Standard name
Description Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0 0 0.05 0 0.06 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0.06 0.10 0.11 0 0.07 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.21 0.08 0.22 0.09 0.19 0.15 0.09 0.22 0.21 0.17 0.11 0.07 0.10 0.19 0.09 0.11 0 0 0 0 0 0
Nucleus 0.10 0.11 0.13 0.07 0.07 0.06 0.05 0.07 0.05 0.08 0.06 0.09 0 0.07 0.17 0.17 0.14 0 0.09 0.07 0.07 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.94 0.92 0.90 0.95 0.90 0.89 0.90 0.87 0.87 0.84 0.85 0.87 0.85 0.77 0.82 0.80 0.84 0.89 0.84 0.82 0.83 0.80 0.75
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.08 0.06 0.15 0.16 0.23 0.21 0.25 0.25 0.29 0 0.05 0.07 0 0.06 0 0 0 0.05 0 0.06 0.12
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 1
Bud 0 0 0 0 4 7 2 12 8 6 2 1 0 0 2 0 1 1 4 1 3 7 5
Bud Neck 0 2 6 0 7 9 8 8 5 5 8 9 9 4 1 2 2 0 3 2 4 2 6
Bud Site 0 0 2 2 7 26 14 24 28 10 22 0 0 10 1 1 4
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
Cytoplasm 3 6 7 4 10 9 9 15 6 6 10 28 50 62 18 44 33 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 4 4 0 0 0 0 0 0
Endosome 0 2 2 0 15 9 17 19 1 1 3 6 4 12 1 7 8 0 1 6 2 9 10
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 2 2 2
Mitochondria 30 97 31 68 49 109 108 97 118 60 64 48 38 57 84 56 68 1 3 2 0 4 6
Nucleus 25 53 54 23 39 35 34 85 26 23 24 41 23 41 75 109 83 11 40 28 23 30 14
Nuclear Periphery 0 1 1 0 1 2 2 2 0 2 2 0 0 0 2 1 0 0 0 0 0 2 4
Nucleolus 240 427 370 292 507 500 658 993 470 246 316 380 439 426 361 519 514 226 393 347 293 463 427
Peroxisomes 2 5 2 0 7 7 16 41 11 3 6 2 2 13 1 6 4 0 1 0 1 1 4
SpindlePole 6 4 31 20 86 88 170 237 137 74 106 14 27 39 5 36 27 7 14 22 13 36 71
Vac/Vac Membrane 0 1 1 0 2 1 1 7 2 1 1 3 2 1 8 12 11 0 3 6 5 12 13
Unique Cell Count 254 464 409 308 562 562 734 1141 541 292 370 439 514 551 440 649 613 255 469 426 354 582 573
Labelled Cell Count 306 598 507 409 734 802 1039 1540 812 437 564 532 594 666 565 798 759 255 469 426 354 582 573

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Nucleolus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.9 24.0 15.5 14.3 13.2 10.2 9.9 9.6 8.9 8.1 8.0 18.3 16.4 14.6 21.7 26.3 23.8 21.2 20.0 20.4
Std Deviation (1e-4) 8.1 7.6 5.3 4.8 4.3 3.3 2.9 3.0 3.4 2.9 2.7 8.8 8.4 7.4 11.2 15.7 12.2 9.2 8.7 6.5
Intensity Change (Log2) -0.11 -0.23 -0.6 -0.64 -0.69 -0.79 -0.94 -0.94 0.24 0.08 -0.09 0.49 0.77 0.62 0.45 0.37 0.4

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 -0.3 0.2 -0.6 0 0 0 0.7 0.6 0.3 0 0 0 0
Bud Site 0 0 3.8 2.0 2.2 4.1 0 4.4 0 0 1.8 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.6 0 2.3 1.8 0 0 0 0 0 2.2 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.6 0.6 5.2 3.5 0.6 6.0 5.0 4.1 1.7 -0.1 1.5 4.9 0.6 1.9
Nucleus -2.2 -2.9 -3.9 -5.4 -5.0 -5.0 -2.5 -3.6 -1.7 -5.0 -2.8 0.5 0.7 -0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.2 -0.1 -0.8 -0.4 -1.8 -1.7 -2.5 -2.2 -1.8 -2.3 -5.4 -3.5 -4.5 -3.0
Peroxisomes 0 0 0 2.2 3.3 2.0 0 0 0 0 2.3 0 0 0
SpindlePole -0.6 3.7 3.8 6.6 6.1 7.1 6.5 7.7 -2.8 -1.4 -0.3 -4.7 -1.3 -2.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0