Standard name
Description FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of thenuclear pore complex (NPC) permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0.19 0.07 0.12 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.18 0.1 0.13 0.12 0.36 0.37 0.41 0.53 0.55 0.6 0.42 0 0 0 0.1 0.11 0.07 0 0 0 0.07 0.13 0.14
Nucleus 0.05 0 0 0 0 0 0 0 0 0 0.07 0 0.05 0.05 0 0.1 0.09 0.07 0 0 0 0 0 0
Nuclear Periphery 0.74 0.81 0.79 0.85 0.78 0.64 0.68 0.56 0.57 0.53 0.47 0.59 0.77 0.76 0.66 0.68 0.62 0.68 0.87 0.87 0.89 0.82 0.64 0.66
Nucleolus 0.1 0.12 0.19 0.25 0.21 0.27 0.28 0.33 0.29 0.29 0.32 0.35 0.06 0.06 0 0.11 0.11 0.13 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.38 0.21 0.16 0.09 0.17 0.09 0.07 0.06 0.07 0.05 0.05 0.06 0.25 0.26 0.3 0.3 0.27 0.29 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 0 1 0 1 0
Bud 0 0 0 0 0 1 0 2 2 1 4 2 0 0 0 2 1 1 0 1 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 2 1 4 1 10 0 4 4 1 0 1 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0
Cytoplasm 8 2 17 9 13 11 14 18 6 10 6 8 3 3 33 34 38 29 0 0 0 0 1 1
Endoplasmic Reticulum 11 0 0 0 0 0 0 2 0 0 1 0 0 0 0 16 15 5 0 0 0 0 3 3
Endosome 2 0 8 4 12 10 7 27 4 1 2 15 1 2 4 16 15 10 5 0 6 6 18 19
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 3 1 5 7
Mitochondria 15 21 25 30 29 139 162 261 201 245 239 208 1 1 2 52 37 20 6 2 6 16 38 37
Nucleus 14 5 4 4 3 9 12 19 5 14 26 18 6 6 4 49 31 20 3 2 1 2 5 0
Nuclear Periphery 192 92 206 200 189 243 298 353 215 234 190 296 87 87 117 338 206 196 252 104 237 200 188 176
Nucleolus 26 14 50 59 52 102 123 210 111 130 126 173 7 7 8 57 36 37 3 0 2 4 4 2
Peroxisomes 0 0 0 0 0 2 3 1 1 1 1 1 0 0 1 1 0 0 0 0 0 2 6 6
SpindlePole 0 0 0 0 0 0 3 5 2 3 2 5 4 4 4 4 4 2 1 0 0 2 2 1
Vac/Vac Membrane 99 24 43 20 41 33 31 39 25 21 20 29 28 30 53 151 88 83 11 4 5 6 13 7
Unique Cell Count 261 114 262 235 243 381 437 635 377 444 400 498 113 114 178 498 330 290 290 120 268 245 296 268
Labelled Cell Count 367 158 353 326 339 551 655 938 577 661 628 755 141 144 227 720 474 405 290 120 268 245 296 268


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.2 10.4 10.3 10.1 9.2 7.7 7.3 7.2 7.1 6.4 6.5 6.7 9.6 9.6 9.6 7.9 8.4 11.3 10.9 11.1 11.4
Std Deviation (1e-4) 3.9 2.5 2.3 2.0 2.1 1.7 2.1 2.0 1.9 1.9 1.9 1.7 2.0 2.0 2.8 1.9 2.5 3.2 2.2 2.2 2.0
Intensity Change (Log2) -0.03 -0.17 -0.41 -0.51 -0.51 -0.53 -0.68 -0.66 -0.61 -0.1 -0.1 -0.1 -0.39 -0.3 0.13 0.07 0.11 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 -0.5 -2.2 -2.0 -2.6 -3.3 -2.8 -3.4 -3.6 -1.5 -1.5 3.9 0.2 2.1 1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.9 3.5 0
Endosome -1.0 1.1 -0.3 -1.3 0.8 0 0 0 0 0 0 0 0.1 0.9 0.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.1 0.9 7.7 8.0 9.2 11.4 12.1 12.9 9.2 -3.0 -3.0 -3.6 0.4 0.7 -1.1
Nucleus 0 0 0.7 1.0 1.3 0 1.3 3.0 1.6 0 0 0 4.3 4.0 3.1
Nuclear Periphery 1.9 -0.2 -4.0 -3.0 -6.5 -5.7 -6.9 -8.0 -5.3 -0.4 -0.5 -3.0 -3.1 -4.3 -2.9
Nucleolus 1.6 0.6 2.3 2.7 4.2 3.0 3.0 3.5 4.5 -3.2 -3.2 -4.4 -2.9 -2.8 -2.0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.6 0.1 -3.0 -3.9 -4.9 -3.9 -5.2 -4.9 -4.7 1.9 2.2 3.3 4.2 3.0 3.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nup1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available