Standard name
Human Ortholog
Description RNase of the DEDD superfamily; subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0.05 0 0 0.05 0.08 0.09 0.08 0.07 0 0 0 0 0.08 0.07 0.09 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.91 0.92 0.87 0.74 0.67 0.62 0.63 0.54 0.59 0.53 0.98 0.97 0.98 0.92 0.88 0.91 0.93 0.95 0.88 0.95 0.93 0.91
Endoplasmic Reticulum 0 0 0.1 0.07 0.06 0 0.08 0.11 0.13 0.1 0.12 0.11 0 0 0 0 0 0 0 0 0.09 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0 0.07 0.29 0.34 0.26 0.29 0.43 0.36 0.4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 2 2 3 8 6 7 4 4 10 3 0 1 3 1 0 0 0 0 0 0 1 1
Bud 0 1 0 0 0 0 1 4 0 4 0 1 0 0 0 0 0 0 0 0 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 11 9 4 7 17 24 28 25 14 9 2 17 7 10 11 11 0 0 0 0 0 0
Cytoplasm 147 231 202 173 172 199 247 181 202 174 121 123 329 417 424 121 134 106 148 219 201 293 394 343
Endoplasmic Reticulum 0 1 23 13 12 12 28 33 41 31 25 25 8 12 2 5 4 1 2 4 19 5 7 16
Endosome 0 0 0 1 0 0 1 1 0 0 0 0 2 3 1 0 0 0 0 0 0 1 4 2
Golgi 0 1 0 1 2 4 4 5 4 0 2 1 0 0 1 1 1 2 1 1 0 0 0 1
Mitochondria 14 0 1 5 13 78 125 77 94 139 75 92 0 0 1 3 5 2 1 0 1 1 4 4
Nucleus 0 0 2 0 0 0 0 0 0 0 0 3 0 1 2 1 0 0 0 2 0 0 2 2
Nuclear Periphery 0 0 0 1 0 0 0 1 2 2 2 2 0 0 0 0 0 0 0 0 0 1 1 0
Nucleolus 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 1 4 5 5 8 21 9 10 8 4 0 3 5 0 11 6 2 1 1 2 2 2
Unique Cell Count 153 233 222 188 198 268 370 294 319 322 206 232 336 428 433 131 152 116 160 232 229 309 426 380
Labelled Cell Count 163 235 242 209 212 313 437 354 384 390 257 263 341 454 446 142 166 128 160 232 229 309 426 380


