Standard name
Description Protein involved in methylation of tRNA, rRNA, and translation factors; also involved in ribosome biogenesis; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; N7-methylates G1575 of 18S rRNA as complex with Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; relative distribution to the nucleus increases upon DNA replication stress; functional homolog of human TRMT112

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 0.39 0.58 0.68 0.69 0.56 0.64 0.69 0.5 0.55 0.64 0.64 0.59 0.95 0.93 0.91 0.91 0.91 0.88 0.86 0.8 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.11 0 0 0 0 0 0 0 0 0.12 0.15 0.17 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.05 0.07 0.12 0.14 0.17 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.05 0 0.06 0.05 0 0.12 0.1 0 0 0 0 0 0 0 0 0 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.22 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.31 0.44 0.3 0.24 0.34 0.24 0.17 0.26 0.21 0.41 0.41 0.44 0.07 0.08 0.08 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 2 1 0 2 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 1 0 0 5 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 1 2 0 1 3 0 1 2 2 0 1 3 0 1 0 0 3 1 0 0 0 0 0 0
Cytoplasm 382 400 101 48 114 134 218 200 136 159 111 125 66 105 100 144 110 108 358 357 94 81 161 154
Endoplasmic Reticulum 1 0 0 4 0 0 3 4 1 3 4 3 0 1 0 2 5 10 2 0 1 0 2 3
Endosome 0 0 0 10 1 1 3 1 0 2 1 1 0 0 1 0 0 0 1 1 0 0 1 3
Golgi 0 0 0 14 5 1 4 4 0 7 4 5 12 24 29 2 1 1 1 0 0 0 0 0
Mitochondria 0 0 0 4 1 15 17 25 26 31 38 37 0 2 0 1 2 0 0 1 0 0 0 0
Nucleus 5 0 1 1 6 9 13 23 10 8 26 23 0 0 2 0 0 0 15 16 3 5 5 5
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 2
Nucleolus 0 0 0 27 2 1 2 1 1 2 1 7 4 4 2 2 1 0 0 0 0 0 1 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 3
Vac/Vac Membrane 5 4 2 38 87 60 74 123 52 38 58 48 42 68 75 10 9 10 9 8 3 4 7 8
Unique Cell Count 390 404 103 122 196 198 314 359 214 229 220 227 103 165 169 152 118 119 393 393 108 95 201 192
Labelled Cell Count 394 406 105 147 219 223 338 384 229 253 246 254 124 205 209 161 131 130 393 393 108 95 201 192


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.6 27.3 13.0 17.0 15.5 13.4 12.5 13.4 12.6 13.2 12.8 13.4 25.4 24.8 22.6 41.9 41.8 37.4 22.3 28.2 26.7
Std Deviation (1e-4) 4.4 4.8 2.6 3.8 3.1 2.9 3.0 3.2 3.0 3.5 3.1 3.4 5.6 4.9 4.0 14.3 13.9 11.9 4.7 6.0 6.4
Intensity Change (Log2) 0.39 0.26 0.05 -0.06 0.05 -0.04 0.02 -0.02 0.05 0.97 0.93 0.8 1.69 1.69 1.53 0.78 1.12 1.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -9.3 -7.3 -6.0 -5.9 -8.0 -6.6 -5.9 -8.4 -7.8 -6.2 -6.5 -7.1 -1.3 -1.7 -2.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 3.6 0 0 0 0 0 0 0 0 3.6 4.1 4.4 0 0 0
Mitochondria 0 0 2.9 0 2.8 3.7 3.9 4.5 4.3 0 0 0 0 0 0
Nucleus 0 0 0 0 2.2 0 0 3.3 3.0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 5.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 5.7 7.6 5.8 4.9 6.5 5.0 3.8 5.3 4.5 6.8 7.1 7.5 0 1.9 2.1


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trm112-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available