Standard name
Description Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease (Sen complex) is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen complex also cleaves the CBP1 mRNA at the mitochondrial surface
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.14 0.26 0 0 0 0 0.05 0.08
Bud 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.13 0.09 0 0 0 0 0 0
Cytoplasm 0.23 0.19 0.11 0.36 0.26 0.2 0.14 0.15 0.15 0.09 0.08 0.14 0.56 0.54 0.58 0.46 0.31 0.29 0 0 0 0.07 0 0.05
Endoplasmic Reticulum 0 0.12 0.06 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.08 0.05 0.06 0.05 0 0 0 0 0
Endosome 0.14 0 0.06 0.05 0.06 0 0 0 0 0 0 0 0 0.1 0.07 0.1 0.15 0.07 0.13 0.15 0.06 0.11 0.11 0.12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.07 0 0 0 0.05
Mitochondria 0.66 0.54 0.82 0.68 0.62 0.79 0.84 0.83 0.78 0.9 0.91 0.88 0.22 0.23 0.22 0.09 0.12 0.12 0.54 0.51 0.61 0.57 0.54 0.43
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0 0
Vac/Vac Membrane 0.2 0.16 0.08 0.08 0.12 0.1 0.11 0.08 0.12 0.12 0 0.05 0.17 0.14 0.14 0.16 0.2 0.19 0.07 0.1 0.08 0.08 0.06 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 12 0 1 1 0 0 0 0 0 0 0 1 2 0 26 32 59 5 5 4 4 14 19
Bud 2 1 3 4 3 5 5 18 4 7 7 9 1 4 4 11 1 16 2 4 0 2 5 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 2 1 1 7 11
Bud Site 1 0 0 0 0 1 1 6 3 0 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 7 1 1 4 1 2 4 2 0 2 2 6 7 9 29 30 21 1 0 1 0 0 1
Cytoplasm 33 38 10 47 37 37 41 55 26 19 17 29 88 130 143 145 71 65 6 8 2 11 10 11
Endoplasmic Reticulum 0 24 5 3 6 2 0 2 4 1 4 1 8 12 3 24 11 14 7 9 2 2 5 8
Endosome 20 8 5 6 8 2 5 7 1 1 0 0 6 25 18 32 35 15 20 36 7 16 31 29
Golgi 0 8 1 0 2 0 0 0 0 0 1 0 4 5 4 2 6 15 10 17 4 4 11 12
Mitochondria 95 108 71 88 89 146 239 301 131 182 186 189 35 55 54 30 28 27 83 128 67 86 151 104
Nucleus 0 3 0 0 2 1 7 8 4 1 2 1 0 1 0 6 4 3 0 0 0 0 0 0
Nuclear Periphery 0 2 0 0 2 2 2 7 3 2 4 1 0 0 1 0 3 1 0 0 0 1 2 2
Nucleolus 0 0 0 0 0 2 2 2 0 3 2 1 0 0 0 0 2 0 0 0 1 0 1 0
Peroxisomes 0 1 0 0 0 0 2 2 0 1 1 1 0 2 4 1 4 0 0 1 1 0 4 5
SpindlePole 1 0 1 0 1 3 7 17 1 4 5 8 0 1 0 19 18 10 1 4 1 2 3 4
Vac/Vac Membrane 28 32 7 10 18 18 32 30 20 24 8 10 27 35 34 50 46 42 11 25 8 12 16 21
Unique Cell Count 143 200 87 130 144 184 285 361 168 202 204 214 158 242 245 318 226 227 155 250 112 152 281 244
Labelled Cell Count 183 244 104 160 173 220 345 459 199 245 241 254 176 279 274 377 292 288 155 250 112 152 281 244


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.6 5.1 5.2 5.2 4.6 4.0 4.2 4.2 3.4 3.5 3.7 6.4 6.2 5.9 5.9 7.3 7.7 5.7 6.0 6.6
Std Deviation (1e-4) 0.7 0.7 1.7 1.6 1.3 1.5 1.5 1.3 1.5 1.1 0.9 1.4 1.2 1.4 1.1 1.1 1.3 1.3 1.3 1.7 1.5
Intensity Change (Log2) – – – 0.04 0.05 -0.15 -0.33 -0.25 -0.28 -0.57 -0.54 -0.44 0.35 0.28 0.21 0.21 0.53 0.6 0.18 0.26 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 2.8 3.7 5.3
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.5 3.2 2.5
Cytoplasm 4.0 2.6 1.7 0.7 0.9 0.9 -0.5 -0.9 0.5 6.8 6.8 7.5 5.8 3.6 3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.6 0 0.1
Endosome 0 0 0 0 0 0 0 0 0 0 1.3 0.5 1.2 2.3 0.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.3 -3.2 0 0 0 0 0 0 0 -9.0 -9.7 -9.9 -13.8 -11.8 -11.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 2.3 0
Vacuole -0.1 1.1 0.5 0.8 0.1 0.9 1.0 0 0 2.0 1.5 1.4 1.8 2.6 2.3