Standard name
Description Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.11 0.12 0.37 0.21 0.32 0.39 0.47 0.58 0.33 0.41 0.32 0.37 0.18 0.34 0.39 – – 0.24 0.08 0.11 0.12 0.1 0.18 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Endosome 0 0 0 0.18 0.16 0.06 0.05 0 0 0 0 0 0.15 0 0.07 – – 0.14 0.06 0.06 0.05 0.06 0.08 0.16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.5 0.8 0 0.1 0 0.25 0.33 0.26 0.64 0.5 0.67 0.56 0 0 0 – – 0.09 0.05 0.08 0.11 0.06 0.1 0.11
Nucleus 0.61 0.22 0.41 0.34 0.23 0.25 0.13 0.08 0.09 0.1 0.06 0.1 0.48 0.49 0.39 – – 0.29 0.28 0.3 0.26 0.23 0.15 0.06
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0.05 0.05 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0.08 0.06 0.12 0.08 0.07 0.1 0.1 0.1 0.09 0.12 0.16 0.07 0.05 – – 0.19 0.19 0.1 0.13 0.28 0.21 0.28
Vac/Vac Membrane 0.06 0.18 0.17 0.17 0.3 0.12 0.07 0.05 0 0.06 0 0 0.1 0.07 0.17 – – 0.24 0.22 0.21 0.22 0.14 0.18 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – – 0 0 0 0 0 0 1
Bud 0 0 0 0 0 2 1 2 11 3 7 8 0 0 0 – – 0 1 0 1 0 1 5
Bud Neck 0 0 2 0 1 1 2 6 3 12 2 4 1 2 0 – – 0 0 0 0 0 1 4
Bud Site 0 0 0 0 0 0 2 0 2 6 5 10 1 0 0 – – 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 2 6 32 16 38 80 109 140 103 175 70 110 24 99 131 – – 39 5 9 19 10 44 26
Endoplasmic Reticulum 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 – – 6 0 0 0 0 0 0
Endosome 0 1 2 14 19 13 12 10 1 2 4 2 20 10 25 – – 23 4 4 8 6 18 53
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 2 0 0 0 1
Mitochondria 9 41 2 8 1 50 77 62 202 211 149 169 3 9 0 – – 15 3 6 18 5 24 37
Nucleus 11 11 36 26 28 51 29 20 29 44 14 31 63 143 130 – – 47 21 25 41 24 36 21
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 – – 0 1 0 2 0 0 1
Nucleolus 0 0 0 0 0 0 0 1 1 1 0 3 1 0 0 – – 6 2 3 3 5 11 16
Peroxisomes 0 0 0 1 0 1 3 1 1 1 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 2 7 5 14 17 16 25 31 42 21 37 21 21 16 – – 30 14 8 22 29 52 94
Vac/Vac Membrane 1 9 15 13 36 25 16 13 6 25 8 9 13 20 58 – – 38 16 17 36 14 45 54
Unique Cell Count 18 51 87 77 120 204 231 240 315 425 222 301 130 294 334 160 76 84 164 104 250 338
Labelled Cell Count 23 70 96 84 138 240 268 280 390 522 281 383 147 306 360 205 76 84 164 104 250 338


Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.5 5.4 5.5 5.6 4.4 4.3 4.7 3.7 3.8 4.0 4.0 5.3 5.4 5.0 3.9 4.8 4.9 6.0 5.7 5.6
Std Deviation (1e-4) 0.3 0.7 1.5 2.1 1.3 1.4 1.6 1.7 1.3 1.1 5.1 5.9 1.1 1.4 1.1 0.8 1.1 1.1 2.1 1.5 1.5
Intensity Change (Log2) – – – 0.03 0.05 -0.28 -0.32 -0.2 -0.56 -0.49 -0.44 -0.44 -0.02 -0.0 -0.11 -0.47 -0.16 -0.12 0.17 0.1 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3351 -0.0881 -0.3436 -0.6276 -0.6299 -0.2688 1.326 1.4415 1.1463 1.0717 1.0279 0.9272 -1.4963 -1.0416 -1.0553 -0.9739 -1.0917 -1.208
Actin 0.0016 0.0012 0.0007 0.0003 0.0025 0.0007 0.0546 0.0037 0.0211 0.0029 0.0069 0.0016 0.03 0.0014 0.0182 0.0037 0.0155 0.0007
Bud 0.0002 0.0002 0.0001 0 0.0002 0.0004 0.0007 0.001 0.0004 0.0001 0.0001 0.0002 0.0005 0.0006 0.0002 0.0002 0.003 0.0001
