Standard name
Description DNA damage response kinase; signal transduction pathway component required for DNA damage and replication checkpoints, promoting cell cycle arrest and DNA repair; role in initiation of DNA replication; inhibits gene gating through NPC protein phosphorylation, to promote fork stability; activates downstream kinase Dun1p; senses mtDNA depletion and mitochondrial ROS; relocalizes to cytosol under hypoxia; contains two FHA domains; human homolog CHEK2 implicated in breast cancer complements the null

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.10 0.13 0.06 0.10 0.05 0.07 0.07 0.05 0 0.08 0.14 0.14 0.10 0.07 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.08 0.08 0.17 0.07 0.24 0.28 0.22 0.47 0.50 0.27 0.05 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.96 0.88 0.85 0.79 0.88 0.83 0.86 0.83 0.80 0.76 0.86 0.88 0.84 0.82 0.84 0.88 0.87 0.88 0.83 0.73 0.66 0.58 0.51
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.08 0.06 0.07 0.16 0.24 0.27 0.30
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 1 0
Bud 0 1 1 0 1 2 5 8 12 7 3 0 0 0 1 0 0 0 7 6 1 9 7
Bud Neck 0 0 3 0 0 1 4 2 2 1 0 0 0 0 0 0 0 0 0 2 0 3 5
Bud Site 0 0 0 0 4 12 24 8 20 20 29 0 1 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 2 20 43 26 21 54 32 39 33 21 14 7 60 48 25 21 13 1 6 13 2 6 4
Endoplasmic Reticulum 0 1 1 0 0 0 0 0 0 0 1 0 0 1 3 4 8 0 0 1 0 0 3
Endosome 0 0 1 1 3 0 0 1 0 0 1 0 1 2 3 1 1 1 5 2 0 2 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 1 2
Mitochondria 10 31 34 35 23 128 174 118 239 204 122 4 7 5 4 1 2 1 6 7 0 7 7
Nucleus 111 334 358 157 288 441 536 440 404 312 393 74 369 278 218 247 204 107 333 363 69 212 154
Nuclear Periphery 0 1 0 1 2 2 2 5 10 5 4 0 0 3 3 2 0 0 1 0 0 1 1
Nucleolus 0 0 0 0 0 1 3 5 14 25 12 2 1 5 0 1 2 0 0 1 0 9 6
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 1 6 6 7 4 11 4 3 5 1 3 2 0 0 0 1 6 11 1 7 6
Vac/Vac Membrane 5 18 10 3 13 2 5 3 1 3 6 0 6 13 14 14 18 6 27 77 24 100 91
Unique Cell Count 116 380 421 200 328 530 622 531 504 409 457 84 441 340 260 281 234 122 400 500 105 369 303
Labelled Cell Count 128 407 452 229 361 650 789 640 739 602 590 89 450 357 272 291 250 122 400 500 105 369 303

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.1 5.2 5.1 5.8 4.7 4.8 5.0 4.3 4.0 4.8 5.2 5.4 5.4 8.7 9.1 8.6 4.5 4.6 5.1
Std Deviation (1e-4) 0.6 0.8 1.1 1.5 1.4 1.4 1.1 1.2 1.1 1.0 1.1 1.5 1.5 1.5 1.9 1.9 1.9 1.0 1.3 1.8
Intensity Change (Log2) -0.0 0.2 -0.1 -0.1 -0.0 -0.3 -0.4 -0.1 -0.0 0.1 0.1 0.8 0.8 0.7 -0.2 -0.2 -0.0

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 3.0 0 0 3.9 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.5 -0.5 0 0 5.9 0 0 0 -1.1 -4.5 -4.1 -3.6 -4.6 -3.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2.5 0 0 0 0 0 0 0 0 0
Vacuole 0 0.8 0 0 -2.4 0 0 0 0 -1.1 0.7 0.3 0.4 2.1