Standard name
Human Ortholog
Description E3 ubiquitin ligase targeting centromere-binding protein Cse4p; mediates polyubiquitination and degradation of histone H3 variant Cse4p, preventing its mislocalization to euchromatin independent of Slx5p; ubiquitination of Cse4p may be antagonized by Scm3p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0 0.05 0 0.06 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.07 0.05 0 0 0.05 0 0 0 0.12 0.16 0.15 0.13 0.09 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.16 0 0.17 0.08 0.25 0.32 0.18 0.23 0.44 0.41 0.41 0 0 0 0 0 0.07 0 0 0 0 0 0
Nucleus 0.94 0.93 0.95 0.76 0.86 0.84 0.84 0.84 0.81 0.75 0.74 0.77 0.86 0.82 0.82 0.8 0.79 0.82 0.9 0.92 0.9 0.88 0.75 0.65
Nuclear Periphery 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.18 0 0.06 0.08 0.08 0.07 0.1 0.14 0.14 0 0 0 0 0 0 0 0 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0 0.05 0 0.05 0.13 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 1 2 1 2 4 0 1 2 0 0 0 0 2 2 2 0 1 4 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 1 0 0 0 11 6 19 7 19 6 7 0 0 0 1 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 4 1 11 19 12 9 11 13 5 12 10 32 75 73 17 16 10 1 0 0 4 5 4
Endoplasmic Reticulum 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 3 7 4 0 0 0 0 0 1
Endosome 0 0 0 0 1 1 0 0 0 0 0 0 0 1 3 1 2 1 3 0 2 1 9 4
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 2 0 1 1
Mitochondria 54 30 7 33 21 60 107 64 61 141 115 115 8 1 3 4 7 9 6 0 0 0 5 8
Nucleus 305 175 177 152 219 200 280 293 211 240 206 214 226 376 399 107 147 103 291 178 178 218 278 305
Nuclear Periphery 2 1 5 3 6 7 11 18 8 4 10 6 2 7 6 3 8 2 0 0 0 0 0 3
Nucleolus 1 3 1 35 8 15 27 27 18 31 40 38 0 0 2 2 7 2 0 1 0 1 14 27
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 0 2 0 3 0 3 0 0 1 2 3 1 1 0 2 0 2 2 2 7
Vac/Vac Membrane 3 8 3 2 6 5 2 8 6 4 2 3 1 6 6 6 18 8 10 9 5 13 46 94
Unique Cell Count 323 189 187 199 254 237 335 348 262 322 278 278 263 456 484 134 185 126 323 194 197 248 373 469
Labelled Cell Count 370 222 198 238 281 314 444 444 326 451 391 394 273 468 496 145 214 141 323 194 197 248 373 469


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.5 5.9 5.5 5.9 4.9 4.7 4.9 4.9 4.4 4.3 4.2 6.4 6.9 6.6 9.0 8.5 8.2 7.5 7.6 7.8
Std Deviation (1e-4) 0.7 1.2 1.8 2.0 3.1 2.1 1.6 1.6 2.1 1.2 1.6 1.2 1.6 1.5 1.5 2.7 2.2 2.4 1.7 1.5 1.4
Intensity Change (Log2) – – – -0.1 -0.01 -0.27 -0.32 -0.28 -0.28 -0.45 -0.47 -0.5 0.1 0.21 0.15 0.6 0.52 0.47 0.33 0.35 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 3.3 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.8 3.5 2.7 0 0 2.7 0 2.4 0 4.7 5.7 5.4 4.7 3.7 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.1 1.9 6.0 0 4.8 5.7 0 0 0 -0.4 0 0 0 0 1.3
Nucleus -5.1 -2.9 -3.3 -3.7 -3.5 -4.3 -5.7 -5.7 -5.1 -3.0 -4.0 -4.1 -4.1 -4.4 -3.6
Nuclear Periphery 0 0 0.2 0 1.4 0.2 0 0 0 0 0 0 0 0.9 0
Nucleolus 5.8 0 3.1 0 3.6 3.3 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 3.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0667 3.4289 3.2609 2.6922 2.192 2.8783 2.2955 2.4074 2.1372 1.735 1.0545 2.028 1.2529 1.6876 1.3951 1.0891 1.0289 1.1891
Actin 0.0182 0 0.0037 0 0.0124 0.0023 0.0372 0.0019 0.0036 0.0162 0 0.0263 0.0514 0 0.0239 0 0.0017 0.0076
Bud 0.0001 0 0.0002 0 0.0004 0 0.0009 0.0001 0.0009 0.0011 0 0.0006 0.0002 0 0.0004 0.0001 0.0001 0.0001
Bud Neck 0.0005 0.0001 0.0004 0 0.0007 0.0007 0.0012 0.0002 0.0004 0.0004 0.0002 0.0015 0.0013 0 0.0095 0.0002 0.0011 0.0031
Bud Periphery 0.0003 0 0.0005 0 0.0015 0.0001 0.0015 0.0001 0.0007 0.0013 0 0.0022 0.0005 0 0.0007 0.0001 0.0001 0.0001
Bud Site 0.0005 0 0.0009 0 0.0014 0.0001 0.002 0.0016 0.0055 0.0018 0 0.0008 0.0009 0 0.0031 0 0.0003 0.0001
Cell Periphery 0.0001 0 0.0002 0 0.0004 0 0.0002 0 0.0002 0.0001 0 0.0003 0.0002 0 0.0006 0 0 0
Cytoplasm 0.0053 0 0.0006 0.0001 0.0003 0.0003 0.0429 0.0011 0.0042 0.0009 0.0001 0.0014 0.0012 0.0002 0.0008 0.0001 0.0007 0.0001
Cytoplasmic Foci 0.0055 0 0.0005 0 0.0039 0.0002 0.0361 0.0061 0.0058 0.0141 0 0.001 0.0042 0 0.0035 0 0.0001 0.0001
Eisosomes 0.0013 0 0.0002 0 0.0003 0.0001 0.0003 0 0 0.0001 0 0.0001 0.0005 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0016 0 0.0008 0 0.0018 0.0005 0.0106 0.0002 0.0023 0.0008 0 0.0049 0.0029 0 0.0006 0 0.0008 0.0002
Endosome 0.007 0 0.0028 0 0.0119 0.002 0.0404 0.0058 0.0146 0.0148 0 0.0063 0.0062 0 0.0058 0 0.0012 0.0009
Golgi 0.0029 0 0.0007 0 0.0056 0.0008 0.0148 0.0019 0.0059 0.0186 0 0.0032 0.0039 0 0.0049 0 0.0001 0.0007
Lipid Particles 0.009 0 0.0018 0 0.01 0.002 0.0161 0.0014 0.0027 0.0241 0 0.0059 0.0019 0 0.0099 0 0.0001 0.0004
Mitochondria 0.0016 0.0001 0.0055 0 0.0116 0.0015 0.0134 0.0009 0.0226 0.0137 0.0003 0.0272 0.0062 0.0002 0.0021 0.0004 0.001 0.0003
None 0.0046 0 0.0057 0 0.0008 0.0003 0.0071 0.0001 0.0003 0.0002 0 0.0003 0.0027 0 0.0004 0 0.0003 0.0001
Nuclear Periphery 0.0065 0.0033 0.0027 0.0004 0.0241 0.0086 0.0698 0.0036 0.0017 0.0053 0.0017 0.003 0.0044 0.0025 0.0047 0.0026 0.0202 0.0014
Nucleolus 0.0101 0.0087 0.0127 0.0056 0.0183 0.022 0.009 0.006 0.0052 0.0032 0.0148 0.0236 0.0068 0.0078 0.0159 0.0531 0.0336 0.0184
Nucleus 0.9078 0.9871 0.9535 0.9938 0.8577 0.9481 0.6384 0.963 0.9162 0.8718 0.9824 0.8863 0.8983 0.9866 0.9 0.9426 0.934 0.9652
Peroxisomes 0.0079 0 0.0008 0 0.021 0.0002 0.0333 0.0018 0.0013 0.0039 0 0.0012 0.0027 0 0.0036 0 0.0001 0.0002
Punctate Nuclear 0.006 0.0006 0.0018 0.0001 0.0082 0.0095 0.0098 0.0031 0.0007 0.0065 0.0002 0.0004 0.0025 0.0026 0.0068 0.0002 0.0038 0.0007
Vacuole 0.0023 0 0.002 0 0.0031 0.0003 0.0061 0.0006 0.0033 0.0005 0.0001 0.0018 0.0004 0.0001 0.0019 0.0002 0.0004 0.0001
Vacuole Periphery 0.0008 0 0.0018 0 0.0047 0.0004 0.0091 0.0004 0.0018 0.0006 0.0001 0.0016 0.0008 0.0001 0.0006 0.0002 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.8443 4.0392 9.5913 8.5248 11.1309 8.1287 6.1261 7.1668 8.9209 10.5218
Translational Efficiency 1.6266 3.3304 1.2015 1.6701 1.4226 2.2345 1.9979 1.3547 1.2443 1.5792

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description E3 ubiquitin ligase targeting centromere-binding protein Cse4p; mediates polyubiquitination and degradation of histone H3 variant Cse4p, preventing its mislocalization to euchromatin independent of Slx5p; ubiquitination of Cse4p may be antagonized by Scm3p
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Psh1

Psh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Psh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available