Standard name
Description Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; plays an important role in function of eIF5B in stimulating 3' end processing of 18S rRNA in context of 80S ribosomes that have not yet engaged in translation; involved in replication and maintenance of killer double stranded RNA virus
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.07 0.07 0 0.08 0 0 0 0 – 0.09 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 1.0 1.0 0.98 0.94 0.89 0.76 0.75 0.8 0.68 0.73 1.0 0.99 0.99 – 1.0 0.98 0.98 0.97 0.95 0.92 0.95 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0.09 0.17 0.18 0.14 0.21 0.19 0 0 0 – 0.09 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 3 6 5 7 13 13 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 1 0 0 1 0 5 5 4 2 1 1 1 3 0 – 0 0 0 0 0 2 3 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 0 4 1 0 5 16 30 21 12 18 9 4 10 6 – 2 1 0 0 0 0 0 0
Cytoplasm 176 93 266 246 279 274 322 331 235 233 161 165 304 606 521 – 22 51 164 97 256 200 463 455
Endoplasmic Reticulum 0 0 0 0 6 12 32 76 57 42 50 42 2 5 2 – 2 5 0 0 6 0 2 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 1 0 0 0 1 5 6 2 10 7 0 1 2 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2 0 2 3 4 3 2 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 3 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 4 0 0 1 – 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 4 6 3
Unique Cell Count 176 93 267 246 285 292 363 435 313 292 237 225 304 613 524 22 52 168 101 269 219 489 475
Labelled Cell Count 177 94 271 247 286 295 379 455 331 303 256 243 311 626 532 27 57 168 101 269 219 489 475


