Standard name
Description Component of the spindle pole body outer plaque; acts through the mitotic exit network to specify asymmetric spindle pole body inheritance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0.06 – 0.1 0.12 0.1 0.1 0 0 0 0 0 0 0 0 0 0.06 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.06 0 0 0 0 0.05 0.07 – 0.09 0.09 0.11 0.08 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.06 0 0 0 – 0 0 0 0 0.07 0.08 0.12 0.08 0.06 0.08 0 0 0 0 0.09 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.06 0 0 0 0 0 – 0 0 0 0 0.05 0.11 0.13 0.06 0.11 0.07 0 0.05 0 0.07 0.08 0.06
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.07 0.07 0.11 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.06
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 0.07 0.05 0.07 0.08 0.07 0.05 – 0.05 0.07 0.08 0.05 0.09 0.08 0.05 0.09 0.18 0.14 0 0 0 0 0 0
Peroxisomes 0.1 0.1 0 0 0 0.06 0 – 0.05 0 0 0 0.09 0.19 0.22 0.19 0.15 0.14 0 0 0 0 0 0
SpindlePole 0.86 0.86 0.88 0.87 0.93 0.91 0.91 – 0.87 0.86 0.84 0.91 0.84 0.79 0.71 0.79 0.82 0.82 0.89 0.83 0.81 0.67 0.6 0.6
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 2 2 2 4 – 4 3 1 4 3 0 0 0 0 0 0 1 2 5 21 35
Bud 5 15 8 5 14 24 33 – 36 42 34 32 5 3 5 4 1 1 2 8 2 21 29 29
Bud Neck 3 12 7 4 5 5 13 – 6 9 8 5 3 3 4 1 2 1 3 9 3 9 16 26
Bud Site 18 16 11 6 4 20 39 – 31 33 37 24 14 13 9 15 6 11 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 2
Cytoplasm 10 11 16 14 11 9 22 – 13 15 10 6 26 51 75 40 22 25 3 1 3 15 66 100
Endoplasmic Reticulum 0 0 2 2 0 0 0 – 0 0 0 1 0 1 1 5 3 1 0 0 0 0 4 4
Endosome 7 30 4 2 3 5 5 – 11 0 6 4 17 71 84 29 40 20 10 27 13 25 56 53
Golgi 0 0 0 1 0 0 0 – 0 0 0 0 0 0 1 0 0 2 2 1 2 1 3 1
Mitochondria 12 20 2 4 1 7 14 – 26 26 37 11 11 5 10 13 2 5 0 4 3 20 52 49
Nucleus 4 7 3 7 10 3 6 – 3 5 2 3 0 3 3 6 7 2 2 1 0 0 0 0
Nuclear Periphery 0 0 2 0 0 1 0 – 0 0 0 0 1 0 0 0 1 0 1 0 0 0 2 1
Nucleolus 50 37 14 15 28 25 27 – 19 27 25 14 35 48 35 47 66 40 2 13 6 2 0 0
Peroxisomes 30 49 4 1 5 21 16 – 17 10 12 5 32 119 142 94 56 40 1 10 7 14 16 16
SpindlePole 255 425 234 194 325 338 487 – 301 311 272 277 313 495 461 402 306 243 270 442 238 245 423 510
Vac/Vac Membrane 1 3 2 1 0 0 1 – 0 0 2 2 3 6 8 6 5 3 0 1 2 1 5 9
Unique Cell Count 297 494 267 222 351 371 537 347 360 324 305 372 626 646 506 374 295 303 535 294 368 711 855
Labelled Cell Count 395 626 310 258 408 460 667 467 481 446 388 463 818 839 662 517 394 303 535 294 368 711 855


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 5.3 6.3 5.8 6.4 5.0 5.1 – 4.5 4.5 4.2 4.9 5.3 5.5 5.7 9.7 9.7 9.2 4.5 4.0 4.2
Std Deviation (1e-4) 1.0 0.8 1.5 1.7 1.8 1.4 1.5 – 1.2 1.2 1.2 1.1 1.1 1.3 1.5 2.2 2.6 2.5 1.2 1.2 1.1
Intensity Change (Log2) – – – -0.12 0.03 -0.32 -0.29 – -0.47 -0.49 -0.57 -0.35 -0.25 -0.18 -0.13 0.63 0.64 0.56 -0.49 -0.63 -0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud -0.5 0.7 2.0 1.9 – 0 0 0 3.5 -1.5 0 0 0 0 0
Bud Neck 0 -1.1 -1.2 -0.2 – 0 0 0 -0.8 0 0 0 0 0 0
Bud Site -0.9 -2.4 0.7 1.7 – 0 0 0 1.9 -0.2 -1.7 -2.6 -0.8 -2.0 -0.2
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 -1.7 -2.3 -1.2 – -1.3 -1.0 -1.7 -2.5 0.5 1.1 2.6 1.0 -0.1 1.1
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 2.1 4.9 5.4 2.8 4.5 3.1
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 1.8 – 0 0 0 2.3 1.9 0 0 1.7 0 0
Nucleus 0 1.5 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.7 1.3 0.8 -0.1 – 0 0 0 -0.4 2.0 1.3 0.1 2.0 4.7 3.3
Peroxisomes 0 0 2.7 1.3 – 0 0 0 0 3.8 7.0 7.7 6.8 5.8 5.3
SpindlePole -0.1 2.1 1.4 1.3 – 0 0 0 1.2 -1.2 -3.0 -5.2 -2.8 -2.0 -1.7
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0