Standard name
Human Ortholog
Description Ubiquitin-specific protease; removes ubiquitin from ubiquitinated proteins; controls K63 homeostasis during oxidative stress; deubiquitinates Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 0.94 0.91 0.86 0.84 0.79 0.72 0.68 0.64 0.65 0.95 0.9 0.87 0.8 0.71 0.73 0.93 0.94 0.89 0.9 0.91 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.1 0.1 0.31 0.26 0.26 0.47 0.5 0.62 0.56 0 0 0.06 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0.05 0 0.06 0 0.05 0.05 0.06 0 0.11 0.18 0.21 0.35 0.28 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 1
Bud 0 1 3 1 0 7 5 2 4 6 3 6 0 0 2 0 0 1 0 0 1 0 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1
Bud Site 0 0 0 0 0 0 0 3 0 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 1 0 2 3 2 4 1 6 6 0 0 0 2 2 5 0 0 0 0 0 0
Cytoplasm 237 286 264 200 273 342 461 402 284 293 240 289 143 220 232 197 129 122 227 279 268 151 193 175
Endoplasmic Reticulum 5 0 0 0 1 0 0 0 1 2 2 2 1 0 0 12 6 7 1 1 1 2 2 2
Endosome 0 0 0 1 0 0 0 2 0 0 0 0 1 1 0 6 3 1 1 0 3 1 3 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 2 2 0 0 0 0 0
Mitochondria 0 2 5 22 29 124 143 134 184 215 232 251 0 0 16 7 1 6 1 3 7 1 0 1
Nucleus 1 1 0 2 1 5 6 13 5 6 6 6 2 2 3 6 5 3 2 4 0 0 0 1
Nuclear Periphery 0 1 0 0 0 1 2 1 3 1 1 7 0 0 0 1 0 0 1 1 0 1 0 0
Nucleolus 0 1 0 0 1 0 0 3 1 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Vac/Vac Membrane 1 3 2 5 21 18 23 28 11 21 19 27 6 28 47 52 64 47 2 2 11 4 2 4
Unique Cell Count 242 289 269 213 299 398 549 506 393 432 377 445 150 245 268 246 181 167 244 298 301 169 212 198
Labelled Cell Count 245 295 274 232 326 499 643 591 497 547 510 595 153 251 300 288 210 196 244 298 301 169 212 198


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 6.0 4.8 4.8 5.2 4.5 4.4 4.6 3.9 3.9 3.7 3.6 6.4 6.6 5.9 9.4 10.1 10.1 6.0 6.7 7.1
Std Deviation (1e-4) 0.8 0.9 0.6 1.1 1.3 1.1 1.2 1.2 1.1 1.0 0.8 1.3 0.9 1.2 1.2 1.6 2.1 2.1 1.6 1.3 1.2
Intensity Change (Log2) – – – -0.01 0.11 -0.1 -0.12 -0.06 -0.29 -0.31 -0.36 -0.4 0.42 0.46 0.31 0.97 1.08 1.08 0.32 0.48 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.4 -3.6 -5.4 -6.0 -7.1 -8.7 -9.7 -10.4 -10.3 -1.7 -4.0 -5.0 -6.7 -8.4 -8.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.7 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.9 0 0 0 0 0 0 0 0 0 2.5 0.7 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.8 2.8 2.7 3.3 1.9 3.0 3.0 3.5 0 5.2 6.8 7.5 10.2 8.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8716 5.4244 5.003 4.7165 4.2216 5.0947 3.3298 5.0314 4.3717 4.2409 3.5329 4.4969 4.5886 5.5872 5.216 5.0909 5.472 5.1368
Actin 0.0016 0.0011 0.0018 0.0013 0.0003 0.0027 0.0183 0.001 0.0193 0.0569 0.002 0.0041 0.0172 0.001 0.0178 0.0465 0.019 0.0036
Bud 0.0001 0.0007 0.0008 0.0004 0.0001 0.0002 0.0003 0.0003 0.0008 0.0009 0.0006 0.0002 0.002 0.0016 0.0006 0.0021 0.0001 0.0016
