Standard name
Description Essential non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.11 0 0 0.05 0.12 0.1 0.12 0.12 0.1 0.1 0.14 0.07 0.13 0.17 0.11 0 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.89 0.76 0.85 0.87 0.67 0.35 0.38 0.36 0.46 0.46 0.48 0.48 0.72 0.75 0.71 0.52 0.67 0.57 0.72 0.79 0.76 0.77 0.73 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0.07 0 0 0 0 0 0 0.08 0.08 0.1 0.07 0 0 0 0 0 0 0.13 0.05 0.11 0.09 0.08 0.14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.1 0.14 0.1 0.26 0.53 0.5 0.51 0.38 0.38 0.29 0.36 0.21 0.12 0.12 0.32 0.27 0.32 0.11 0.09 0.05 0.08 0.12 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 3 1 1 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 1 2 3 2 3 10
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 10 4 7 12 27 25 33 16 15 14 23 10 26 53 12 2 7 0 1 1 0 1 2
Endoplasmic Reticulum 0 0 0 0 2 1 1 2 0 0 2 0 0 0 0 2 2 4 0 0 0 0 0 1
Endosome 0 0 0 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 1 4 4
Golgi 0 0 2 1 6 12 11 13 5 1 1 3 3 3 2 13 3 0 0 0 0 0 0 2
Mitochondria 0 0 1 0 1 0 0 0 2 1 6 4 1 0 0 0 0 0 0 1 2 2 1 3
Nucleus 34 66 112 181 156 77 97 97 60 70 66 81 97 154 224 56 47 51 53 96 144 170 208 191
Nuclear Periphery 0 0 0 0 1 0 0 2 0 0 0 0 0 0 1 0 0 1 0 0 2 1 2 1
Nucleolus 3 6 1 3 2 1 9 9 10 12 14 11 2 2 3 2 1 3 9 6 20 20 23 47
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0
Vac/Vac Membrane 1 9 18 20 61 117 126 135 49 58 40 60 29 24 39 35 19 29 8 10 10 17 34 63
Unique Cell Count 38 87 131 209 233 220 253 267 130 153 137 169 135 206 314 108 70 90 74 121 190 223 286 332
Labelled Cell Count 39 91 138 212 241 239 272 292 146 160 145 182 143 209 323 120 75 95 74 121 190 223 286 332


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.2 28.9 25.4 23.5 24.7 25.4 24.2 23.0 21.3 19.4 19.3 19.3 29.7 28.6 30.2 37.3 35.5 35.0 26.4 25.7 27.0
Std Deviation (1e-4) 4.5 8.8 8.0 4.4 5.3 5.4 5.9 5.5 5.5 5.6 5.4 5.6 8.6 8.9 11.4 12.7 11.8 14.2 7.4 7.5 8.2
Intensity Change (Log2) -0.11 -0.04 0.0 -0.07 -0.14 -0.25 -0.38 -0.4 -0.39 0.23 0.17 0.25 0.56 0.49 0.46 0.06 0.02 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.9 2.9 2.4 3.0 2.8 2.3 2.3 3.2 1.6 3.0 4.0 2.5 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 1.8 0 0 0 0 0 0 0 0 0 3.3 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.3 -3.9 -9.2 -8.8 -9.2 -6.7 -7.0 -6.5 -6.7 -2.7 -2.4 -3.2 -5.7 -3.0 -4.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 2.8 2.8 3.4 2.5 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.2 2.8 7.3 6.9 7.1 4.4 4.6 3.1 4.3 1.7 -0.6 -0.4 3.5 2.3 3.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpn8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available