Standard name
Description Small-subunit processome protein involved in pre-18S rRNA maturation; part of a subunit of the 90S preribosomal particle capable of interacting directly with the 5' ETS of the 35S pre-rRNA; contains WD40 repeats
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0 0 0 0.05 0 0 0 0 0.06 – – 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0.09 0.08 0.06 0.06 0.07 0.06 0 0 – – 0 0 0 0 0 0 0.1 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.05 0.08 0.08 0.13 0.05 0.06 – – 0.09 0.1 0.09 0 0 0 0 0 0
Nucleus 0.15 0.18 0.16 0.05 0.09 0.09 0.08 0.08 0.05 0.1 0.09 0.12 0.06 – – 0.19 0.13 0.17 0.09 0.13 0.06 0.08 0.09 0.08
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0.05 0.06
Nucleolus 0.94 0.91 0.8 0.88 0.87 0.86 0.86 0.83 0.87 0.8 0.86 0.82 0.81 – – 0.82 0.85 0.84 0.84 0.79 0.82 0.37 0.52 0.44
Peroxisomes 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.14 0.12 0.19 0.24 0.27 0.28 0.29 0.26 0.18 0.23 0 – – 0 0 0 0 0 0.06 0.22 0.12 0.14
Vac/Vac Membrane 0 0 0.05 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 1 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 3 1 5 4 2 0 – – 1 0 0 1 3 0 0 0 0
Bud Neck 2 3 1 2 1 1 2 6 6 4 5 3 1 – – 0 1 3 0 0 0 0 0 0
Bud Site 1 1 0 1 0 6 3 1 5 9 4 10 0 – – 2 0 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 1 0 0 0 0 0 0
Cytoplasm 0 1 3 10 8 8 10 16 2 6 9 7 1 – – 11 3 7 0 0 1 0 0 1
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 0 0 0 0 – – 3 0 0 0 0 0 0 0 0
Endosome 0 2 0 2 11 20 20 19 14 19 16 14 0 – – 3 3 3 0 2 0 1 0 0
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 – – 1 0 0 1 0 0 0 0 0
Mitochondria 6 8 1 2 1 9 7 14 18 21 34 15 1 – – 23 20 20 2 2 0 0 0 0
Nucleus 30 58 12 7 18 20 22 24 11 27 25 38 1 – – 49 26 39 18 45 5 1 1 3
Nuclear Periphery 0 1 0 0 0 1 1 1 3 4 0 0 0 – – 0 2 0 0 0 0 0 1 2
Nucleolus 187 289 59 126 180 191 228 250 197 224 228 265 13 – – 209 170 192 170 268 75 4 12 18
Peroxisomes 0 1 0 1 3 12 2 3 5 2 5 3 0 – – 1 1 1 0 0 0 0 0 0
SpindlePole 6 14 10 17 39 54 72 84 66 73 49 73 0 – – 1 4 6 6 11 5 2 2 5
Vac/Vac Membrane 1 3 4 1 2 3 1 1 1 2 0 2 0 – – 4 5 6 2 2 0 0 0 0
Unique Cell Count 198 317 74 143 208 223 264 301 226 279 265 324 16 254 199 228 204 340 91 13 23 41
Labelled Cell Count 233 381 90 169 265 325 369 422 329 396 379 432 17 308 236 280 204 340 91 13 23 41


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.6 16.3 15.9 14.4 13.2 11.3 10.4 10.1 9.0 9.0 8.4 8.9 11.2 – – 26.3 27.1 24.1 – – –
Std Deviation (1e-4) 3.6 4.9 4.6 5.2 3.0 3.1 2.7 2.4 2.8 2.7 2.7 2.4 4.3 – – 11.5 11.2 12.9 – – –
Intensity Change (Log2) – – – -0.14 -0.26 -0.5 -0.61 -0.66 -0.83 -0.82 -0.92 -0.84 -0.51 – – 0.73 0.77 0.6 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Mitochondria 0 0 0 0 0 0 0 2.9 0 0 – – 2.2 2.4 2.2
Nucleus -2.8 -1.8 -1.7 -2.0 -2.2 -3.2 -1.6 -1.7 -1.1 0 – – 0.6 -0.7 0.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Nucleolus 1.7 1.4 1.2 1.4 0.7 1.6 0.1 1.3 0.4 0.1 – – 0.5 1.1 0.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
