Standard name
Human Ortholog
Description Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.89 0.86 0.43 0.38 0.47 0.43 0.4 0.34 0.45 0.37 0.41 0.77 0.86 0.95 0.35 0.32 0.38 0.76 0.58 0.77 0.8 0.76 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.14 0.41 0.35 0.59 0.47 0.58 0.46 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0.1 0.16 0.36 0.24 0.25 0.32 0.26 0.25 0.26 0.23 0.1 0.05 0 0.17 0.07 0.08 0.11 0.22 0.09 0.07 0.07 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.1 0 0.44 0.3 0.25 0.13 0.1 0.09 0.08 0.1 0.11 0.26 0.18 0.07 0.59 0.73 0.63 0.05 0.09 0.09 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 1 1
Bud 0 0 1 2 0 1 5 2 3 7 7 4 0 0 0 0 0 0 2 2 1 2 9 11
Bud Neck 0 0 0 0 0 1 0 1 0 1 0 1 0 0 0 0 0 0 1 1 0 0 4 6
Bud Site 0 0 0 0 0 0 0 0 1 2 3 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 1 0 0 1 1 0 1 1 0 3 1 0 0 0 0 0 0 0 0 0
Cytoplasm 299 91 62 61 80 107 99 106 47 93 129 137 257 523 527 51 59 60 273 73 88 227 361 441
Endoplasmic Reticulum 3 3 1 0 0 0 0 1 0 0 0 0 6 0 2 8 9 6 1 2 1 3 3 6
Endosome 0 0 0 4 2 1 0 1 0 0 0 0 1 3 0 2 2 1 3 2 0 3 10 11
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1 5 1 1 1 0 1 3 3
Mitochondria 11 2 0 8 9 33 94 91 83 97 204 153 1 0 0 2 1 4 4 2 0 2 1 6
Nucleus 11 3 7 23 75 55 57 83 36 51 91 76 32 28 10 25 13 13 39 27 9 18 33 14
Nuclear Periphery 0 0 0 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 1 0 0 0 0 0 0 2 0 0 0 0 0 1 0 0 0 0 0 2 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 0 0 5 1 0 4 11 9
Vac/Vac Membrane 40 10 1 62 63 58 31 26 13 16 36 35 87 108 41 85 134 101 17 11 9 12 23 24
Unique Cell Count 333 102 72 142 210 229 231 263 140 208 351 331 335 608 557 144 184 160 359 127 115 284 476 547
Labelled Cell Count 365 110 72 161 230 257 288 313 184 268 474 409 385 665 581 175 224 186 359 127 115 284 476 547


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 7.3 5.7 6.3 6.4 5.2 4.7 4.6 3.9 4.0 3.8 4.0 7.7 7.6 7.3 10.3 12.1 12.0 7.6 7.8 8.2
Std Deviation (1e-4) 1.0 1.1 0.6 1.3 1.6 1.7 1.9 1.0 1.2 1.1 1.1 0.8 1.2 1.1 1.2 1.8 2.0 2.3 1.6 1.3 1.8
Intensity Change (Log2) – – – 0.15 0.16 -0.12 -0.28 -0.31 -0.56 -0.52 -0.6 -0.52 0.43 0.42 0.36 0.86 1.09 1.07 0.42 0.46 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -6.0 -7.0 -5.9 -6.4 -6.9 -7.2 -6.1 -7.7 -6.9 -1.8 0 2.8 -7.0 -7.8 -6.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 1.3 4.2 2.6 2.7 3.7 2.7 2.7 3.0 2.5 0 0 0 1.5 -0.7 -0.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 6.4 5.0 4.5 2.9 2.3 0 0 2.4 2.5 4.6 3.6 0 8.2 10.3 8.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0278 1.628 1.4915 1.3895 0.7814 1.3058 1.6454 2.3623 2.0191 2.1923 1.973 2.4328 1.7075 2.385 1.8811 1.8276 1.7384 1.7035
Actin 0.0281 0.0005 0.0094 0.0063 0.0067 0.0061 0.0077 0.0002 0.0082 0.0048 0.0442 0.0057 0.0043 0.0004 0.0004 0.0007 0.0049 0.0003
Bud 0.0009 0.0061 0.005 0.0012 0.0009 0.0002 0.0009 0.0002 0.0008 0.002 0.0003 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001
