Standard name | |
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Description | Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; shuttles between cytosol and nucleus; mediates delivery of misfolded proteins into the nucleus for degradation; involved in proteasomal degradation of misfolded cytosolic proteins; protein abundance increases in response to DNA replication stress; polyQ aggregates sequester Sis1p and interfere with clearance of misfolded proteins; similar to bacterial DnaJ proteins and mammalian DnaJB1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 5 | 4 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 2 | 2 | 4 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 1 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 11 | 5 | 5 | 10 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 4 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 46 | 93 | 43 | 11 | 33 | 49 | 63 | 43 | 42 | 56 | 46 | 72 | 37 | 83 | 45 | 14 | 4 | 1 | 37 | 83 | 53 | 29 | 18 | 19 |
Endoplasmic Reticulum | 2 | 9 | 2 | 3 | 3 | 5 | 14 | 15 | 7 | 9 | 4 | 11 | 0 | 2 | 1 | 4 | 1 | 1 | 2 | 6 | 5 | 1 | 0 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 8 | 11 | 5 | 9 | 11 |
Golgi | 4 | 3 | 25 | 9 | 21 | 10 | 13 | 8 | 8 | 18 | 1 | 14 | 11 | 16 | 13 | 13 | 0 | 2 | 0 | 3 | 4 | 2 | 17 | 28 |
Mitochondria | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 1 | 3 | 1 | 7 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleus | 0 | 2 | 2 | 5 | 5 | 12 | 5 | 10 | 10 | 9 | 16 | 10 | 1 | 2 | 0 | 2 | 0 | 2 | 37 | 79 | 51 | 48 | 56 | 65 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 2 | 0 | 1 | 2 |
Nucleolus | 4 | 3 | 8 | 6 | 8 | 8 | 7 | 8 | 11 | 6 | 11 | 1 | 5 | 7 | 4 | 8 | 3 | 8 | 0 | 1 | 3 | 5 | 5 | 5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 2 | 2 | 2 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 8 | 4 | 11 | 5 |
Vac/Vac Membrane | 12 | 26 | 67 | 20 | 77 | 52 | 41 | 65 | 42 | 34 | 30 | 46 | 35 | 34 | 53 | 24 | 12 | 6 | 13 | 54 | 43 | 35 | 55 | 57 |
Unique Cell Count | 64 | 131 | 135 | 51 | 141 | 131 | 139 | 144 | 125 | 133 | 114 | 149 | 81 | 135 | 107 | 58 | 19 | 17 | 109 | 277 | 204 | 152 | 202 | 223 |
Labelled Cell Count | 68 | 136 | 147 | 54 | 150 | 139 | 148 | 155 | 133 | 142 | 124 | 160 | 89 | 145 | 117 | 67 | 21 | 21 | 109 | 277 | 204 | 152 | 202 | 223 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 57.1 | 74.7 | 56.3 | 51.9 | 51.0 | 47.6 | 44.3 | 46.1 | 44.6 | 45.4 | 43.6 | 48.5 | 59.0 | 69.9 | 73.0 | 106.9 | 107.3 | 111.6 | 55.8 | 66.1 | 77.8 |
Std Deviation (1e-4) | 13.7 | 18.8 | 14.2 | 23.2 | 14.9 | 16.9 | 13.6 | 16.7 | 17.2 | 13.0 | 15.5 | 15.9 | 14.6 | 15.8 | 15.4 | 39.6 | 39.9 | 35.4 | 11.6 | 20.8 | 17.7 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.14 | -0.24 | -0.35 | -0.29 | -0.33 | -0.31 | -0.37 | -0.21 | 0.07 | 0.31 | 0.38 | 0.93 | 0.93 | 0.99 | -0.01 | 0.23 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.4 | -1.6 | 1.0 | 2.3 | -0.4 | 0.3 | 1.7 | 1.4 | 2.8 | 2.0 | 4.9 | 1.6 | -1.1 | -1.0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 3.0 | 3.1 | 0 | 2.2 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -0.1 | -0.8 | -2.6 | -2.2 | -3.4 | -2.9 | -1.1 | -4.5 | -2.2 | -0.9 | -1.5 | -1.4 | 0.6 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 2.8 | 0 | 2.2 | 2.5 | 2.2 | 3.8 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | -0.1 | 0.1 | -0.3 | -0.1 | 0.9 | -0.5 | 1.1 | 0 | 0 | -0.3 | -0.8 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.3 | 0.8 | -1.6 | -3.4 | -0.8 | -2.6 | -4.1 | -3.8 | -3.2 | -0.9 | -4.2 | 0 | -1.1 | 1.1 | -1.1 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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