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.4 14.6 12.8 12.3 11.6 10.5 10.7 10.7 10.7 10.3 10.5 10.0 12.8 12.4 12.1 14.8 13.9 15.0 13.1 13.8 14.6
Std Deviation (1e-4) 1.5 1.8 1.6 1.6 2.0 1.9 2.1 2.2 2.4 2.4 2.5 2.6 1.7 1.6 1.3 3.5 2.6 3.4 1.9 2.1 2.3
Intensity Change (Log2) – – – -0.06 -0.14 -0.28 -0.26 -0.26 -0.26 -0.31 -0.28 -0.35 0.0 -0.05 -0.08 0.21 0.13 0.23 0.04 0.1 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 2.5 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 -1.6 -1.4 -0.2 1.4 1.7 1.3 0.8 -0.6 -3.3 -0.6 -2.5 1.0 0.9 1.6
Cytoplasm 0.4 -1.4 -4.8 -6.7 -7.6 -7.3 -9.2 -7.7 -9.0 3.7 3.7 4.1 0.4 -0.9 0.1
Endoplasmic Reticulum -1.2 -1.6 -2.5 -1.2 0.3 0.9 -0.3 0.6 0.1 -4.0 -4.0 -6.3 -2.2 -2.8 -3.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.5 8.6 9.6 8.1 8.7 11.2 9.7 10.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.7 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.1583 10.4764 10.0815 9.2717 9.5248 9.9534 6.4531 9.784 8.8303 8.495 6.5995 8.9828 8.6954 10.8616 9.7049 9.3704 9.2326 9.6205
Actin 0.003 0.001 0.0049 0.0006 0.0011 0.0019 0.0452 0.0018 0.0114 0.0005 0.0193 0.001 0.0171 0.001 0.0106 0.0052 0.0028 0.0005
Bud 0.0005 0.0003 0.0006 0.0005 0.0003 0.0005 0.001 0.0027 0.0014 0.0004 0.0055 0.0004 0.0005 0.0004 0.0005 0.0045 0.0016 0.0003
Bud Neck 0.0004 0.0002 0.0004 0.0005 0.0005 0.0013 0.0022 0.0003 0.0005 0.0005 0.0018 0.0012 0.0008 0.0002 0.0009 0.0008 0.0007 0.001
Bud Periphery 0.0005 0.0001 0.0002 0.0002 0 0.0004 0.0011 0.0012 0.0009 0.0001 0.0058 0.0004 0.0004 0.0001 0.0003 0.0014 0.0014 0.0002
Bud Site 0.0016 0.0011 0.0011 0.0003 0.0002 0.0002 0.0071 0.0036 0.0015 0.0002 0.0246 0.0002 0.001 0.0005 0.0009 0.0017 0.0003 0.0001
Cell Periphery 0.0013 0.0005 0.0002 0.0001 0.0001 0.0002 0.0004 0.0003 0.0001 0.0001 0.0007 0.0001 0.0007 0.0001 0.0001 0.0002 0.0001 0.0001
Cytoplasm 0.6803 0.9266 0.8646 0.8229 0.8125 0.8044 0.634 0.9023 0.8314 0.8336 0.5193 0.8049 0.7682 0.935 0.8593 0.8039 0.7646 0.846
Cytoplasmic Foci 0.0127 0.009 0.012 0.0142 0.034 0.0197 0.0283 0.0181 0.0324 0.0177 0.1148 0.0235 0.017 0.0098 0.0158 0.0193 0.0283 0.017
Eisosomes 0 0 0 0 0 0 0.0005 0 0 0 0.0001 0 0.0004 0 0.0001 0 0 0
Endoplasmic Reticulum 0.1838 0.0238 0.0534 0.0124 0.0197 0.0678 0.0802 0.0187 0.0228 0.0115 0.0093 0.0516 0.0921 0.024 0.0325 0.0387 0.0281 0.0538
Endosome 0.0586 0.0239 0.0363 0.0941 0.1012 0.0692 0.0938 0.031 0.0553 0.1045 0.1258 0.0773 0.0626 0.0189 0.0565 0.0695 0.0844 0.0607
Golgi 0.0128 0.003 0.0065 0.0038 0.0098 0.0124 0.0194 0.0052 0.0196 0.0042 0.0699 0.0102 0.01 0.0035 0.0056 0.0044 0.0144 0.0055
Lipid Particles 0.0008 0.0001 0.0001 0.0001 0.0002 0.0001 0.0107 0.0003 0.0014 0.0001 0.0355 0.0002 0.0017 0.0001 0.0005 0.0003 0.0007 0.0001
Mitochondria 0.0037 0.0004 0.0045 0.0011 0.001 0.0122 0.024 0.0037 0.0039 0.0012 0.0305 0.017 0.0049 0.0015 0.0018 0.0025 0.0311 0.0052
None 0.0024 0.0014 0.001 0.0006 0.0008 0.0009 0.0042 0.0023 0.0009 0.0006 0.0018 0.0007 0.0026 0.0004 0.0007 0.0014 0.0009 0.0006
Nuclear Periphery 0.0088 0.001 0.0031 0.0051 0.0016 0.0019 0.0169 0.001 0.0014 0.002 0.0027 0.0023 0.0043 0.0009 0.0026 0.0042 0.0223 0.0015
Nucleolus 0 0 0 0 0 0 0.0004 0.0001 0 0 0.0003 0 0.0001 0 0 0 0.0004 0
Nucleus 0.002 0.0009 0.0012 0.0018 0.0009 0.001 0.0038 0.0009 0.0011 0.0012 0.0025 0.001 0.0018 0.0005 0.0012 0.0018 0.005 0.001
Peroxisomes 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0049 0.0004 0.0053 0.0001 0.0187 0.0004 0.0008 0.0001 0.0003 0.0002 0.0003 0.0001
Punctate Nuclear 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0053 0.0002 0.0003 0.0001 0.0031 0.0001 0.0012 0.0001 0.0003 0.0004 0.0004 0.0001
Vacuole 0.0219 0.0054 0.0072 0.0296 0.0128 0.0036 0.0121 0.0049 0.0058 0.0147 0.0056 0.005 0.0097 0.0023 0.0075 0.031 0.0056 0.0039
Vacuole Periphery 0.0045 0.0011 0.0021 0.0118 0.0029 0.002 0.0044 0.001 0.0023 0.0066 0.0024 0.0024 0.0022 0.0007 0.002 0.0085 0.0065 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.0513 34.8489 36.5405 36.3557 31.8646 35.574 38.6827 37.4821 36.1621 39.9193
Translational Efficiency 1.5712 1.6731 1.4484 1.2535 1.5183 1.5797 1.4691 1.3298 1.5554 1.2825

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description RNase of the DEDD superfamily; subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation
Localization
Cell Percentages cytoplasm (86%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Pop2

Pop2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pop2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available