Standard name
Description Core component of the signal recognition particle (SRP); the SRP is a ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.08 0.07 0 0 0 0.07 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.09 0.14 0.15 0.07 0.09 0.08 0.07 0.07 0.1 0.12 0.28 0.31 0.28 0.34 0.28 0.4 0 0 0 0 0 0
Cytoplasm 0.47 0.41 0.39 0.35 0.37 0.45 0.45 0.56 0.57 0.57 0.53 0.55 0.63 0.68 0.65 0.68 0.62 0.11 0.15 0.14 0.21 0.27 0.23
Endoplasmic Reticulum 0.56 0.68 0.58 0.58 0.58 0.53 0.51 0.31 0.37 0.24 0.33 0.46 0.36 0.36 0 0 0 0.84 0.78 0.79 0.61 0.66 0.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.08 0 0.19 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.13 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 10 8 21 19 11 11 5 19 11 21 1 1 0 6 2 9 1 6 3 3 3 2
Bud 1 1 0 2 4 4 3 5 2 8 5 0 0 0 0 0 1 1 3 0 0 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 2 2 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 7 38 32 39 19 24 28 12 21 24 35 28 98 73 87 70 103 0 5 6 2 1 2
Cytoplasm 85 175 92 91 94 124 164 97 165 139 159 55 198 175 165 169 162 21 67 36 26 83 71
Endoplasmic Reticulum 101 289 137 149 147 148 184 54 108 58 98 46 112 92 1 9 1 159 354 209 79 201 218
Endosome 0 0 0 1 2 1 2 0 1 0 0 0 0 0 0 2 0 0 1 0 0 1 3
Golgi 1 1 1 1 2 4 2 0 0 0 0 0 0 0 13 9 5 0 1 0 0 0 1
Mitochondria 1 0 0 1 0 6 12 13 8 47 20 0 0 0 2 2 1 0 1 2 3 1 2
Nucleus 0 0 2 1 2 1 1 1 2 1 1 0 1 0 0 3 2 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 0 0 0 0 0 2 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 1 1 2 0 5 2 11 3 11 8 7 0 0 3 16 32 13 1 4 1 3 3 3
Unique Cell Count 179 423 237 259 254 278 363 173 289 246 298 100 314 258 255 249 260 191 452 265 129 307 313
Labelled Cell Count 201 515 274 306 294 325 419 191 337 297 346 130 410 343 291 298 298 191 452 265 129 307 313


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.0 19.1 15.2 13.6 12.5 11.7 11.0 9.6 10.0 9.2 9.4 14.8 14.4 13.7 17.7 17.3 17.4 13.5 13.9 14.5
Std Deviation (1e-4) 2.4 2.7 1.9 1.8 1.9 1.4 1.8 1.5 1.5 1.4 1.5 1.8 1.8 1.7 3.3 3.8 3.1 2.3 2.2 2.4
Intensity Change (Log2) -0.16 -0.28 -0.38 -0.47 -0.66 -0.61 -0.72 -0.69 -0.04 -0.07 -0.15 0.22 0.19 0.2 -0.17 -0.13 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.2 2.0 0.3 -0.2 -0.3 1.7 0.6 1.9 0 0 0 -0.7 0 0.1
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.5 -2.2 -1.8 -2.3 -2.1 -2.4 -1.3 -0.6 3.2 4.8 4.0 5.3 4.0 6.5
Cytoplasm -0.8 -0.4 1.3 1.5 3.5 4.2 3.9 3.3 2.7 5.6 6.5 5.7 6.4 5.2
Endoplasmic Reticulum -0.1 0 -1.0 -1.7 -5.3 -4.7 -7.7 -5.8 -2.0 -5.2 -4.9 -14.2 -13.0 -14.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 3.1 0 0
Mitochondria 0 0 0 0 4.3 0 7.1 4.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 1.8 0 2.2 0 0 0 0 0 3.2 5.2 2.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Srp72-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available