Standard name
Human Ortholog
Description Component of the Rpd3L histone deacetylase complex; involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus shares significant sequence identity with the human candidate tumor suppressor p33-ING1 and its isoform ING3

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.06 0 0 0 0 0 0.05 0 0.09 0.16 0.18 0.17 0.16 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.08 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.05 0.06 0 0.09 0.43 0.28 0.28 0.26 0.25 0.41 0.17 0 0 0 0.07 0 0 0 0 0 0 0 0
Nucleus 0.93 0.92 0.89 0.92 0.86 0.75 0.74 0.73 0.73 0.73 0.63 0.68 0.89 0.82 0.81 0.65 0.62 0.59 0.86 0.83 0.71 0.66 0.66 0.57
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 0.14 0 0.07 0.2 0.28 0.28 0.33 0.32 0.3 0.39 0.1 0 0 0 0 0 0.06 0.07 0.18 0.16 0.14 0.18
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.19 0.14 0.05 0 0 0.07 0.08 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 1
Bud 0 1 0 0 3 7 5 4 4 9 8 2 0 0 0 1 0 1 3 5 4 0 0 4
Bud Neck 0 0 0 0 0 0 0 3 2 1 1 2 0 0 0 0 0 0 0 0 1 1 2 1
Bud Site 1 0 1 2 5 13 18 10 40 33 21 11 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 18 18 19 36 27 26 28 21 22 45 9 13 22 13 20 14 26 0 2 3 1 2 1
Endoplasmic Reticulum 0 1 0 0 2 0 0 1 0 1 0 1 0 0 0 5 1 1 0 0 1 2 1 1
Endosome 0 6 4 1 2 2 1 0 0 3 0 0 0 0 0 7 6 11 0 9 10 2 4 10
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 3 1 1 0
Mitochondria 36 47 35 11 50 286 195 212 226 227 366 137 0 0 0 8 3 6 1 11 5 4 6 10
Nucleus 288 807 560 362 484 500 522 560 630 663 568 558 131 111 60 79 55 81 269 763 465 154 141 158
Nuclear Periphery 1 1 11 3 6 9 16 8 14 23 20 18 2 2 0 1 0 0 0 2 4 0 1 1
Nucleolus 29 38 90 17 39 134 200 214 285 296 270 320 15 3 1 4 2 6 18 65 115 36 30 51
Peroxisomes 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0
SpindlePole 1 8 5 9 8 1 4 5 5 11 5 3 2 1 1 7 5 10 1 8 8 9 4 8
Vac/Vac Membrane 3 13 1 3 2 3 6 2 6 5 2 1 0 0 1 12 17 19 15 40 21 16 16 22
Unique Cell Count 309 873 631 392 560 664 706 767 866 913 899 817 147 136 74 121 89 137 314 918 658 235 215 280
Labelled Cell Count 364 941 727 427 637 983 994 1047 1233 1295 1307 1062 163 139 76 144 104 161 314 918 658 235 215 280


