Standard name | |
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Description | Topoisomerase II; relieves torsional strain in DNA by cleaving and re-sealing phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation; human homolog TOP2A implicated in cancers, and can complement yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 2 | 7 | 14 | 9 | 3 | 9 | 11 | 2 | 0 | 5 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 0 | 0 | 5 | 6 | 10 | 5 | 6 | 10 | 3 | 11 | 5 | 16 | 37 | 54 | 12 | 16 | 12 | 0 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 3 | 2 | 0 | 0 | 2 | 12 | 16 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 3 | 3 | 15 | 28 | 22 | 46 | 74 | 84 | 87 | 86 | 64 | 134 | 1 | 3 | 20 | 4 | 4 | 3 | 2 | 0 | 0 | 0 | 3 | 9 |
Nucleus | 332 | 65 | 63 | 107 | 169 | 186 | 274 | 266 | 203 | 172 | 164 | 241 | 346 | 326 | 329 | 230 | 218 | 164 | 324 | 68 | 76 | 142 | 303 | 253 |
Nuclear Periphery | 3 | 0 | 1 | 1 | 6 | 6 | 9 | 7 | 7 | 12 | 8 | 7 | 0 | 3 | 8 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 2 | 9 |
Nucleolus | 9 | 6 | 2 | 14 | 16 | 31 | 46 | 46 | 53 | 43 | 52 | 69 | 23 | 43 | 85 | 17 | 20 | 34 | 13 | 0 | 0 | 11 | 56 | 81 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 1 | 5 | 7 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 5 | 5 |
Vac/Vac Membrane | 2 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 1 | 1 | 3 | 2 | 3 | 1 | 8 | 4 | 8 | 3 | 1 | 2 | 7 | 84 | 151 |
Unique Cell Count | 347 | 70 | 66 | 121 | 193 | 226 | 316 | 327 | 261 | 236 | 211 | 325 | 374 | 394 | 453 | 263 | 250 | 210 | 350 | 72 | 82 | 166 | 478 | 544 |
Labelled Cell Count | 353 | 74 | 81 | 156 | 222 | 283 | 420 | 424 | 370 | 321 | 311 | 472 | 392 | 426 | 520 | 276 | 264 | 230 | 350 | 72 | 82 | 166 | 478 | 544 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 9.3 | 6.8 | 5.5 | 5.9 | 6.0 | 5.9 | 5.5 | 5.2 | 4.9 | 5.0 | 4.7 | 4.4 | 9.9 | 10.3 | 8.9 | 16.7 | 17.5 | 18.7 | 8.6 | 8.4 | 8.6 |
Std Deviation (1e-4) | 2.0 | 1.6 | 1.0 | 1.5 | 1.5 | 1.4 | 1.7 | 1.2 | 1.6 | 1.5 | 1.1 | 1.3 | 3.2 | 4.3 | 4.0 | 5.3 | 6.9 | 7.0 | 1.9 | 2.0 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.14 | 0.09 | 0.0 | -0.06 | -0.18 | -0.14 | -0.23 | -0.33 | 0.84 | 0.91 | 0.7 | 1.6 | 1.67 | 1.77 | 0.64 | 0.61 | 0.64 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 3.0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.1 | -2.3 | -0.4 | 0.1 | 0.5 | 0 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.6 | -1.8 | -2.7 | -2.0 | -2.8 | -3.3 | -3.9 | -3.3 | -3.8 | -0.9 | -2.6 | -4.0 | -1.9 | -1.9 | -3.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.0 | 0 | 2.4 | 2.6 | 2.5 | 0 | 3.1 | 0 | 0 | 0 | 2.0 | 3.2 | 0 | 0 | 2.8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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