Standard name
Description Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.06 0.17 0.12 – 0.14 0.25 0.32 0.28 0.34 0.22 0.28 – 0.1 0.1 0.1 0.06 0.15 0 0.07 0 0 0 0
Endoplasmic Reticulum – 0 0 0 – 0.09 0 0 0.08 0 0 0 – 0 0 0.07 0.06 0.05 0 0 0 0 0 0
Endosome – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0
Golgi – 0 0 0 – 0.11 0.1 0.11 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0.07 0 0 0 0 0 0 0 0
Nucleus – 0.39 0.53 0.53 – 0 0.05 0.11 0.1 0.15 0.15 0.26 – 0.19 0.21 0.64 0.67 0.5 0.91 0.63 0.53 0.5 0.67 0.5
Nuclear Periphery – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0.22 0.06 0.06 – 0 0 0 0.08 0.05 0.15 0 – 0.19 0.1 0.05 0 0.05 0 0.05 0.13 0.17 0.08 0.1
Peroxisomes – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.44 0.31 0.35 – 0.69 0.62 0.46 0.44 0.49 0.5 0.43 – 0.52 0.62 0.19 0.21 0.25 0.05 0.11 0.19 0.19 0.13 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0
Bud – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 2 0 1 2 3
Bud Neck – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 1 6 2 – 5 16 36 11 14 9 16 – 2 5 4 3 3 0 4 1 3 4 4
Endoplasmic Reticulum – 0 0 0 – 3 0 3 3 0 0 0 – 0 0 3 3 1 0 2 0 0 0 1
Endosome – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 3
Golgi – 0 1 0 – 4 6 12 1 1 0 1 – 0 1 1 2 1 0 0 0 1 0 2
Mitochondria – 0 0 0 – 0 0 1 0 0 1 0 – 0 0 3 0 0 0 0 0 1 0 1
Nucleus – 7 19 9 – 1 3 12 4 6 6 15 – 4 10 27 35 10 11 43 46 39 91 78
Nuclear Periphery – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 3 1 2 4
Nucleolus – 4 2 1 – 0 2 4 3 2 6 2 – 4 5 2 1 1 0 3 11 13 11 16
Peroxisomes – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 0 1 1
Vac/Vac Membrane – 8 11 6 – 24 39 51 17 20 20 25 – 11 30 8 11 5 0 7 16 14 17 33
Unique Cell Count 18 36 17 35 63 112 39 41 40 58 21 48 42 52 20 13 69 87 79 137 158
Labelled Cell Count 20 39 18 37 66 119 39 43 42 59 21 51 48 55 22 13 69 87 79 137 158


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 52.4 64.4 73.4 – 66.2 68.4 61.9 56.3 57.4 46.3 55.6 – 65.4 60.7 35.6 50.4 45.3 57.1 62.0 64.4
Std Deviation (1e-4) – 18.9 23.4 23.9 – 24.2 26.6 25.8 24.8 22.2 27.2 22.5 – 21.2 22.6 11.7 13.5 14.9 23.1 21.8 24.5
Intensity Change (Log2) – – – 0.19 – 0.04 0.09 -0.06 -0.19 -0.17 -0.48 -0.21 – 0.02 -0.09 -0.86 -0.35 -0.51 -0.17 -0.06 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm 0 – -0.3 1.0 1.8 1.2 1.7 0.6 1.2 – 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleus 0 – -4.7 -5.5 -5.4 -4.0 -3.6 -3.5 -2.6 – -2.5 -3.0 1.0 1.4 -0.2
Nuclear Periphery 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0.3 – 3.2 3.0 1.6 1.2 1.6 1.7 1.2 – 1.6 2.9 -1.2 -1.0 -0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 30.0985 44.7551 41.0678 32.9252 36.4703 36.7402 41.9896 50.3414 43.2177 39.0576 38.5149 39.5074 32.4609 46.2701 42.4216 40.3524 37.044 39.9833
