Standard name
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.95 0.95 0.9 0.94 0.88 0.85 0.78 0.73 0.7 0.6 0.66 0.98 0.98 1.0 0.99 0.96 0.96 0.86 0.95 0.88 0.9 0.93 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.09 0 0.08 0.17 0.19 0.36 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.07 0.05 0 0.13 0.15 0.15 0.13 0.14 0.17 0 0 0 0 0 0 0.08 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0 0 0 0 1 1
Bud 0 2 0 0 0 1 1 0 1 0 0 1 0 0 0 0 0 0 0 0 1 1 5 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 0 0 1 0 0 6 3 1 5 2 4 0 1 0 4 0 2 0 0 0 0 0 0
Cytoplasm 144 158 41 96 118 81 145 97 98 120 71 99 141 260 303 134 104 129 151 179 64 122 216 199
Endoplasmic Reticulum 0 3 0 0 1 1 2 0 1 4 0 2 0 0 0 7 3 5 0 0 0 1 1 1
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 0 1 1 1 0 0 0 0 0
Mitochondria 3 8 1 3 2 8 4 10 23 32 43 33 1 0 0 0 0 1 2 3 0 2 0 1
Nucleus 4 0 1 3 1 2 1 5 4 4 0 3 1 2 1 1 1 2 1 0 0 2 2 4
Nuclear Periphery 4 0 0 1 0 4 1 0 1 2 1 2 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 0
Vac/Vac Membrane 4 0 0 8 6 3 22 19 20 23 17 25 1 3 3 1 3 6 14 1 0 1 0 2
Unique Cell Count 153 167 43 107 126 92 171 124 134 171 118 151 144 264 304 135 108 135 177 189 73 136 234 225
Labelled Cell Count 161 171 43 112 128 101 182 136 149 190 134 171 144 268 308 147 112 147 177 189 73 136 234 225


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 4.5 7.3 7.4 9.7 7.9 9.4 8.9 8.4 8.9 8.3 8.9 8.3 9.0 9.8 9.7 12.3 11.5 9.8 11.5 13.3
Std Deviation (1e-4) 1.8 1.0 1.9 1.8 2.4 2.4 2.3 2.2 2.6 2.6 2.4 3.3 2.0 2.0 2.4 3.1 4.9 3.7 3.0 3.5 3.9
Intensity Change (Log2) 0.03 0.41 0.12 0.37 0.29 0.21 0.28 0.2 0.29 0.19 0.31 0.42 0.42 0.76 0.65 0.43 0.66 0.87


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole