Standard name
Description 73 kDa subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive; SNF12 has a paralog, RSC6, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 0.06 0.05 0 0 0 0.07 0.14 0.21 0.06 0.06 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.08 0 0 0 0.06 0.07 0 0.09 0.06 0.27 0.12 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.9 0.9 0.95 0.9 0.91 0.9 0.87 0.86 0.83 0.86 0.76 0.79 0.9 0.85 0.77 0.82 0.8 0.65 0.78 0.82 0.77 0.65 0.75 0.71
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0 0.08 0.08 0.13 0.18 0.16 0.26 0.22 0 0 0 0.08 0.09 0.09 0.11 0.06 0.11 0.13 0.07 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.08 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.09 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 0 0 0 2 3 1 0 0 5
Bud Neck 0 0 0 1 0 0 0 1 1 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 1 3 4 5 5 10 12 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 7 4 9 10 13 14 28 16 9 9 6 13 31 58 11 9 23 0 0 0 0 1 4
Endoplasmic Reticulum 0 2 1 0 1 0 1 0 0 0 0 0 0 0 0 2 0 3 0 0 0 0 0 1
Endosome 1 2 1 4 2 0 5 4 0 1 0 1 0 1 0 5 4 5 3 6 2 0 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 4 0 1 1 0 1
Mitochondria 27 22 0 6 2 23 25 9 29 20 110 50 1 1 1 8 2 3 1 3 0 1 1 2
Nucleus 218 251 147 161 261 319 331 415 265 281 314 324 167 192 214 142 116 80 201 238 124 40 124 146
Nuclear Periphery 1 1 0 0 2 2 3 3 0 0 0 2 0 0 2 0 0 0 0 0 0 0 1 0
Nucleolus 20 5 0 5 12 29 32 63 57 51 107 91 3 2 12 14 13 11 27 16 18 7 12 20
Peroxisomes 1 1 0 0 0 0 1 0 0 0 0 2 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 3 3 12 5 8 6 9 8 3 5 3 9 11 3 9 4 4 9 3 3 3 2 2 2
Vac/Vac Membrane 1 7 1 1 1 3 0 5 2 4 0 7 1 2 4 8 8 11 10 13 5 2 7 12
Unique Cell Count 242 280 154 179 286 354 379 483 319 327 413 408 185 227 278 174 145 123 258 292 162 62 166 206
Labelled Cell Count 274 301 166 193 299 396 424 541 378 377 554 506 196 232 301 196 156 145 258 292 162 62 166 206


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 5.7 6.7 6.6 7.0 5.9 6.4 6.7 5.6 5.9 5.1 5.5 6.9 7.3 6.7 8.5 9.4 8.8 7.0 6.8 7.4
Std Deviation (1e-4) 0.7 1.1 1.4 2.9 1.6 1.3 4.7 1.8 1.6 1.2 1.3 1.3 1.3 2.0 1.7 2.0 2.5 4.0 2.6 1.9 2.2
Intensity Change (Log2) -0.04 0.06 -0.19 -0.07 -0.0 -0.25 -0.2 -0.39 -0.28 0.03 0.11 0.0 0.33 0.48 0.39 0.05 0.01 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 0 0.6 0.6 1.6 1.2 0 0 0 1.9 3.7 5.2 1.6 1.5 4.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.2 3.3 0 3.9 3.1 7.1 4.6 0 0 0 0 0 0
Nucleus -1.9 -1.6 -2.0 -2.8 -3.2 -3.8 -3.1 -5.3 -4.6 -1.8 -3.3 -5.0 -3.9 -4.1 -6.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.7 3.7 4.7 5.6 5.2 7.0 6.4 0 0 0 3.6 3.8 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -2.1 -2.4 -3.4 -2.9 0 0 -3.5 0 -3.1 -0.7 -3.2 -2.1 -2.3 -1.9 -0.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snf12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available