Bud Neck 0.0004 0.0004 0.0008 0.0011 0.0011 0.0027 0.0016 0.0003 0.0004 0.0033 0.0008 0.0012 0.0004 0.0002 0.0109 0.0008 0.0006 0.0013
Bud Periphery 0.0001 0.0008 0.0004 0.0006 0 0.0001 0.0002 0.0005 0.0005 0.0009 0.001 0.0002 0.0016 0.0009 0.0005 0.0032 0.0001 0.0038
Bud Site 0.0003 0.0024 0.0026 0.0003 0.0002 0.0002 0.0044 0.0052 0.001 0.0053 0.0008 0.0004 0.0186 0.0014 0.003 0.0032 0.0001 0.0003
Cell Periphery 0.0001 0.0004 0.0002 0.0002 0 0.0001 0.0002 0.0003 0.0001 0.0009 0.0001 0 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001
Cytoplasm 0.9272 0.9371 0.9184 0.9062 0.9022 0.9243 0.6912 0.9058 0.8299 0.6387 0.7957 0.8479 0.7593 0.8493 0.7821 0.8642 0.7669 0.8571
Cytoplasmic Foci 0.0041 0.0056 0.0045 0.0035 0.0036 0.0093 0.0561 0.0058 0.0161 0.0341 0.0496 0.0109 0.013 0.0055 0.0182 0.0069 0.0063 0.0092
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0.0005 0 0.0001 0.0001 0 0.0002 0.0002 0.0002 0
Endoplasmic Reticulum 0.004 0.0067 0.0053 0.005 0.0056 0.0067 0.006 0.0014 0.0026 0.002 0.0047 0.0022 0.0052 0.0024 0.0041 0.0024 0.0101 0.0032
Endosome 0.0021 0.0024 0.0035 0.0026 0.004 0.0054 0.0195 0.0039 0.0106 0.0276 0.0348 0.0109 0.0137 0.0009 0.0108 0.0099 0.0154 0.0048
Golgi 0.0002 0.0004 0.0008 0.0002 0.0001 0.0021 0.0069 0.001 0.0068 0.0302 0.0088 0.005 0.007 0.0002 0.0054 0.0079 0.0032 0.0043
Lipid Particles 0.0001 0.0003 0.0002 0.0001 0 0.0001 0.0119 0.0005 0.0084 0.0049 0.0104 0.003 0.0163 0.0001 0.0042 0.0009 0.0011 0.0012
Mitochondria 0.0002 0.0033 0.0109 0.0004 0.0004 0.0008 0.0056 0.0012 0.0128 0.0068 0.0142 0.0031 0.0028 0.0002 0.0017 0.0119 0.0008 0.0069
None 0.0399 0.0165 0.0156 0.0094 0.0099 0.0137 0.0942 0.0661 0.0672 0.1556 0.0407 0.0856 0.0672 0.1307 0.1137 0.0224 0.1371 0.0527
Nuclear Periphery 0.0019 0.0023 0.0028 0.004 0.0066 0.0031 0.0056 0.0007 0.0025 0.0016 0.0035 0.0054 0.0218 0.0006 0.0021 0.0019 0.0266 0.003
Nucleolus 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0007 0.0001 0.0001 0.0001 0.0002 0.0002 0.0003 0 0.0002 0.0001 0.0002 0.0001
Nucleus 0.014 0.0113 0.028 0.0617 0.0609 0.0223 0.0107 0.0037 0.0103 0.0095 0.0115 0.0146 0.043 0.0033 0.0102 0.0117 0.0075 0.0323
Peroxisomes 0.0001 0.0002 0.0004 0.0001 0.0001 0.0007 0.0355 0.0004 0.006 0.0167 0.0096 0.0017 0.0043 0.0004 0.0074 0.0007 0.0002 0.001
Punctate Nuclear 0.0004 0.0062 0.0011 0.0016 0.0009 0.0022 0.0288 0.0005 0.0036 0.0024 0.007 0.0023 0.0028 0.0007 0.0053 0.001 0.0026 0.0126
Vacuole 0.0028 0.0017 0.0015 0.0012 0.0032 0.003 0.0015 0.0012 0.0008 0.0008 0.0026 0.0007 0.001 0.0003 0.0009 0.0013 0.0006 0.0006
Vacuole Periphery 0.0003 0.0002 0.0003 0.0002 0.0004 0.0003 0.0005 0.0001 0.0003 0.0004 0.0013 0.0004 0.0022 0 0.0003 0.0005 0.0011 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.3821 26.8613 29.3233 44.1016 29.5937 30.8979 33.0352 36.6615 39.6541 40.2297
Translational Efficiency 1.1814 1.063 1.0968 0.8797 1.0916 1.1633 1.0001 0.7808 0.9068 0.8816

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