Standard name | |
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Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 0 | 1 | 0 | 0 | 6 | 3 | 1 | 5 | 2 | 4 | 0 | 1 | 0 | 4 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 144 | 158 | 41 | 96 | 118 | 81 | 145 | 97 | 98 | 120 | 71 | 99 | 141 | 260 | 303 | 134 | 104 | 129 | 151 | 179 | 64 | 122 | 216 | 199 |
Endoplasmic Reticulum | 0 | 3 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 4 | 0 | 2 | 0 | 0 | 0 | 7 | 3 | 5 | 0 | 0 | 0 | 1 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3 | 8 | 1 | 3 | 2 | 8 | 4 | 10 | 23 | 32 | 43 | 33 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 0 | 2 | 0 | 1 |
Nucleus | 4 | 0 | 1 | 3 | 1 | 2 | 1 | 5 | 4 | 4 | 0 | 3 | 1 | 2 | 1 | 1 | 1 | 2 | 1 | 0 | 0 | 2 | 2 | 4 |
Nuclear Periphery | 4 | 0 | 0 | 1 | 0 | 4 | 1 | 0 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 |
Vac/Vac Membrane | 4 | 0 | 0 | 8 | 6 | 3 | 22 | 19 | 20 | 23 | 17 | 25 | 1 | 3 | 3 | 1 | 3 | 6 | 14 | 1 | 0 | 1 | 0 | 2 |
Unique Cell Count | 153 | 167 | 43 | 107 | 126 | 92 | 171 | 124 | 134 | 171 | 118 | 151 | 144 | 264 | 304 | 135 | 108 | 135 | 177 | 189 | 73 | 136 | 234 | 225 |
Labelled Cell Count | 161 | 171 | 43 | 112 | 128 | 101 | 182 | 136 | 149 | 190 | 134 | 171 | 144 | 268 | 308 | 147 | 112 | 147 | 177 | 189 | 73 | 136 | 234 | 225 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 4.5 | 7.3 | 7.4 | 9.7 | 7.9 | 9.4 | 8.9 | 8.4 | 8.9 | 8.3 | 8.9 | 8.3 | 9.0 | 9.8 | 9.7 | 12.3 | 11.5 | 9.8 | 11.5 | 13.3 |
Std Deviation (1e-4) | 1.8 | 1.0 | 1.9 | 1.8 | 2.4 | 2.4 | 2.3 | 2.2 | 2.6 | 2.6 | 2.4 | 3.3 | 2.0 | 2.0 | 2.4 | 3.1 | 4.9 | 3.7 | 3.0 | 3.5 | 3.9 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.41 | 0.12 | 0.37 | 0.29 | 0.21 | 0.28 | 0.2 | 0.29 | 0.19 | 0.31 | 0.42 | 0.42 | 0.76 | 0.65 | 0.43 | 0.66 | 0.87 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |