Standard name
Description E3 ubiquitin ligase targeting centromere-binding protein Cse4p; mediates polyubiquitination and degradation of histone H3 variant Cse4p, preventing its mislocalization to euchromatin independent of Slx5p; ubiquitination of Cse4p may be antagonized by Scm3p

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0 0.05 0 0.06 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.07 0.05 0 0 0.05 0 0 0 0.12 0.16 0.15 0.13 0.09 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.16 0 0.17 0.08 0.25 0.32 0.18 0.23 0.44 0.41 0.41 0 0 0 0 0 0.07 0 0 0 0 0 0
Nucleus 0.94 0.93 0.95 0.76 0.86 0.84 0.84 0.84 0.81 0.75 0.74 0.77 0.86 0.82 0.82 0.80 0.79 0.82 0.90 0.92 0.90 0.88 0.75 0.65
Nuclear Periphery 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.18 0 0.06 0.08 0.08 0.07 0.10 0.14 0.14 0 0 0 0 0 0 0 0 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.10 0.06 0 0.05 0 0.05 0.13 0.20
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 1 2 1 2 4 0 1 2 0 0 0 0 2 2 2 0 1 4 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 1 0 0 0 11 6 19 7 19 6 7 0 0 0 1 1 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 4 1 11 19 12 9 11 13 5 12 10 32 75 73 17 16 10 1 0 0 4 5 4
Endoplasmic Reticulum 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 3 7 4 0 0 0 0 0 1
Endosome 0 0 0 0 1 1 0 0 0 0 0 0 0 1 3 1 2 1 3 0 2 1 9 4
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 2 0 1 1
Mitochondria 54 30 7 33 21 60 107 64 61 141 115 115 8 1 3 4 7 9 6 0 0 0 5 8
Nucleus 305 175 177 152 219 200 280 293 211 240 206 214 226 376 399 107 147 103 291 178 178 218 278 305
Nuclear Periphery 2 1 5 3 6 7 11 18 8 4 10 6 2 7 6 3 8 2 0 0 0 0 0 3
Nucleolus 1 3 1 35 8 15 27 27 18 31 40 38 0 0 2 2 7 2 0 1 0 1 14 27
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 0 2 0 3 0 3 0 0 1 2 3 1 1 0 2 0 2 2 2 7
Vac/Vac Membrane 3 8 3 2 6 5 2 8 6 4 2 3 1 6 6 6 18 8 10 9 5 13 46 94
Unique Cell Count 323 189 187 199 254 237 335 348 262 322 278 278 263 456 484 134 185 126 323 194 197 248 373 469
Labelled Cell Count 370 222 198 238 281 314 444 444 326 451 391 394 273 468 496 145 214 141 323 194 197 248 373 469

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.5 5.9 5.5 5.9 4.9 4.7 4.9 4.9 4.4 4.3 4.2 6.4 6.9 6.6 9.0 8.5 8.2 7.5 7.6 7.8
Std Deviation (1e-4) 0.7 1.2 1.8 2.0 3.1 2.1 1.6 1.6 2.1 1.2 1.6 1.2 1.6 1.5 1.5 2.7 2.2 2.4 1.7 1.5 1.4
Intensity Change (Log2) -0.1 -0.0 -0.3 -0.3 -0.3 -0.3 -0.4 -0.5 -0.5 0.1 0.2 0.1 0.6 0.5 0.5 0.3 0.4 0.4

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 3.3 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.1 1.9 6.0 0 4.8 5.7 0 0 0 -0.4 0 0 0 0 1.3
Nucleus 0 1.3 3.0 0 6.9 2.9 0 0 0 0 0 0 0 5.4 0
Nuclear Periphery 0 0 0.2 0 1.4 0.2 0 0 0 0 0 0 0 0.9 0
Nucleolus 5.8 0 3.1 0 3.6 3.3 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 2.7 0