SpindlePole -0.3 1.0 1.9 2.4 2.6 2.7 2.3 1.0 1.7 0 – – 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.0119 13.718 11.3016 9.8537 11.0755 12.4415 10.273 13.5709 11.18 10.6115 9.0438 10.613 11.0844 12.0642 11.0048 10.9604 10.315 11.6755
Actin 0.0033 0 0.0014 0.0005 0 0.0001 0.0046 0 0.0001 0.0037 0 0.0005 0.0222 0 0.003 0.0193 0 0
Bud 0.0001 0 0.0004 0.0005 0.0001 0 0.0001 0 0.0001 0.0001 0 0 0.0002 0 0 0.0001 0 0
Bud Neck 0.0003 0.0001 0.0017 0.0002 0.0003 0.0001 0.0015 0.0001 0.0001 0.0031 0.0004 0.0001 0.0013 0.0001 0.0002 0.0001 0.0001 0.0001
Bud Periphery 0.0002 0 0.0011 0.0003 0.0001 0 0.0002 0 0.0001 0.0003 0 0.0001 0.0003 0 0 0.0001 0 0
Bud Site 0.0026 0 0.0004 0.001 0.0002 0 0.0009 0.0001 0.0002 0.0005 0.0001 0 0.0017 0 0.0002 0.0003 0.0002 0
Cell Periphery 0.0002 0 0.0001 0.0004 0.0003 0.0001 0.0002 0.0001 0.0001 0.0004 0.0001 0 0.0002 0.0001 0.0001 0.0002 0.0004 0.0001
Cytoplasm 0.0011 0.0001 0.0002 0.0004 0.0003 0.0004 0.0027 0 0.0001 0.001 0 0 0.0009 0 0.0008 0.0007 0.0001 0
Cytoplasmic Foci 0.0117 0 0.008 0.0273 0.0048 0.0032 0.0112 0.0001 0.0011 0.0087 0.0092 0.0003 0.0056 0 0.0044 0.0074 0.0059 0.0001
Eisosomes 0.0001 0 0 0.0005 0 0 0.0006 0 0 0.0008 0.0001 0 0.0002 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0002 0 0.0001 0.0001 0 0.0001 0.0009 0 0.0004 0.007 0 0 0.0007 0 0.0007 0.0006 0 0
Endosome 0.0021 0.0001 0.0014 0.0002 0.0009 0.0004 0.0059 0 0.0006 0.007 0.0001 0.0004 0.0037 0 0.0042 0.0022 0.0001 0
Golgi 0.0012 0 0.0021 0.0004 0.0002 0.0001 0.0017 0 0.0001 0.0019 0.0001 0.0002 0.0015 0 0.0008 0.001 0.0001 0
Lipid Particles 0.0078 0 0.0097 0.0137 0.0099 0.0035 0.0082 0.0001 0.006 0.0101 0.0074 0.0022 0.0058 0.0001 0.0035 0.008 0.0028 0.0003
Mitochondria 0.0031 0.0001 0.0009 0.0058 0.0027 0.0002 0.0042 0 0.0005 0.0077 0.0058 0.0005 0.0023 0.0001 0.0008 0.0052 0.0039 0.0001
None 0.0009 0 0.0001 0.0022 0.0002 0.0004 0.0081 0 0.0001 0.0057 0.0003 0.0003 0.0014 0 0.002 0.0016 0.0003 0
Nuclear Periphery 0.0017 0.0004 0.0001 0.0003 0.0007 0.0037 0.0021 0 0.0012 0.005 0 0.001 0.0014 0 0.0051 0.0013 0.0001 0
Nucleolus 0.8895 0.8741 0.8961 0.8473 0.8858 0.9246 0.8631 0.9106 0.9007 0.8339 0.9054 0.9242 0.865 0.9039 0.8819 0.8477 0.9222 0.9225
Nucleus 0.0681 0.1206 0.0718 0.0927 0.0807 0.0608 0.07 0.0869 0.084 0.0837 0.0624 0.0681 0.0681 0.0953 0.089 0.0976 0.058 0.0762
Peroxisomes 0.0009 0 0.0016 0.0017 0.0002 0.0001 0.0022 0 0.0002 0.0133 0.0007 0.0003 0.0086 0 0.0006 0.0013 0.0003 0
Punctate Nuclear 0.0031 0.0001 0.0017 0.0026 0.0055 0.0016 0.0102 0.0018 0.0027 0.0036 0.0072 0.0014 0.0083 0.0002 0.0019 0.0038 0.0035 0.0004
Vacuole 0.0011 0.0022 0.0009 0.0009 0.003 0.0002 0.001 0.0002 0.0014 0.0012 0.0003 0.0001 0.0006 0.0001 0.0003 0.0006 0.0008 0.0001
Vacuole Periphery 0.0007 0.0021 0.0002 0.0009 0.0041 0.0002 0.0006 0 0.0004 0.0013 0.0005 0.0002 0.0003 0 0.0004 0.0009 0.001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.0165 41.4444 41.4147 33.5991 44.8819 24.7125 27.3775 23.1918 24.1134 36.2491
Translational Efficiency 2.034 1.4531 1.4788 1.4169 1.1295 1.9171 1.7828 1.3422 1.319 1.2244

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