Standard name
Description Beta' regulatory subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0 0.09 – 0.07 0.14 0.09 0.08 0 0.09 0 0 0.06 0.12 0.2 0.08 0.12 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0.1 0 0.13 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.67 0.5 0.77 – 0.77 0.72 0.73 0.7 0.67 0.63 0.6 0.63 0.64 0.74 0.62 0.76 0.67 0.5 0.76 0.75 0.73 0.78 0.52 0.41
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0.16 0 – 0 0.05 0.05 0.05 0.11 0.1 0.22 0.09 0.11 0 0 0 0 0 0.05 0 0.07 0 0.09 0.11
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.22 0.5 0.2 – 0.13 0.11 0.16 0.21 0.19 0.17 0.13 0.2 0.21 0.2 0.18 0.14 0.19 0.37 0.1 0.13 0.1 0.08 0.25 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 1 1 2 2 4 0 0 0 0 0 0 3 1 1 1 7 5
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 2 3 1 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 1 4 – 6 27 22 24 8 22 2 7 5 26 39 4 8 10 2 1 2 2 4 7
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 0 0 1 2 2
Endosome 0 0 0 – 0 0 3 2 0 4 2 2 0 1 7 0 2 0 0 0 1 0 3 11
Golgi 0 0 1 – 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 1 0 2 1
Mitochondria 0 1 0 – 0 2 2 2 18 10 19 20 1 0 0 1 1 0 0 0 0 1 1 2
Nucleus 37 19 34 – 63 136 174 209 123 148 90 129 52 161 124 37 46 35 72 69 68 86 117 107
Nuclear Periphery 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 3 6 0 – 3 9 12 14 20 23 33 19 9 2 4 2 2 3 5 3 6 4 21 28
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 1 0 0 1 1 0 0 1 0 0 0 0 0 0 1 3 11
Vac/Vac Membrane 12 19 9 – 11 20 39 63 35 39 20 42 17 43 35 7 13 26 9 11 9 8 56 75
Unique Cell Count 55 38 44 82 189 237 298 184 236 149 205 81 218 199 49 69 70 96 92 94 111 224 262
Labelled Cell Count 58 46 48 83 194 252 317 207 251 170 232 85 233 212 53 74 75 96 92 94 111 224 262


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.8 9.8 12.5 – 12.3 11.2 10.5 10.5 9.4 9.8 9.2 9.7 11.1 11.9 11.5 12.0 14.4 15.7 13.4 13.3 13.3
Std Deviation (1e-4) 2.5 1.9 1.9 – 2.0 2.4 1.7 1.9 1.8 1.9 1.9 2.1 2.7 2.4 2.7 3.0 3.8 4.6 2.3 2.0 2.3
Intensity Change (Log2) – – – – -0.02 -0.16 -0.25 -0.25 -0.42 -0.36 -0.45 -0.38 -0.17 -0.08 -0.13 -0.06 0.2 0.33 0.09 0.08 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0.9 0 0 0 0 0 0 0 0.5 1.7 0 0 0.8
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – -0.1 -0.7 -0.5 -1.0 -1.3 -1.9 -2.1 -1.8 -1.5 -0.5 -1.9 -0.2 -1.2 -2.9
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 3.4 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole – -1.0 -1.8 -0.6 0.1 -0.2 -0.6 -1.1 0 0.1 -0.1 -0.4 -0.8 -0.2 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.7625 8.2679 6.9455 5.4043 5.7535 6.8841 7.8963 10.2625 9.0932 8.4488 8.4199 9.0135 5.4316 7.0404 7.1716 7.3605 6.2348 6.4238
Actin 0.0112 0 0.0043 0 0.0004 0.0015 0.0469 0.0138 0.0074 0.0033 0.0002 0.0018 0.0387 0 0.0074 0.0001 0 0.0001