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 6.1 5.7 5.4 5.3 4.3 4.9 4.9 4.7 5.0 4.2 5.2 7.0 6.9 6.9 6.1 6.5 6.4 6.0 6.2 6.4
Std Deviation (1e-4) 0.9 1.1 1.2 1.6 1.8 1.5 4.4 1.5 1.3 1.4 1.3 1.4 1.2 1.6 1.3 1.3 1.5 1.4 1.2 1.2 1.0
Intensity Change (Log2) -0.08 -0.12 -0.41 -0.23 -0.23 -0.27 -0.19 -0.45 -0.14 0.29 0.27 0.27 0.08 0.18 0.17 0.06 0.12 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 4.5 0 2.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.7 3.0 1.2 0.8 0.8 -0.5 -0.5 2.1 -2.4 3.3 6.3 0 6.3 0 7.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.1 2.3 0 0 0 0 9.9 0 6.5 -2.9 -2.8 0 0.5 0 -0.6
Nucleus 1.9 -1.2 -6.3 -6.9 -7.3 -7.6 -7.7 -11.2 -9.2 0.1 -2.3 -1.9 -6.6 -6.7 -8.5
Nuclear Periphery -1.3 -1.0 0 0 0 0 1.0 0 0.6 0 0 0 0 0 0
Nucleolus -5.0 -4.0 0 0 0 0 8.1 0 10.4 -1.3 -3.9 -3.1 -3.3 -3.2 -3.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2.0 1.1 0 0 0 0 0.8 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6837 1.9074 2.2918 1.8708 2.4917 2.1871 4.8981 4.1967 4.3839 4.4299 3.923 4.3154 3.5381 2.9834 3.0221 3.2585 2.3705 2.6952
Actin 0 0 0.0001 0 0.0002 0 0.0131 0 0.0111 0.008 0.0214 0.003 0.0401 0 0.0158 0.0009 0.061 0.0018
Bud 0.0001 0 0 0 0.0001 0 0.0002 0 0.0003 0.0001 0.0003 0 0.0007 0 0.0004 0.0001 0.0001 0.0001
Bud Neck 0.0002 0.0001 0.0002 0 0.001 0.0005 0.0009 0 0.0006 0.0001 0.0006 0.001 0.0009 0.0001 0.0014 0.0002 0.0005 0.0008
Bud Periphery 0.0007 0 0 0 0.0004 0 0.0007 0 0.001 0.0001 0.0005 0.0001 0.0013 0 0.0009 0.0001 0.0001 0.0001
Bud Site 0.0001 0 0.0002 0 0.0005 0 0.0012 0 0.001 0.0003 0.0008 0.0001 0.0024 0 0.0077 0.0001 0.0004 0.0001
Cell Periphery 0.0002 0 0 0 0.0002 0 0.0003 0 0.0003 0 0.0001 0 0.0002 0 0.0008 0 0 0
Cytoplasm 0.0001 0.0001 0.0002 0 0.0004 0 0.0021 0 0.0015 0.0002 0.0009 0.0002 0.0015 0 0.0006 0 0.0003 0.0001
Cytoplasmic Foci 0 0 0.0001 0 0.001 0 0.0061 0 0.0042 0.0304 0.0035 0.0002 0.0102 0 0.0005 0.0014 0.0053 0.0019
Eisosomes 0 0 0 0 0.0001 0 0.0001 0 0.0002 0.0001 0.0003 0.0001 0.0003 0 0.0005 0 0.0001 0
Endoplasmic Reticulum 0.0001 0 0 0 0.0002 0 0.0026 0 0.0022 0.0004 0.0016 0.0007 0.0026 0 0.0006 0.0001 0.0004 0
Endosome 0.0001 0 0.0002 0 0.0099 0 0.0089 0 0.0038 0.0102 0.0098 0.002 0.0124 0 0.0018 0.0084 0.0105 0.0013
Golgi 0 0 0.0001 0 0.0016 0 0.0039 0 0.0015 0.0119 0.0098 0.0012 0.0055 0 0.0011 0.0072 0.0029 0.0006
Lipid Particles 0 0 0.0007 0 0.0144 0 0.0125 0 0.0039 0.0371 0.0088 0.0019 0.0115 0 0.0022 0.0054 0.0339 0.0008
Mitochondria 0.0005 0 0.0009 0 0.0072 0 0.0048 0 0.0045 0.0036 0.0225 0.0004 0.0045 0.0001 0.0038 0.0258 0.0008 0.0002
None 0 0 0.0004 0 0.0008 0 0.0042 0 0.0009 0.0002 0.0009 0.0008 0.0031 0 0.0019 0.0001 0.0006 0.0003
Nuclear Periphery 0.0025 0.0005 0.0009 0.0001 0.0093 0.0001 0.0123 0.0002 0.0093 0.0005 0.0166 0.0045 0.0147 0.0002 0.0038 0.0002 0.0069 0.0007
Nucleolus 0.0287 0.0266 0.0272 0.0164 0.15 0.077 0.0408 0.0315 0.0275 0.0158 0.175 0.116 0.0361 0.0362 0.0299 0.035 0.1277 0.0923
Nucleus 0.9648 0.9663 0.9634 0.9831 0.7559 0.9214 0.8663 0.9662 0.9159 0.859 0.7017 0.862 0.8306 0.9622 0.92 0.9118 0.7336 0.8952
Peroxisomes 0 0 0.0003 0 0.0016 0 0.0072 0 0.0023 0.0205 0.0051 0.0001 0.0028 0 0.0004 0.0016 0.0028 0.002
Punctate Nuclear 0.0006 0.0063 0.0049 0.0004 0.0394 0.0007 0.0077 0.0019 0.0032 0.0014 0.0173 0.0052 0.0154 0.001 0.001 0.0002 0.0104 0.0015
Vacuole 0.0008 0 0.0002 0 0.0033 0 0.0024 0 0.0034 0.0002 0.0014 0.0002 0.0017 0 0.0037 0.0005 0.0009 0.0001
Vacuole Periphery 0.0003 0 0.0001 0 0.0025 0 0.0016 0 0.0015 0.0002 0.0011 0.0003 0.0016 0 0.0009 0.0007 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2227 18.8733 8.8451 12.928 15.1273 14.4374 11.299 11.8628 17.6922 14.5918
Translational Efficiency 1.9059 0.6967 1.2466 1.0787 1.013 1.0215 1.243 1.4427 0.8226 1.089

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Component of the Rpd3L histone deacetylase complex; involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus shares significant sequence identity with the human candidate tumor suppressor p33-ING1 and its isoform ING3
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Pho23

Pho23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pho23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available