Standard name
Description Topoisomerase II; relieves torsional strain in DNA by cleaving and re-sealing phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation; human homolog TOP2A implicated in cancers, and can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.09 0.12 0.05 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.23 0.23 0.11 0.2 0.23 0.26 0.33 0.36 0.3 0.41 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.96 0.93 0.95 0.88 0.88 0.82 0.87 0.81 0.78 0.73 0.78 0.74 0.93 0.83 0.73 0.87 0.87 0.78 0.93 0.95 0.93 0.86 0.63 0.47
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.09 0 0.12 0.08 0.14 0.15 0.14 0.2 0.18 0.25 0.21 0.06 0.11 0.19 0.06 0.08 0.16 0 0 0 0.07 0.12 0.15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.18 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 1 1 0 0 0 1 1 1 0 1 0 0 0 0 1 0 0 0 3 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 1 2 7 14 9 3 9 11 2 0 5 1 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Cytoplasm 3 0 0 5 6 10 5 6 10 3 11 5 16 37 54 12 16 12 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 2 0 0 0 0 1 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 2 3 0 0 3 2 0 0 2 12 16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 2
Mitochondria 3 3 15 28 22 46 74 84 87 86 64 134 1 3 20 4 4 3 2 0 0 0 3 9
Nucleus 332 65 63 107 169 186 274 266 203 172 164 241 346 326 329 230 218 164 324 68 76 142 303 253
Nuclear Periphery 3 0 1 1 6 6 9 7 7 12 8 7 0 3 8 2 1 2 0 0 0 0 2 9
Nucleolus 9 6 2 14 16 31 46 46 53 43 52 69 23 43 85 17 20 34 13 0 0 11 56 81
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 6 1 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 1 0 2 0 1 0 1 1 1 5 7 0 0 1 0 0 0 1 5 5
Vac/Vac Membrane 2 0 0 0 0 1 3 1 0 1 1 3 2 3 1 8 4 8 3 1 2 7 84 151
Unique Cell Count 347 70 66 121 193 226 316 327 261 236 211 325 374 394 453 263 250 210 350 72 82 166 478 544
Labelled Cell Count 353 74 81 156 222 283 420 424 370 321 311 472 392 426 520 276 264 230 350 72 82 166 478 544


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.3 6.8 5.5 5.9 6.0 5.9 5.5 5.2 4.9 5.0 4.7 4.4 9.9 10.3 8.9 16.7 17.5 18.7 8.6 8.4 8.6
Std Deviation (1e-4) 2.0 1.6 1.0 1.5 1.5 1.4 1.7 1.2 1.6 1.5 1.1 1.3 3.2 4.3 4.0 5.3 6.9 7.0 1.9 2.0 1.8
Intensity Change (Log2) – – – 0.09 0.14 0.09 0.0 -0.06 -0.18 -0.14 -0.23 -0.33 0.84 0.91 0.7 1.6 1.67 1.77 0.64 0.61 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 2.6 3.0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 -2.3 -0.4 0.1 0.5 0 2.1 0 0 0 0 0 0 0 0
Nucleus -1.6 -1.8 -2.7 -2.0 -2.8 -3.3 -3.9 -3.3 -3.8 -0.9 -2.6 -4.0 -1.9 -1.9 -3.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.0 0 2.4 2.6 2.5 0 3.1 0 0 0 2.0 3.2 0 0 2.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Top2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available