Standard name
Description AN1-type zinc finger protein, effector of proteotoxic stress response; stress-inducible transcriptional target of Rpn4p; induced by nitrogen limitation, weak acid, misfolded proteins; short-lived protein, degraded by proteasome; may protect cells from trivalent metalloid induced proteotoxicity; contains PACE promoter element; ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases under DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.05 0.27 0.08 0.09 0.12 0.09 0.19 0.17 0.24 0.2 0.27 0.08 0.17 0.27 0.18 0.18 0.14 0 0 0.13 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.05 0.63 0.42 0.09 0.54 0.61 0.62 0.81 0.71 0.8 0.76 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.83 0.78 0.33 0.74 0.83 0.67 0.57 0.41 0.28 0.27 0.28 0.2 0.85 0.7 0.56 0.55 0.55 0.63 0.78 0.81 0.54 0.74 0.72 0.65
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.17 0 0.07 0.06 0.09 0.11 0.08 0.1 0.07 0.06 0.07 0.11 0.18 0.27 0.3 0.34 0.3 0.13 0.14 0.23 0.13 0.14 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud 0 0 0 0 0 1 6 11 4 8 4 9 0 0 0 1 0 0 4 1 0 5 9 13
Bud Neck 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 4 3 2 8 10 4 5 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 27 3 14 12 20 31 44 98 56 116 96 156 36 82 138 23 18 17 16 1 10 9 13 7
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 4 3 6 0 0 0 0 0 2
Endosome 0 0 0 0 1 0 2 1 0 0 0 1 2 1 2 5 2 1 2 0 0 2 4 2
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 1
Mitochondria 53 3 32 64 20 140 306 322 269 340 393 444 15 9 6 4 2 3 1 0 2 1 6 3
Nucleus 308 51 17 112 191 174 287 211 95 128 139 117 381 332 284 71 55 76 323 67 43 159 295 276
Nuclear Periphery 0 0 0 1 0 4 7 6 4 1 4 4 0 0 0 0 0 1 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 3 7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 2 1 0 0 0 0 1 0 6 0 1 5 8 6
Vac/Vac Membrane 48 11 1 11 14 23 56 41 32 31 30 38 50 88 137 38 34 36 54 11 18 29 58 93
Unique Cell Count 372 65 51 151 230 261 500 519 334 476 490 583 447 477 510 128 100 121 414 84 81 216 409 426
Labelled Cell Count 436 69 64 200 247 377 712 694 469 638 673 775 485 512 568 146 115 140 414 84 81 216 409 426


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.0 3.3 4.4 5.4 4.1 3.6 3.5 2.9 3.2 2.9 2.9 5.6 5.8 5.6 8.9 9.2 8.9 5.2 6.1 6.4
Std Deviation (1e-4) 0.6 0.7 0.7 1.6 1.4 1.6 1.1 0.9 0.8 1.0 1.2 1.0 1.0 1.3 1.2 3.0 3.2 3.8 1.3 1.5 1.5
Intensity Change (Log2) – – – 0.42 0.72 0.33 0.12 0.08 -0.18 -0.06 -0.18 -0.2 0.76 0.83 0.78 1.45 1.49 1.44 0.66 0.9 0.96


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.6 -3.7 -2.9 -4.1 -1.5 -1.8 -0.5 -1.3 -0.1 -4.4 -1.8 -0.1 -1.4 -1.3 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -9.0 0 0 0 0 0 0 0 0 0 0 -9.0 -8.5 -9.0
Nucleus 5.2 7.3 4.5 3.3 1.0 -0.7 -1.0 -0.7 -2.2 8.8 5.2 3.1 2.7 2.5 3.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.1 0 0 0 0 0 2.1 3.0 3.9 4.1 4.4 4.1