Bud Neck 0.0097 0.0009 0.0074 0.003 0.0014 0.0018 0.0019 0.0003 0.0016 0.0024 0.0016 0.0025 0.0005 0.0013 0.0007 0.0007 0.0016 0.0021
Bud Periphery 0.0011 0.0012 0.0019 0.0007 0.0022 0.0002 0.0008 0.0001 0.0005 0.0015 0.0004 0.0001 0.0002 0 0.0001 0 0.0001 0
Bud Site 0.008 0.0114 0.0072 0.0022 0.001 0.0002 0.0028 0.0022 0.0051 0.0025 0.0008 0.0002 0.0007 0.0019 0.0004 0.0004 0.0003 0.0001
Cell Periphery 0.0002 0.0001 0.0001 0 0.0001 0 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0 0 0
Cytoplasm 0.539 0.672 0.5503 0.5006 0.593 0.628 0.6603 0.7612 0.658 0.6243 0.6651 0.6911 0.6808 0.7428 0.7189 0.5783 0.7244 0.7131
Cytoplasmic Foci 0.0216 0.0086 0.0131 0.0204 0.0171 0.0133 0.0183 0.0052 0.0104 0.0256 0.0139 0.0078 0.0061 0.0066 0.0036 0.0274 0.0054 0.0114
Eisosomes 0.0004 0 0.0002 0 0.0001 0 0.0001 0 0.0002 0.0001 0.0008 0.0001 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0064 0.0056 0.0046 0.0043 0.0059 0.0034 0.006 0.0027 0.006 0.0027 0.0054 0.0034 0.0069 0.0035 0.0059 0.0044 0.0071 0.0048
Endosome 0.0341 0.0078 0.019 0.0205 0.0227 0.0148 0.0181 0.0019 0.0081 0.0098 0.0177 0.0069 0.0105 0.005 0.0052 0.0154 0.0201 0.0092
Golgi 0.0076 0.0003 0.0041 0.0051 0.0094 0.003 0.0028 0.0001 0.0021 0.0049 0.0068 0.0006 0.0012 0.0002 0.0001 0.0007 0.0024 0.0002
Lipid Particles 0.0085 0.0002 0.0045 0.0116 0.0124 0.0034 0.0131 0.0001 0.0025 0.0052 0.009 0.0009 0.0012 0.0003 0.0002 0.001 0.0066 0.0001
Mitochondria 0.0103 0.0007 0.0056 0.0066 0.0107 0.0028 0.0018 0.0002 0.0031 0.001 0.0059 0.0005 0.0007 0.0002 0.0004 0.0003 0.002 0.0003
None 0.1622 0.0793 0.1417 0.0206 0.1326 0.1424 0.0948 0.1289 0.1006 0.1009 0.0415 0.0559 0.0684 0.037 0.0515 0.0616 0.0136 0.0626
Nuclear Periphery 0.0167 0.0111 0.015 0.0155 0.0169 0.011 0.01 0.0041 0.0113 0.0074 0.0112 0.0073 0.0124 0.0107 0.0159 0.0136 0.021 0.0085
Nucleolus 0.0023 0.0014 0.0013 0.0015 0.0066 0.0006 0.003 0.0003 0.0006 0.0007 0.0011 0.0006 0.0005 0.003 0.0004 0.0005 0.0032 0.0003
Nucleus 0.1121 0.1751 0.184 0.3127 0.1146 0.1471 0.1218 0.0851 0.1592 0.1806 0.1312 0.1993 0.1967 0.1778 0.1888 0.2441 0.1696 0.1794
Peroxisomes 0.0105 0.0003 0.0031 0.033 0.0176 0.0016 0.0126 0.0001 0.0034 0.0062 0.0029 0.0003 0.0006 0.0001 0.0001 0.001 0.0016 0.0003
Punctate Nuclear 0.013 0.0138 0.018 0.0317 0.022 0.0175 0.0178 0.0058 0.0148 0.0148 0.0368 0.0139 0.0042 0.0035 0.0044 0.0479 0.0117 0.0053
Vacuole 0.0058 0.0032 0.0034 0.002 0.0037 0.0018 0.0044 0.0013 0.0027 0.0021 0.0021 0.0023 0.0035 0.005 0.0024 0.0013 0.0029 0.0016
Vacuole Periphery 0.0015 0.0005 0.001 0.0006 0.0024 0.0006 0.0009 0.0002 0.0006 0.0005 0.001 0.0004 0.0004 0.0003 0.0004 0.0004 0.0013 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.2274 15.7066 17.871 16.0483 22.7804 26.926 16.1456 22.509 22.753 17.1877
Translational Efficiency 2.532 2.7361 2.2027 2.5261 1.6033 1.3788 1.9595 1.4503 1.3214 1.9304

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