Standard name
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0 0.06 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.98 0.97 0.95 0.88 0.81 0.8 0.78 0.75 0.79 0.75 0.97 0.98 0.98 0.98 0.96 1.0 0.93 0.94 0.93 0.92 0.94 0.96
Endoplasmic Reticulum 0 0 0 0 0.05 0.08 0.14 0.16 0.16 0.18 0.13 0.16 0 0 0 0 0.09 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 6 10 15 19 21 9 20 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 2 1 2 1 1 2 3 1 2 1 3 0 1 2 0 0 0 1 1 1 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 11 13 16 28 20 31 25 9 19 3 7 10 1 0 0 0 0 0 0 0 0
Cytoplasm 209 210 270 353 520 380 505 486 396 340 234 293 188 255 308 50 44 34 190 207 260 115 305 347
Endoplasmic Reticulum 2 0 9 7 25 34 84 98 83 84 40 64 3 4 2 1 4 3 4 2 8 3 8 9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 1 0 0 0 11 17 7 11 14 2 13 1 1 2 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 1 1 0 1 7 6 10 5 0 0 0 0 0 1 1 1 2 0 1 2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 3 4 6 13 8 12 13 11 1 0 1 0 1 0 0 2 1 1 2 0
Unique Cell Count 211 211 276 363 546 430 622 607 509 455 298 389 193 260 313 51 46 34 205 222 280 125 326 364
Labelled Cell Count 213 214 281 374 563 453 652 643 556 504 318 429 196 268 326 52 49 38 205 222 280 125 326 364


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 60.9 97.4 73.8 68.4 55.0 42.5 35.6 32.6 33.3 30.3 27.2 31.2 72.0 76.9 74.2 186.2 227.6 194.5 78.1 82.5 91.1
Std Deviation (1e-4) 15.3 16.4 18.7 19.0 12.6 11.9 8.7 8.7 8.6 8.2 8.5 9.0 22.6 21.2 22.2 63.1 75.2 93.1 23.9 23.7 24.6
Intensity Change (Log2) – – – -0.11 -0.42 -0.79 -1.05 -1.18 -1.15 -1.28 -1.44 -1.24 -0.04 0.06 0.01 1.34 1.63 1.4 0.08 0.16 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 3.2 3.6 0 3.8 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 3.2 3.6 3.1 4.2 4.0 0 3.7 0 0 0 0 0 0
Cytoplasm -0.5 -1.8 -4.5 -6.7 -7.0 -7.5 -8.1 -7.1 -7.9 -0.3 0.2 0.5 0.1 -0.9 0.9
Endoplasmic Reticulum -1.1 0.9 2.5 4.6 5.4 5.4 6.0 4.4 5.4 0 -1.3 -2.3 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 2.8 0 0 2.9 0 3.1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0 0