Standard name
Description RNase of the DEDD superfamily; subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0.05 0 0 0.05 0.08 0.09 0.08 0.07 0 0 0 0 0.08 0.07 0.09 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.91 0.92 0.87 0.74 0.67 0.62 0.63 0.54 0.59 0.53 0.98 0.97 0.98 0.92 0.88 0.91 0.93 0.95 0.88 0.95 0.93 0.91
Endoplasmic Reticulum 0 0 0.1 0.07 0.06 0 0.08 0.11 0.13 0.1 0.12 0.11 0 0 0 0 0 0 0 0 0.09 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0 0.07 0.29 0.34 0.26 0.29 0.43 0.36 0.4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 2 2 3 8 6 7 4 4 10 3 0 1 3 1 0 0 0 0 0 0 1 1
Bud 0 1 0 0 0 0 1 4 0 4 0 1 0 0 0 0 0 0 0 0 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 11 9 4 7 17 24 28 25 14 9 2 17 7 10 11 11 0 0 0 0 0 0
Cytoplasm 147 231 202 173 172 199 247 181 202 174 121 123 329 417 424 121 134 106 148 219 201 293 394 343
Endoplasmic Reticulum 0 1 23 13 12 12 28 33 41 31 25 25 8 12 2 5 4 1 2 4 19 5 7 16
Endosome 0 0 0 1 0 0 1 1 0 0 0 0 2 3 1 0 0 0 0 0 0 1 4 2
Golgi 0 1 0 1 2 4 4 5 4 0 2 1 0 0 1 1 1 2 1 1 0 0 0 1
Mitochondria 14 0 1 5 13 78 125 77 94 139 75 92 0 0 1 3 5 2 1 0 1 1 4 4
Nucleus 0 0 2 0 0 0 0 0 0 0 0 3 0 1 2 1 0 0 0 2 0 0 2 2
Nuclear Periphery 0 0 0 1 0 0 0 1 2 2 2 2 0 0 0 0 0 0 0 0 0 1 1 0
Nucleolus 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 1 4 5 5 8 21 9 10 8 4 0 3 5 0 11 6 2 1 1 2 2 2
Unique Cell Count 153 233 222 188 198 268 370 294 319 322 206 232 336 428 433 131 152 116 160 232 229 309 426 380
Labelled Cell Count 163 235 242 209 212 313 437 354 384 390 257 263 341 454 446 142 166 128 160 232 229 309 426 380


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.4 14.6 12.8 12.3 11.6 10.5 10.7 10.7 10.7 10.3 10.5 10.0 12.8 12.4 12.1 14.8 13.9 15.0 13.1 13.8 14.6
Std Deviation (1e-4) 1.5 1.8 1.6 1.6 2.0 1.9 2.1 2.2 2.4 2.4 2.5 2.6 1.7 1.6 1.3 3.5 2.6 3.4 1.9 2.1 2.3
Intensity Change (Log2) -0.06 -0.14 -0.28 -0.26 -0.26 -0.26 -0.31 -0.28 -0.35 0.0 -0.05 -0.08 0.21 0.13 0.23 0.04 0.1 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 2.5 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 -1.6 -1.4 -0.2 1.4 1.7 1.3 0.8 -0.6 -3.3 -0.6 -2.5 1.0 0.9 1.6
Cytoplasm 0.4 -1.4 -4.8 -6.7 -7.6 -7.3 -9.2 -7.7 -9.0 3.7 3.7 4.1 0.4 -0.9 0.1
Endoplasmic Reticulum -1.2 -1.6 -2.5 -1.2 0.3 0.9 -0.3 0.6 0.1 -4.0 -4.0 -6.3 -2.2 -2.8 -3.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.5 8.6 9.6 8.1 8.7 11.2 9.7 10.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.7 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pop2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available