Standard name
Description Component of the mitochondrial TIM22 complex; involved in insertion of polytopic proteins into the inner membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.1 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.1 0.15 – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0.1 0.15 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0.05 0.06 0.05 0 – 0 0 0 0 0 0
Endosome 0 0 0.05 0 0.08 0 0 0 – 0 0 0 0 0 0 0.05 0.05 – 0 0 0 0.06 0 0
Golgi 0.05 0 0 0 0 0 0 0 – 0 0 0 0.29 0.27 0.42 0.05 0.25 – 0 0.08 0 0 0.06 0.07
Mitochondria 0.84 0.93 0.88 0.95 0.8 0.9 0.87 0.92 – 0.93 0.94 0.95 0.55 0.54 0.44 0.05 0.25 – 0.81 0.75 0.76 0.66 0.69 0.7
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.15 0 – 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0.06 0.05 0 0.06 0.09 0.11 – 0.05 0.08 0.08 0 0 0 0.05 0.05 – 0 0 0 0 0 0
Nucleolus 0.09 0 0.06 0 0.05 0.11 0.12 0.1 – 0.08 0.07 0.06 0 0 0 0.1 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0.05 0.06 0 0 0 – 0 0 0 0 0 0 0.05 0.05 – 0 0 0.06 0.09 0.07 0.06
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.2 0.05 – 0 0 0 0 0 0
Vac/Vac Membrane 0.21 0.12 0.1 0 0.13 0.07 0.07 0 – 0 0 0 0.26 0.28 0.29 0.15 0.35 – 0 0.06 0 0 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 0 0 0 0 0 1 – 0 0 0 5 6 9 0 0 – 2 4 9 9 8 14
Bud 2 4 6 3 4 4 13 11 – 10 4 11 3 3 1 0 0 – 0 2 0 3 3 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 1 2 1 3 1 1
Bud Site 1 1 0 1 0 1 5 4 – 3 6 3 1 1 0 2 0 – – – – – – –
Cell Periphery 2 9 8 1 1 0 2 0 – 1 2 1 10 10 9 2 3 – 0 0 2 0 0 0
Cytoplasm 1 2 6 3 12 9 11 7 – 8 8 9 10 10 11 2 3 – 0 1 2 2 0 0
Endoplasmic Reticulum 2 11 4 0 0 0 0 5 – 0 3 5 12 13 18 1 0 – 0 0 1 1 3 5
Endosome 5 0 16 5 20 0 4 2 – 0 0 0 8 8 11 1 1 – 6 12 14 16 16 16
Golgi 9 4 2 2 1 0 0 0 – 0 0 0 74 71 120 1 5 – 9 23 15 11 26 30
Mitochondria 162 270 294 192 207 201 361 308 – 225 213 305 141 141 124 1 5 – 163 223 264 180 283 292
Nucleus 0 2 2 1 3 0 9 4 – 4 3 4 4 4 1 3 0 – 0 0 0 0 1 0
Nuclear Periphery 10 10 19 10 8 14 37 35 – 11 18 26 0 1 1 1 1 – 2 1 5 3 7 3
Nucleolus 17 8 20 8 13 25 51 32 – 19 17 20 0 0 1 2 0 – 1 1 1 2 2 1
Peroxisomes 3 0 23 10 15 1 9 4 – 1 0 4 3 3 1 1 1 – 8 3 22 23 27 24
SpindlePole 5 1 5 5 11 0 13 9 – 4 4 7 3 2 5 4 1 – 3 2 2 2 2 0
Vac/Vac Membrane 41 36 34 7 34 15 28 11 – 6 9 10 67 73 83 3 7 – 2 17 3 9 23 17
Unique Cell Count 193 291 336 203 259 223 417 333 243 227 321 258 261 285 20 20 203 298 349 273 413 418
Labelled Cell Count 260 361 439 248 329 270 543 433 292 287 405 341 346 395 24 27 203 298 349 273 413 418


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.2 7.5 6.9 6.4 6.5 5.3 4.9 4.9 – 4.2 4.2 4.1 7.9 7.9 8.1 6.9 9.6 – 8.3 8.6 9.1
Std Deviation (1e-4) 1.5 1.3 1.9 1.4 1.7 1.7 1.2 1.0 – 1.2 1.3 1.0 1.1 1.1 0.9 1.4 2.4 – 2.0 1.6 1.7
Intensity Change (Log2) – – – -0.1 -0.09 -0.37 -0.5 -0.49 – -0.71 -0.73 -0.76 0.2 0.2 0.22 0.01 0.48 – 0.26 0.32 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 1.2 – 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 0 0 – 0 0 0 1.1 1.0 0.6 0 0 –
Cytoplasm 0 2.0 1.6 0.8 0.3 – 1.2 1.3 0.9 1.6 1.5 1.6 0 0 –
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 2.6 2.8 3.4 0 0 –
Endosome -1.3 1.5 -3.3 0 -3.3 – 0 0 0 -1.0 -1.0 -0.5 0 0 –
Golgi 0 0 0 0 0 – 0 0 0 10.2 9.8 13.0 0 0 –
Mitochondria 2.7 -2.5 1.0 0 2.2 – 0 0 0 -9.0 -9.1 -11.6 -9.5 -7.4 –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 –
Nuclear Periphery -0.4 -1.5 0.3 0 2.3 – 0 0 0 -3.9 -3.6 -3.7 0 0 –
Nucleolus -1.0 -0.5 2.2 0 1.8 – 0 0 0 -4.0 -4.0 -3.8 0 0 –
Peroxisomes -0.9 -0.5 -3.7 0 -3.7 – 0 0 0 -3.4 -3.4 -4.2 0 0 –
SpindlePole 0 2.1 0 0 1.1 – 0 0 0 0 0 0 0 0 –
Vacuole -2.8 1.1 -1.4 -1.7 -3.5 – -3.6 -2.7 -3.6 5.1 5.6 6.0 0 0 –