Standard name
Description Thymidylate synthase; required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S; human homolog TYMSOS can complement yeast cdc21 temperature-sensitive mutant at restrictive temperature

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.96 0.96 0.99 0.95 0.9 0.88 0.88 0.87 0.83 0.82 0.8 0.99 0.98 0.99 0.77 0.75 0.77 0.36 0.53 0.27 0.34 0.45 0.41
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0.05 0.16 0.21 0.17 0.23 0.29 0.31 0.22 0 0 0 0 0 0 0 0 0 0.05 0.05 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0 0.09 0.06 0 0.05 0.06 0.06 0.06 0.12 0 0 0 0.26 0.3 0.26 0.57 0.39 0.58 0.56 0.42 0.4
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 1 0 0 0 1 0 0 0 1 0 0 0 1 3 0 2 3
Bud 0 2 1 2 3 13 10 13 19 23 18 19 0 0 0 1 0 0 1 2 8 0 1 6
Bud Neck 0 0 0 0 0 0 1 4 1 0 1 1 0 1 0 0 0 0 0 4 4 0 1 2
Bud Site 0 0 0 0 0 0 0 0 2 2 0 0 0 0 0 0 0 0
Cell Periphery 1 0 0 1 0 1 3 0 1 0 1 0 2 4 1 1 0 0 0 0 0 0 0 2
Cytoplasm 203 341 518 487 672 821 1078 964 844 877 702 877 149 165 195 217 169 134 80 184 148 40 116 111
Endoplasmic Reticulum 3 1 1 0 1 0 1 1 1 2 1 0 1 3 1 19 10 5 1 0 9 1 3 4
Endosome 0 0 0 2 4 3 3 3 3 2 5 15 0 0 0 3 3 0 2 4 12 0 2 5
Golgi 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 0 1 0
Mitochondria 6 16 22 9 35 149 252 186 222 305 268 240 0 1 0 6 5 2 6 5 17 5 14 16
Nucleus 1 0 0 1 1 6 9 14 3 1 1 5 1 1 0 14 10 2 0 0 0 0 0 0
Nuclear Periphery 0 0 2 0 5 5 13 8 1 2 0 0 0 0 0 1 0 0 0 0 1 0 0 0
Nucleolus 0 0 2 0 0 2 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
SpindlePole 0 0 0 0 1 0 2 3 3 5 4 4 0 0 0 0 0 2 2 3 2 0 1 4
Vac/Vac Membrane 16 7 24 3 64 54 38 50 57 66 55 132 0 0 0 74 68 46 125 138 315 66 107 108
Unique Cell Count 215 354 541 493 711 913 1219 1095 974 1052 858 1100 150 168 196 281 224 175 222 352 540 119 257 271
Labelled Cell Count 231 368 571 505 786 1054 1411 1248 1160 1285 1056 1294 153 175 197 337 266 191 222 352 540 119 257 271


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.5 3.6 3.6 3.9 3.2 3.2 3.6 3.2 3.2 3.1 3.3 4.4 4.4 4.8 5.1 5.1 5.1 4.4 4.7 4.9
Std Deviation (1e-4) 0.5 0.5 1.0 1.4 1.4 1.3 1.0 1.3 1.2 0.8 0.9 0.7 0.6 0.7 1.0 0.8 1.0 1.0 0.9 1.1 1.4
Intensity Change (Log2) 0.01 0.12 -0.19 -0.17 0.02 -0.19 -0.16 -0.21 -0.13 0.29 0.3 0.41 0.52 0.51 0.52 0.28 0.38 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 9.2 10.0 8.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available