Standard name
Description Putative S-adenosylmethionine-dependent lysine methyltransferase; responsible for modifying Lys-390 in translational elongation factor EF-1 alpha (eEF1A); has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.96 0.98 0.97 0.95 0.95 0.93 0.92 0.81 0.86 0.63 0.74 0.99 1.0 1.0 0.97 0.82 0.9 0.77 0.9 0.9 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.13 0 0 0 0.05 0.07 0.06 0.32 0.19 0.51 0.36 0 0 0 0 0.11 0 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 1 0 1 0 0 1
Bud 0 1 0 0 2 1 0 0 4 5 4 5 1 1 0 0 0 0 3 3 3 2
Bud Neck 0 0 3 3 9 6 7 11 5 9 1 3 1 1 1 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0
Cell Periphery 1 0 1 2 3 3 1 2 0 2 1 0 1 2 0 2 0 0 0 1 0 1
Cytoplasm 134 128 151 160 182 222 265 236 210 236 84 172 170 282 281 178 124 121 138 192 244 237
Endoplasmic Reticulum 2 0 0 0 1 0 0 0 0 0 0 1 0 0 0 14 0 0 1 0 1 0
Endosome 0 0 0 0 0 3 1 0 0 0 0 0 0 0 0 0 2 0 1 1 1 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0
Mitochondria 1 18 0 5 1 11 20 16 83 53 68 83 0 0 0 7 16 5 17 2 5 11
Nucleus 0 2 0 1 1 1 5 3 1 4 5 4 1 1 1 2 0 0 1 0 1 1
Nuclear Periphery 0 0 1 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 3 2 7 10 2 4 3 12 0 0 0 2 1 0 4 1 1 5
Vac/Vac Membrane 0 0 1 1 0 2 0 0 1 1 0 1 0 0 0 3 2 1 1 1 3 0
Unique Cell Count 138 134 154 165 192 234 284 256 259 275 134 233 171 283 281 183 153 136 179 214 271 275
Labelled Cell Count 139 149 157 174 203 251 307 279 306 316 166 286 174 287 283 208 153 136 179 214 271 275


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 2.7 4.9 4.6 5.0 4.0 4.3 4.5 3.5 3.9 3.4 3.7 4.4 4.8 4.5 3.9 4.5 5.0 5.2 5.3 5.0
Std Deviation (1e-4) 0.3 0.3 1.4 1.0 1.5 0.9 1.9 1.7 1.5 1.8 1.2 1.6 0.8 1.4 1.2 1.6 1.1 1.6 1.8 1.7 1.6
Intensity Change (Log2) -0.09 0.02 -0.29 -0.18 -0.13 -0.49 -0.34 -0.52 -0.42 -0.17 -0.02 -0.13 -0.31 -0.13 0.04 0.1 0.11 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Efm6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available