Standard name
Description Small-subunit processome protein involved in pre-18S rRNA maturation; part of a subunit of the 90S preribosomal particle capable of interacting directly with the 5' ETS of the 35S pre-rRNA; contains WD40 repeats

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0 0 0 0.05 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0.09 0.08 0.06 0.06 0.07 0.06 0 0 0 0 0 0 0 0 0.1 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.05 0.08 0.08 0.13 0.05 0.06 0.09 0.1 0.09 0 0 0 0 0 0
Nucleus 0.15 0.18 0.16 0.05 0.09 0.09 0.08 0.08 0.05 0.1 0.09 0.12 0.06 0.19 0.13 0.17 0.09 0.13 0.06 0.08 0.09 0.08
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Nucleolus 0.94 0.91 0.8 0.88 0.87 0.86 0.86 0.83 0.87 0.8 0.86 0.82 0.81 0.82 0.85 0.84 0.84 0.79 0.82 0.37 0.52 0.44
Peroxisomes 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.14 0.12 0.19 0.24 0.27 0.28 0.29 0.26 0.18 0.23 0 0 0 0 0 0 0.06 0.22 0.12 0.14
Vac/Vac Membrane 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 3 1 5 4 2 0 1 0 0 1 3 0 0 0 0
Bud Neck 2 3 1 2 1 1 2 6 6 4 5 3 1 0 1 3 0 0 0 0 0 0
Bud Site 1 1 0 1 0 6 3 1 5 9 4 10 0 2 0 2
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 0 1 3 10 8 8 10 16 2 6 9 7 1 11 3 7 0 0 1 0 0 1
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0
Endosome 0 2 0 2 11 20 20 19 14 19 16 14 0 3 3 3 0 2 0 1 0 0
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0
Mitochondria 6 8 1 2 1 9 7 14 18 21 34 15 1 23 20 20 2 2 0 0 0 0
Nucleus 30 58 12 7 18 20 22 24 11 27 25 38 1 49 26 39 18 45 5 1 1 3
Nuclear Periphery 0 1 0 0 0 1 1 1 3 4 0 0 0 0 2 0 0 0 0 0 1 2
Nucleolus 187 289 59 126 180 191 228 250 197 224 228 265 13 209 170 192 170 268 75 4 12 18
Peroxisomes 0 1 0 1 3 12 2 3 5 2 5 3 0 1 1 1 0 0 0 0 0 0
SpindlePole 6 14 10 17 39 54 72 84 66 73 49 73 0 1 4 6 6 11 5 2 2 5
Vac/Vac Membrane 1 3 4 1 2 3 1 1 1 2 0 2 0 4 5 6 2 2 0 0 0 0
Unique Cell Count 198 317 74 143 208 223 264 301 226 279 265 324 16 254 199 228 204 340 91 13 23 41
Labelled Cell Count 233 381 90 169 265 325 369 422 329 396 379 432 17 308 236 280 204 340 91 13 23 41


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.6 16.3 15.9 14.4 13.2 11.3 10.4 10.1 9.0 9.0 8.4 8.9 11.2 26.3 27.1 24.1
Std Deviation (1e-4) 3.6 4.9 4.6 5.2 3.0 3.1 2.7 2.4 2.8 2.7 2.7 2.4 4.3 11.5 11.2 12.9
Intensity Change (Log2) -0.14 -0.26 -0.5 -0.61 -0.66 -0.83 -0.82 -0.92 -0.84 -0.51 0.73 0.77 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2.9 0 0 2.2 2.4 2.2
Nucleus -2.8 -1.8 -1.7 -2.0 -2.2 -3.2 -1.6 -1.7 -1.1 0 0.6 -0.7 0.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.7 1.4 1.2 1.4 0.7 1.6 0.1 1.3 0.4 0.1 0.5 1.1 0.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -0.3 1.0 1.9 2.4 2.6 2.7 2.3 1.0 1.7 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Utp18-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available