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 141.6 214.7 176.1 156.5 122.1 89.9 76.7 75.6 69.7 64.9 63.4 62.1 186.3 176.5 161.6 – 174.5 213.3 164.7 171.3 186.3
Std Deviation (1e-4) 30.3 41.7 34.5 32.4 24.7 22.4 20.1 18.0 19.9 15.3 19.6 16.0 42.1 43.9 33.3 – 70.5 42.3 54.5 48.3 52.4
Intensity Change (Log2) – – – -0.17 -0.53 -0.97 -1.2 -1.22 -1.34 -1.44 -1.47 -1.5 0.08 0.0 -0.12 – -0.01 0.28 -0.1 -0.04 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 3.9 4.0 0 0 0 – 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 2.1 3.2 3.1 1.8 3.3 1.7 0 0 0 – 0 0
Cytoplasm 1.0 -1.8 -3.8 -5.4 -8.5 -8.6 -7.6 -9.9 -8.8 1.1 -1.1 -0.4 – 0.3 -1.3
Endoplasmic Reticulum 0 0 3.3 5.0 7.2 7.3 6.4 7.9 7.4 0 0 0 – 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 0 0 0 2.9 0 0 0 0 – 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 182.9226 221.5342 204.6941 179.113 202.1812 156.7346 207.89 269.1986 234.653 215.9984 212.8748 237.0373 222.6712 297.145 256.1603 234.6024 251.8804 213.262
Actin 0.0003 0.0004 0.0001 0.0001 0.0001 0.0005 0.0009 0.0002 0.0005 0.0002 0.0005 0.0002 0.0007 0.0001 0.0048 0.0005 0.0005 0.0015
Bud 0.0007 0.0002 0.0004 0.0005 0.0003 0.0006 0.001 0.0008 0.0019 0.0008 0.0025 0.0006 0.002 0.0002 0.0025 0.0015 0.0006 0.0008
Bud Neck 0.0004 0.0004 0.0005 0.0005 0.001 0.0013 0.0004 0.0004 0.0004 0.0005 0.0005 0.0009 0.0007 0.0002 0.0008 0.0005 0.0004 0.0009
Bud Periphery 0.0007 0.0001 0.0002 0.0002 0.0001 0.0005 0.001 0.0002 0.0014 0.0006 0.0019 0.0005 0.0014 0 0.0025 0.001 0.0002 0.0005
Bud Site 0.0002 0.0003 0.0001 0.0002 0.0001 0.0001 0.0008 0.0002 0.0011 0.0001 0.0006 0.0001 0.0009 0.0001 0.0009 0.0004 0.0004 0.0004
Cell Periphery 0.0201 0.0095 0.0143 0.0037 0.0076 0.004 0.0131 0.0117 0.0111 0.005 0.0111 0.0087 0.0141 0.0034 0.0091 0.0029 0.0015 0.0011
Cytoplasm 0.8029 0.9359 0.859 0.828 0.879 0.8153 0.7922 0.9467 0.8772 0.8683 0.7849 0.8803 0.796 0.9734 0.8746 0.8794 0.7946 0.7048
Cytoplasmic Foci 0.0008 0.0006 0.0005 0.0036 0.0011 0.002 0.0144 0.0003 0.011 0.007 0.0206 0.0014 0.0051 0.0002 0.0097 0.0017 0.0485 0.0107
Eisosomes 0 0.0002 0 0.0001 0 0 0.0005 0 0.0004 0 0.0001 0 0.0003 0 0.0003 0.0001 0.0004 0.0005
Endoplasmic Reticulum 0.0268 0.0078 0.0111 0.0032 0.002 0.0228 0.0326 0.0074 0.0098 0.0139 0.005 0.0434 0.0325 0.0044 0.0034 0.003 0.0021 0.0123
Endosome 0.0019 0.0007 0.0015 0.0025 0.0016 0.006 0.0081 0.0003 0.0008 0.0015 0.0226 0.0113 0.0029 0.0002 0.003 0.001 0.0018 0.0047
Golgi 0.0003 0.0002 0.0001 0.0001 0.0001 0.0007 0.0011 0 0.0001 0.0001 0.0035 0.001 0.0003 0 0.0013 0.0001 0.0005 0.0008
Lipid Particles 0.001 0.0008 0.0007 0.0017 0.0007 0.0007 0.0048 0.0005 0.0019 0.0011 0.0035 0.0011 0.0012 0.0001 0.0027 0.0007 0.0115 0.0021
Mitochondria 0.0013 0.0006 0.0004 0.0024 0.0014 0.0005 0.0094 0.0002 0.0046 0.0009 0.0196 0.0027 0.0083 0.0001 0.0048 0.0064 0.0046 0.0347
None 0.0012 0.0004 0.0003 0.0016 0.0005 0.0011 0.0077 0.0002 0.0086 0.0085 0.0106 0.0015 0.0033 0.0002 0.0056 0.0041 0.0231 0.0117
Nuclear Periphery 0.0371 0.0066 0.0124 0.0125 0.0091 0.0346 0.021 0.0036 0.0053 0.0109 0.0137 0.0071 0.0391 0.0026 0.0088 0.0203 0.0096 0.0048
Nucleolus 0.0043 0.0018 0.0043 0.0218 0.0234 0.0072 0.0125 0.0002 0.0059 0.0017 0.0027 0.0013 0.0059 0.0001 0.0044 0.0029 0.0187 0.1468
Nucleus 0.0095 0.0025 0.005 0.0101 0.0063 0.0141 0.0082 0.0022 0.004 0.0058 0.0068 0.0034 0.0186 0.002 0.0083 0.0164 0.0084 0.0417
Peroxisomes 0 0 0 0.0002 0.0001 0 0.0005 0 0.0003 0.0001 0.0005 0.0001 0.0003 0 0.0003 0.0003 0.0007 0.0006
Punctate Nuclear 0.0001 0.0001 0 0.0022 0.0001 0.0002 0.001 0 0.0015 0.0004 0.0008 0 0.0004 0 0.0008 0.0002 0.0045 0.0029
Vacuole 0.0772 0.026 0.079 0.0871 0.0529 0.0688 0.0568 0.0235 0.0463 0.0609 0.0694 0.0298 0.0532 0.012 0.043 0.0452 0.0512 0.0062
Vacuole Periphery 0.0132 0.005 0.0098 0.0177 0.0126 0.0191 0.0121 0.0015 0.0059 0.0116 0.0188 0.0045 0.0126 0.0008 0.0086 0.0115 0.0163 0.0094

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3505.0154 3668.4042 4179.0646 3539.3398 3986.4178 3478.9159 3274.5041 3025.021 3337.831 3054.6321
Translational Efficiency 1.3873 1.3589 1.3411 1.4852 1.4986 1.4004 1.3943 1.7216 1.4022 1.6203

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