Standard name | |
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Description | Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 6 | 10 | 15 | 19 | 21 | 9 | 20 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 2 | 1 | 2 | 1 | 1 | 2 | 3 | 1 | 2 | 1 | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 0 | 11 | 13 | 16 | 28 | 20 | 31 | 25 | 9 | 19 | 3 | 7 | 10 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 209 | 210 | 270 | 353 | 520 | 380 | 505 | 486 | 396 | 340 | 234 | 293 | 188 | 255 | 308 | 50 | 44 | 34 | 190 | 207 | 260 | 115 | 305 | 347 |
Endoplasmic Reticulum | 2 | 0 | 9 | 7 | 25 | 34 | 84 | 98 | 83 | 84 | 40 | 64 | 3 | 4 | 2 | 1 | 4 | 3 | 4 | 2 | 8 | 3 | 8 | 9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 1 | 0 | 0 | 0 | 11 | 17 | 7 | 11 | 14 | 2 | 13 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 7 | 6 | 10 | 5 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 1 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 1 | 3 | 4 | 6 | 13 | 8 | 12 | 13 | 11 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 2 | 0 |
Unique Cell Count | 211 | 211 | 276 | 363 | 546 | 430 | 622 | 607 | 509 | 455 | 298 | 389 | 193 | 260 | 313 | 51 | 46 | 34 | 205 | 222 | 280 | 125 | 326 | 364 |
Labelled Cell Count | 213 | 214 | 281 | 374 | 563 | 453 | 652 | 643 | 556 | 504 | 318 | 429 | 196 | 268 | 326 | 52 | 49 | 38 | 205 | 222 | 280 | 125 | 326 | 364 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 60.9 | 97.4 | 73.8 | 68.4 | 55.0 | 42.5 | 35.6 | 32.6 | 33.3 | 30.3 | 27.2 | 31.2 | 72.0 | 76.9 | 74.2 | 186.2 | 227.6 | 194.5 | 78.1 | 82.5 | 91.1 |
Std Deviation (1e-4) | 15.3 | 16.4 | 18.7 | 19.0 | 12.6 | 11.9 | 8.7 | 8.7 | 8.6 | 8.2 | 8.5 | 9.0 | 22.6 | 21.2 | 22.2 | 63.1 | 75.2 | 93.1 | 23.9 | 23.7 | 24.6 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.42 | -0.79 | -1.05 | -1.18 | -1.15 | -1.28 | -1.44 | -1.24 | -0.04 | 0.06 | 0.01 | 1.34 | 1.63 | 1.4 | 0.08 | 0.16 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 3.2 | 3.6 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 3.2 | 3.6 | 3.1 | 4.2 | 4.0 | 0 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | -1.8 | -4.5 | -6.7 | -7.0 | -7.5 | -8.1 | -7.1 | -7.9 | -0.3 | 0.2 | 0.5 | 0.1 | -0.9 | 0.9 |
Endoplasmic Reticulum | -1.1 | 0.9 | 2.5 | 4.6 | 5.4 | 5.4 | 6.0 | 4.4 | 5.4 | 0 | -1.3 | -2.3 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 2.8 | 0 | 0 | 2.9 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |