| Standard name | |
|---|---|
| Description | ATPase involved in protein import into the ER; also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
| Bud | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 4 | 6 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 6 | 1 |
| Bud Neck | 1 | 0 | 2 | 0 | 3 | 1 | 1 | – | 0 | 5 | 2 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 2 | 1 | 2 | 0 | 1 | 1 | 0 | – | 1 | 1 | 3 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
| Cytoplasm | 174 | 309 | 97 | 115 | 151 | 206 | 238 | – | 126 | 168 | 213 | 237 | 250 | 226 | 242 | 25 | 66 | 35 | 167 | 289 | 94 | 126 | 204 | 180 |
| Endoplasmic Reticulum | 1 | 2 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 1 | 3 | 0 | 1 | 1 | 2 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 2 |
| Endosome | 0 | 1 | 0 | 0 | 0 | 3 | 0 | – | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 1 | 5 | 0 | 0 | 0 | 0 |
| Golgi | 0 | 0 | 0 | 0 | 2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 0 |
| Mitochondria | 4 | 0 | 0 | 1 | 0 | 3 | 1 | – | 26 | 19 | 65 | 37 | 0 | 1 | 0 | 1 | 1 | 3 | 4 | 8 | 4 | 1 | 2 | 4 |
| Nucleus | 0 | 0 | 1 | 1 | 3 | 2 | 0 | – | 1 | 1 | 2 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 0 | 1 | 0 | 1 | 0 | 0 | 1 | – | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 0 | 0 | 0 | 1 | 1 | 1 | 4 | – | 2 | 2 | 1 | 8 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 |
| Vac/Vac Membrane | 0 | 2 | 0 | 1 | 1 | 6 | 5 | – | 4 | 4 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 5 | 3 | 1 | 1 | 1 |
| Unique Cell Count | 179 | 314 | 97 | 120 | 157 | 217 | 245 | 148 | 188 | 252 | 280 | 251 | 228 | 244 | 26 | 74 | 41 | 184 | 326 | 113 | 138 | 225 | 200 | |
| Labelled Cell Count | 182 | 316 | 103 | 120 | 163 | 226 | 251 | 168 | 209 | 298 | 312 | 251 | 229 | 245 | 30 | 78 | 45 | 184 | 326 | 113 | 138 | 225 | 200 | |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 3.5 | 4.6 | 5.1 | 5.2 | 6.2 | 5.0 | 4.9 | – | 4.3 | 4.4 | 3.9 | 4.2 | 5.2 | 5.2 | 5.4 | 6.2 | 6.0 | 5.8 | 4.9 | 5.0 | 5.0 |
| Std Deviation (1e-4) | 0.5 | 1.2 | 1.2 | 3.2 | 2.7 | 2.4 | 2.1 | – | 1.7 | 1.7 | 1.9 | 2.2 | 1.6 | 1.0 | 2.1 | 2.4 | 1.9 | 2.8 | 1.5 | 1.9 | 1.3 |
| Intensity Change (Log2) | – | – | – | 0.02 | 0.28 | -0.05 | -0.07 | – | -0.26 | -0.24 | -0.41 | -0.3 | 0.02 | 0.0 | 0.07 | 0.26 | 0.21 | 0.18 | -0.08 | -0.03 | -0.05 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Kar2-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