Bud 0.0003 0 0.0003 0.0001 0.0001 0.0001 0.0004 0.0001 0.0007 0.0002 0.0001 0.0001 0.0004 0.0002 0.0002 0.0001 0 0.0002
Bud Neck 0.0007 0.0006 0.0013 0.0011 0.0016 0.0087 0.0014 0.001 0.002 0.0026 0.0013 0.0107 0.0019 0.0007 0.0007 0.0001 0.0003 0.0046
Bud Periphery 0.0004 0 0.0006 0.0001 0.0001 0.0004 0.0006 0.0002 0.0014 0.0004 0.0001 0.0003 0.0005 0.0001 0.0003 0.0001 0 0.0003
Bud Site 0.0007 0.0003 0.0008 0.0002 0.001 0.0005 0.0039 0.0006 0.0066 0.0009 0.0002 0.0003 0.0033 0.0019 0.0031 0.0001 0 0.0002
Cell Periphery 0.0001 0.0001 0.0004 0 0.0001 0.0002 0.0008 0.0004 0.0005 0.0001 0.0001 0.0002 0.0002 0.0002 0.0001 0 0 0.0001
Cytoplasm 0.0012 0.0006 0.0009 0.0004 0.0016 0.0004 0.0182 0.0009 0.0016 0.0009 0.0014 0.0009 0.0043 0.0036 0.0004 0.0009 0.0012 0.0007
Cytoplasmic Foci 0.0061 0 0.0014 0 0.0129 0.0006 0.0151 0.0004 0.0054 0.0098 0.0004 0.0019 0.024 0 0.0002 0 0 0
Eisosomes 0 0 0 0 0 0 0.0005 0.0001 0 0 0 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0005 0 0.0014 0.0001 0.0005 0.0001 0.0039 0.0004 0.0013 0.0006 0.0011 0.0004 0.0022 0.0004 0.0001 0.0001 0 0.0001
Endosome 0.0051 0 0.0064 0.0001 0.0145 0.0011 0.0243 0.0013 0.0109 0.0081 0.0024 0.0006 0.0059 0.0002 0.0003 0 0 0.0003
Golgi 0.0026 0 0.0028 0 0.0019 0.0007 0.006 0.0012 0.0024 0.0031 0.0002 0.0004 0.0025 0 0.0006 0 0 0
Lipid Particles 0.0024 0 0.0032 0 0.0081 0.0018 0.0098 0.0008 0.0165 0.0049 0.0013 0.0009 0.0042 0 0.0001 0 0 0
Mitochondria 0.001 0.0002 0.0016 0.0003 0.0004 0.0003 0.0036 0.0003 0.0042 0.0006 0.0003 0.0002 0.0029 0.0002 0.0037 0.0003 0.0001 0.0005
None 0.0006 0 0.0002 0 0.0008 0.0001 0.0218 0.0002 0.0002 0.0005 0.0002 0.0001 0.0008 0 0 0 0 0.0001
Nuclear Periphery 0.0045 0.0032 0.0032 0.0095 0.0058 0.0026 0.0231 0.0023 0.0062 0.0115 0.0516 0.0019 0.0182 0.0072 0.0035 0.0078 0.0021 0.0029
Nucleolus 0.0315 0.0431 0.0285 0.0235 0.0535 0.0523 0.03 0.0165 0.0316 0.0287 0.0156 0.0347 0.0214 0.0228 0.0339 0.0018 0.0017 0.0265
Nucleus 0.9247 0.9507 0.9358 0.9628 0.8799 0.9235 0.7517 0.9566 0.8852 0.9102 0.9163 0.9355 0.8531 0.9586 0.9444 0.9878 0.994 0.9607
Peroxisomes 0.0028 0 0.0004 0 0.0016 0.0012 0.0091 0.0002 0.0056 0.0016 0 0.0025 0.0046 0 0.0002 0 0 0
Punctate Nuclear 0.0027 0.0006 0.0011 0.0009 0.0139 0.0023 0.0213 0.0008 0.0014 0.0111 0.0055 0.0051 0.0057 0.0005 0.0003 0.0001 0.0002 0.0007
Vacuole 0.0007 0.0004 0.0048 0.0006 0.0011 0.0014 0.0062 0.0016 0.0074 0.0008 0.0009 0.0013 0.0039 0.0031 0.0004 0.0004 0.0002 0.0011
Vacuole Periphery 0.0002 0.0001 0.0005 0.0003 0.0003 0.0002 0.0013 0.0001 0.0017 0.0003 0.0009 0.0001 0.0012 0.0002 0.0001 0.0003 0 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 73.1343 63.4734 49.926 56.455 43.2682 76.642 56.7974 54.1447 59.4655 71.9503
Translational Efficiency 1.4991 1.1772 1.2105 1.1689 1.9779 1.4112 1.2787 1.3112 1.2249 1.2609

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