 0.031 0.0312
Vacuole 0.0076 0.0021 0.0032 0.0014 0.0529 0.0041 0.0118 0.0027 0.0027 0.0033 0.003 0.0026 0.0071 0.0068 0.0111 0.0062 0.0121 0.0156
Vacuole Periphery 0.0024 0.0004 0.0011 0.0006 0.0258 0.0033 0.0021 0.0008 0.0007 0.0014 0.0054 0.0008 0.0014 0.0006 0.0009 0.0022 0.0013 0.0022

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.9622 7.2421 5.374 13.3194 15.7556 9.7162 14.645 11.8613 15.4787 18.3482
Translational Efficiency 1.8372 2.5396 2.8287 1.0586 1.2238 1.7992 1.3556 1.3943 1.1722 1.3389

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of NuA4 histone H4 acetyltransferase and SWR1 complexes; may function to antagonize silencing near telomeres; interacts directly with Swc4p; has homology to human leukemogenic protein AF9; contains a YEATS domain
Localization
Cell Percentages nucleus (35%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Yaf9

Yaf9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yaf9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available