Standard name
Description Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.05 0.13 0.16 0.15 0 0.1 0.05 0.08 0.07 0.08 0.33 0.16 0.09 0.05 0.12 0.05 0 0 0 0.05 0.07
Bud 0 0 0 0 0 0.06 0 0 0 0 0 0.09 0.1 0.11 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0.11 0.09 0 0 0 0 0 0
Cytoplasm 0.49 0.73 0.51 0.47 0.44 0.29 0.3 0.2 0.24 0.22 0.2 0.25 0.19 0.32 0.53 0.55 0.21 0.44 0.21 0.17 0.12 0.1
Endoplasmic Reticulum 0.22 0.21 0.37 0.23 0.27 0.07 0.23 0.13 0.12 0.1 0.16 0.21 0 0.09 0 0.06 0.28 0.23 0.19 0.29 0.27 0.3
Endosome 0 0 0 0 0.06 0 0 0 0 0 0 0.08 0.32 0.24 0 0.06 0 0.05 0.05 0 0.05 0.07
Golgi 0 0 0 0.06 0.09 0 0.1 0 0 0 0.05 0.28 0.1 0.05 0.05 0.12 0.23 0.08 0.16 0.25 0.34 0.31
Mitochondria 0.19 0.06 0 0.28 0.14 0.61 0.22 0.63 0.58 0.65 0.64 0.14 0.13 0.14 0.05 0.09 0.11 0.08 0.27 0.18 0.11 0.12
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0.05 0.31 0.18 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.08 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0 0.11 0.22 0.16 0.11 0.12 0.09 0 0 0.05 0.37 0.12 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 7 19 16 26 12 29 17 37 29 23 55 30 20 1 4 7 3 8 3 12 14
Bud 2 2 2 2 2 16 3 13 15 14 6 15 20 23 0 0 1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 5 6 5 4 7 6 12 12 4 0 0 5 2 3 0 0 1 0 0 1
Cytoplasm 42 107 75 46 78 77 90 69 105 89 60 43 36 70 10 18 29 80 41 23 27 21
Endoplasmic Reticulum 19 31 54 22 48 20 68 45 52 41 47 35 4 19 0 2 40 41 38 40 63 64
Endosome 2 0 2 1 10 4 13 15 13 14 5 13 61 51 0 2 5 8 9 5 12 14
Golgi 2 0 4 6 16 3 29 5 17 13 16 47 20 11 1 4 33 13 31 34 79 66
Mitochondria 16 9 5 27 24 163 66 222 258 266 194 24 25 30 1 3 16 15 53 24 25 26
Nucleus 1 1 0 2 3 6 2 1 3 0 1 0 0 0 0 1 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 1 0 4 0 1 3 2 0 0 0 0 1 0 0 0 1 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 1 1 2 2 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 8 60 40 0 0 1 1 0 0 0 1
SpindlePole 0 0 0 0 0 0 1 0 1 0 0 1 25 17 0 0 0 0 0 0 0 1
Vac/Vac Membrane 5 0 5 4 7 30 66 57 50 48 26 1 3 10 7 4 5 12 6 2 8 2
Unique Cell Count 86 146 146 97 176 269 302 352 444 410 302 169 193 217 19 33 144 183 197 140 233 218
Labelled Cell Count 95 159 171 132 219 336 374 454 563 527 386 245 286 299 22 41 144 183 197 140 233 218


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 8.3 8.4 7.8 9.3 6.2 8.5 7.6 8.0 7.6 7.6 9.6 9.7 9.5 10.6 10.7 8.2 8.6 8.9
Std Deviation (1e-4) 1.5 1.2 1.0 1.0 1.5 1.3 1.4 1.3 1.4 1.4 1.3 1.5 1.6 1.8 1.2 2.0 1.4 1.7 1.7
Intensity Change (Log2) -0.1 0.15 -0.43 0.03 -0.14 -0.07 -0.13 -0.13 0.2 0.22 0.19 0.34 0.35 -0.03 0.05 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.8 0.5 -3.2 -1.1 -3.2 -1.7 -2.2 -1.8 4.1 0.7 -1.1 0 0
Bud 0 0 2.2 0 0 0 0 0 2.9 3.3 3.4 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 -1.3 -4.6 -4.4 -7.1 -6.3 -6.7 -6.8 -4.7 -6.4 -3.6 0.1 0.3
Endoplasmic Reticulum -2.4 -1.9 -7.5 -3.2 -6.2 -6.9 -7.4 -5.1 -3.2 -8.5 -6.6 -3.2 -3.5
Endosome 0 2.0 0 0 0 0 0 0 2.6 7.1 5.9 0 0
Golgi 0 2.4 0 2.6 0 0.6 0 1.2 6.0 2.7 1.1 0 0
Mitochondria 5.5 3.2 11.3 5.0 12.2 11.5 12.8 12.1 3.3 3.1 3.3 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 7.4 5.5 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 4.5 3.5 0 0
Vacuole 0 0.3 2.7 5.0 3.9 2.8 2.9 2.0 0 0 0.6 0 0