Standard name
Description Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.17 0.12 0.14 0.25 0.32 0.28 0.34 0.22 0.28 0.1 0.1 0.1 0.06 0.15 0 0.07 0 0 0 0
Endoplasmic Reticulum 0 0 0 0.09 0 0 0.08 0 0 0 0 0 0.07 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0.11 0.1 0.11 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Nucleus 0.39 0.53 0.53 0 0.05 0.11 0.1 0.15 0.15 0.26 0.19 0.21 0.64 0.67 0.5 0.91 0.63 0.53 0.5 0.67 0.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.22 0.06 0.06 0 0 0 0.08 0.05 0.15 0 0.19 0.1 0.05 0 0.05 0 0.05 0.13 0.17 0.08 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.44 0.31 0.35 0.69 0.62 0.46 0.44 0.49 0.5 0.43 0.52 0.62 0.19 0.21 0.25 0.05 0.11 0.19 0.19 0.13 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 6 2 5 16 36 11 14 9 16 2 5 4 3 3 0 4 1 3 4 4
Endoplasmic Reticulum 0 0 0 3 0 3 3 0 0 0 0 0 3 3 1 0 2 0 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 3
Golgi 0 1 0 4 6 12 1 1 0 1 0 1 1 2 1 0 0 0 1 0 2
Mitochondria 0 0 0 0 0 1 0 0 1 0 0 0 3 0 0 0 0 0 1 0 1
Nucleus 7 19 9 1 3 12 4 6 6 15 4 10 27 35 10 11 43 46 39 91 78
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 1 2 4
Nucleolus 4 2 1 0 2 4 3 2 6 2 4 5 2 1 1 0 3 11 13 11 16
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 1
Vac/Vac Membrane 8 11 6 24 39 51 17 20 20 25 11 30 8 11 5 0 7 16 14 17 33
Unique Cell Count 18 36 17 35 63 112 39 41 40 58 21 48 42 52 20 13 69 87 79 137 158
Labelled Cell Count 20 39 18 37 66 119 39 43 42 59 21 51 48 55 22 13 69 87 79 137 158


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 52.4 64.4 73.4 66.2 68.4 61.9 56.3 57.4 46.3 55.6 65.4 60.7 35.6 50.4 45.3 57.1 62.0 64.4
Std Deviation (1e-4) 18.9 23.4 23.9 24.2 26.6 25.8 24.8 22.2 27.2 22.5 21.2 22.6 11.7 13.5 14.9 23.1 21.8 24.5
Intensity Change (Log2) 0.19 0.04 0.09 -0.06 -0.19 -0.17 -0.48 -0.21 0.02 -0.09 -0.86 -0.35 -0.51 -0.17 -0.06 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -0.3 1.0 1.8 1.2 1.7 0.6 1.2 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 -4.7 -5.5 -5.4 -4.0 -3.6 -3.5 -2.6 -2.5 -3.0 1.0 1.4 -0.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.3 3.2 3.0 1.6 1.2 1.6 1.7 1.2 1.6 2.9 -1.2 -1.0 -0.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pup1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available