Standard name
Description Component of the Rpd3L histone deacetylase complex; involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus shares significant sequence identity with the human candidate tumor suppressor p33-ING1 and its isoform ING3

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.06 0 0 0 0 0 0.05 0 0.09 0.16 0.18 0.17 0.16 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.08 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.05 0.06 0 0.09 0.43 0.28 0.28 0.26 0.25 0.41 0.17 0 0 0 0.07 0 0 0 0 0 0 0 0
Nucleus 0.93 0.92 0.89 0.92 0.86 0.75 0.74 0.73 0.73 0.73 0.63 0.68 0.89 0.82 0.81 0.65 0.62 0.59 0.86 0.83 0.71 0.66 0.66 0.57
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 0.14 0 0.07 0.2 0.28 0.28 0.33 0.32 0.3 0.39 0.1 0 0 0 0 0 0.06 0.07 0.18 0.16 0.14 0.18
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.19 0.14 0.05 0 0 0.07 0.08 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 1
Bud 0 1 0 0 3 7 5 4 4 9 8 2 0 0 0 1 0 1 3 5 4 0 0 4
Bud Neck 0 0 0 0 0 0 0 3 2 1 1 2 0 0 0 0 0 0 0 0 1 1 2 1
Bud Site 1 0 1 2 5 13 18 10 40 33 21 11 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 18 18 19 36 27 26 28 21 22 45 9 13 22 13 20 14 26 0 2 3 1 2 1
Endoplasmic Reticulum 0 1 0 0 2 0 0 1 0 1 0 1 0 0 0 5 1 1 0 0 1 2 1 1
Endosome 0 6 4 1 2 2 1 0 0 3 0 0 0 0 0 7 6 11 0 9 10 2 4 10
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 3 1 1 0
Mitochondria 36 47 35 11 50 286 195 212 226 227 366 137 0 0 0 8 3 6 1 11 5 4 6 10
Nucleus 288 807 560 362 484 500 522 560 630 663 568 558 131 111 60 79 55 81 269 763 465 154 141 158
Nuclear Periphery 1 1 11 3 6 9 16 8 14 23 20 18 2 2 0 1 0 0 0 2 4 0 1 1
Nucleolus 29 38 90 17 39 134 200 214 285 296 270 320 15 3 1 4 2 6 18 65 115 36 30 51
Peroxisomes 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0
SpindlePole 1 8 5 9 8 1 4 5 5 11 5 3 2 1 1 7 5 10 1 8 8 9 4 8
Vac/Vac Membrane 3 13 1 3 2 3 6 2 6 5 2 1 0 0 1 12 17 19 15 40 21 16 16 22
Unique Cell Count 309 873 631 392 560 664 706 767 866 913 899 817 147 136 74 121 89 137 314 918 658 235 215 280
Labelled Cell Count 364 941 727 427 637 983 994 1047 1233 1295 1307 1062 163 139 76 144 104 161 314 918 658 235 215 280


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 6.1 5.7 5.4 5.3 4.3 4.9 4.9 4.7 5.0 4.2 5.2 7.0 6.9 6.9 6.1 6.5 6.4 6.0 6.2 6.4
Std Deviation (1e-4) 0.9 1.1 1.2 1.6 1.8 1.5 4.4 1.5 1.3 1.4 1.3 1.4 1.2 1.6 1.3 1.3 1.5 1.4 1.2 1.2 1.0
Intensity Change (Log2) -0.08 -0.12 -0.41 -0.23 -0.23 -0.27 -0.19 -0.45 -0.14 0.29 0.27 0.27 0.08 0.18 0.17 0.06 0.12 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 4.5 0 2.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.7 3.0 1.2 0.8 0.8 -0.5 -0.5 2.1 -2.4 3.3 6.3 0 6.3 0 7.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.1 2.3 0 0 0 0 9.9 0 6.5 -2.9 -2.8 0 0.5 0 -0.6
Nucleus 1.9 -1.2 -6.3 -6.9 -7.3 -7.6 -7.7 -11.2 -9.2 0.1 -2.3 -1.9 -6.6 -6.7 -8.5
Nuclear Periphery -1.3 -1.0 0 0 0 0 1.0 0 0.6 0 0 0 0 0 0
Nucleolus -5.0 -4.0 0 0 0 0 8.1 0 10.4 -1.3 -3.9 -3.1 -3.3 -3.2 -3.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2.0 1.1 0 0 0 0 0.8 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0