Bud Neck 0.0027 0.0015 0.0007 0.0004 0.0008 0.0093 0.0092 0.0012 0.0045 0.0008 0.0005 0.0064 0.0166 0.0013 0.0031 0.0008 0.0267 0.0062
Bud Periphery 0.0003 0.0003 0.0001 0 0.0002 0.0002 0.0015 0.0008 0.0009 0.0002 0.0002 0.0002 0.0007 0.0003 0.0002 0.0003 0.0084 0.0001
Bud Site 0.001 0.0021 0.0006 0.0001 0.0002 0.0002 0.0049 0.0053 0.0047 0.0004 0.0002 0.0004 0.0072 0.0035 0.004 0.0007 0.0075 0.0002
Cell Periphery 0.0001 0.0001 0.0001 0 0 0.0001 0.0006 0.0002 0.0005 0.0001 0.0001 0.0001 0.0004 0.0001 0.0002 0.0004 0.0002 0.0001
Cytoplasm 0.1146 0.1152 0.1194 0.108 0.0899 0.0686 0.1367 0.1671 0.125 0.1086 0.1243 0.1389 0.1903 0.2109 0.2282 0.1922 0.221 0.2022
Cytoplasmic Foci 0.0333 0.0148 0.015 0.0123 0.0273 0.016 0.0499 0.0084 0.0216 0.0046 0.0233 0.0203 0.0318 0.0177 0.0131 0.0052 0.0356 0.0168
Eisosomes 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0009 0.0001 0.0007 0.0002 0.0003 0.0001 0.0003 0.0001 0.0003 0.0001 0.0002 0
Endoplasmic Reticulum 0.0047 0.0065 0.0053 0.0053 0.0048 0.002 0.0101 0.0058 0.0069 0.0058 0.0052 0.0045 0.0087 0.011 0.0101 0.0063 0.007 0.0068
Endosome 0.017 0.0096 0.0137 0.004 0.0088 0.0041 0.021 0.0134 0.0114 0.0078 0.027 0.008 0.0204 0.0077 0.0129 0.0252 0.0204 0.0105
Golgi 0.001 0.0003 0.0004 0.0002 0.0003 0.0002 0.0099 0.0091 0.0036 0.0002 0.0106 0.0008 0.0049 0.0004 0.0008 0.0075 0.0032 0.0003
Lipid Particles 0.0048 0.0028 0.0019 0.0007 0.0023 0.0033 0.0153 0.0004 0.0074 0.0007 0.0166 0.0037 0.0096 0.0066 0.0026 0.0065 0.0031 0.0017
Mitochondria 0.001 0.0008 0.001 0.0006 0.001 0.0005 0.0031 0.0116 0.0037 0.0019 0.0081 0.0011 0.0047 0.0008 0.0012 0.0314 0.0097 0.0149
None 0.2993 0.2482 0.3636 0.393 0.3245 0.2424 0.2896 0.3698 0.3728 0.47 0.5134 0.2538 0.2976 0.2363 0.2048 0.3084 0.2319 0.1327
Nuclear Periphery 0.036 0.0325 0.0433 0.0171 0.0328 0.0161 0.0269 0.0178 0.0439 0.0318 0.0269 0.0127 0.0221 0.0383 0.0609 0.0433 0.0205 0.0374
Nucleolus 0.0116 0.0162 0.0062 0.0054 0.0098 0.0206 0.0122 0.0052 0.0072 0.0088 0.0018 0.0114 0.0048 0.0068 0.0043 0.0037 0.0049 0.0104
Nucleus 0.248 0.3978 0.3325 0.2095 0.3616 0.4191 0.1628 0.3196 0.2727 0.2905 0.1817 0.3896 0.1908 0.324 0.3693 0.308 0.2865 0.4562
Peroxisomes 0.0013 0.0005 0.001 0.0005 0.0014 0.0004 0.01 0.0004 0.0036 0.0006 0.0042 0.0057 0.0026 0.0005 0.0016 0.0011 0.0018 0.0006
Punctate Nuclear 0.218 0.1456 0.0911 0.2405 0.1286 0.1943 0.1763 0.0546 0.0838 0.057 0.0454 0.1385 0.148 0.1265 0.0581 0.0216 0.0859 0.099
Vacuole 0.0026 0.0029 0.0023 0.0015 0.0021 0.0011 0.0028 0.0035 0.0025 0.0059 0.0019 0.0017 0.007 0.0045 0.0048 0.0299 0.0042 0.0025
Vacuole Periphery 0.0007 0.0006 0.0007 0.0004 0.0007 0.0003 0.0009 0.0009 0.001 0.0012 0.0012 0.0003 0.001 0.0007 0.0013 0.0034 0.0026 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.8407 14.0183 14.1848 10.7584 9.2417 8.5488 8.6976 9.5664 10.8951 11.4445
Translational Efficiency 0.597 0.2313 0.2875 0.3504 0.285 0.5619 0.7432 0.7283 0.4379 0.3648

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