Actin 0.0322 0.0013 0.0027 0.001 0.0106 0.0049 0.0003 0.0003 0.0022 0.0238 0.0001 0.0008 0.008 0.0001 0 0.0003 0.003 0.0066
Bud 0.0023 0.0004 0.0002 0.0001 0.0016 0.0005 0.0001 0.0001 0.0005 0.0031 0.0001 0.0003 0.0006 0 0 0 0.0001 0.0005
Bud Neck 0.0024 0.0001 0.0001 0.0001 0.0007 0.0004 0 0 0.0001 0.0007 0.0003 0.0005 0.0002 0 0 0 0.0001 0.0004
Bud Periphery 0.0018 0.0005 0.0002 0.0002 0.004 0.0012 0 0.0001 0.0003 0.0025 0.0002 0.0004 0.0008 0 0 0 0.0001 0.0003
Bud Site 0.0116 0.0005 0.0002 0 0.0024 0.0004 0.0001 0.0003 0.0002 0.0138 0 0.0001 0.0003 0 0 0 0.0001 0.0004
Cell Periphery 0.0009 0.0002 0 0.0002 0.0005 0.0005 0 0.0002 0.0001 0.0004 0.0001 0.0001 0.0003 0 0 0 0 0.0001
Cytoplasm 0.0074 0.0012 0.0035 0.0015 0.0007 0.0009 0.0022 0.0103 0.0041 0.0007 0.0013 0.001 0.0425 0.0063 0.0003 0.0019 0.0007 0.0088
Cytoplasmic Foci 0.0035 0.0009 0.0001 0 0.0002 0.0006 0 0.0003 0.0001 0.0011 0 0 0.0003 0 0 0 0.0003 0.005
Eisosomes 0.0003 0.0001 0.0001 0 0.0003 0 0 0 0 0.001 0 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0033 0.0004 0.0034 0.0013 0.0021 0.0037 0.0001 0 0.0001 0.0002 0 0.0001 0.0023 0 0 0.0001 0.0004 0.0012
Endosome 0.017 0.0061 0.0009 0.0004 0.0022 0.0136 0 0 0.0002 0.0005 0.0001 0.0001 0.0025 0 0 0.0002 0.0014 0.0066
Golgi 0.0024 0.0012 0.0001 0 0.0006 0.0021 0 0 0.0001 0.0009 0 0 0.0018 0 0 0 0.0003 0.0019
Lipid Particles 0.0022 0.0011 0.0001 0 0.0002 0.002 0 0.0004 0 0.0004 0 0 0.0014 0 0 0 0.0003 0.0019
Mitochondria 0.0107 0.0064 0.006 0.0336 0.0753 0.0245 0.0012 0.0032 0.0025 0.0177 0.0016 0.0015 0.0136 0.0009 0.0005 0.0012 0.0017 0.0023
None 0.0018 0.0003 0.0004 0.0002 0.0004 0.0004 0.0001 0.0002 0.0001 0.0009 0 0.0002 0.006 0 0 0.0001 0.0002 0.0006
Nuclear Periphery 0.1013 0.0119 0.0348 0.1302 0.0208 0.0505 0.0158 0.0166 0.0226 0.0066 0.0064 0.0158 0.0815 0.0098 0.0042 0.0251 0.0724 0.0262
Nucleolus 0.0303 0.0132 0.0102 0.0042 0.0257 0.0315 0.0094 0.035 0.0066 0.0303 0.0289 0.0149 0.0104 0.0117 0.0122 0.0023 0.0155 0.017
Nucleus 0.7192 0.9506 0.9339 0.8049 0.8459 0.8287 0.9697 0.9308 0.949 0.8827 0.9566 0.9613 0.8179 0.9702 0.9778 0.9666 0.8957 0.9055
Peroxisomes 0.0006 0.0002 0.0001 0 0.0002 0.0002 0 0.0001 0.0001 0.0008 0 0 0.0002 0 0 0 0.0001 0.0006
Punctate Nuclear 0.0179 0.0002 0.0013 0.0001 0.0004 0.0009 0.0006 0.0006 0.0091 0.0109 0.0002 0.0012 0.0012 0.0001 0.0047 0.0011 0.0059 0.0115
Vacuole 0.0202 0.0016 0.0004 0.002 0.0021 0.0108 0.0001 0.0004 0.0003 0.0004 0.0017 0.0005 0.0024 0.0001 0 0.0002 0.0003 0.0008
Vacuole Periphery 0.0108 0.0018 0.0013 0.0198 0.0033 0.0217 0.0003 0.0011 0.0016 0.0006 0.0024 0.0012 0.0056 0.0006 0.0002 0.0006 0.0014 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 152.3147 110.434 124.7826 159.6693 121.0378 113.6467 147.1878 155.8639 160.2607 131.9533
Translational Efficiency 1.9229 2.4173 2.1409 1.7212 1.9051 2.6361 1.834 1.9988 1.7403 1.6401

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