Standard name | |
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Human Ortholog | |
Description | ATPase involved in protein import into the ER; also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 4 | 6 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 6 | 1 |
Bud Neck | 1 | 0 | 2 | 0 | 3 | 1 | 1 | – | 0 | 5 | 2 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 2 | 0 | 1 | 1 | 0 | – | 1 | 1 | 3 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Cytoplasm | 174 | 309 | 97 | 115 | 151 | 206 | 238 | – | 126 | 168 | 213 | 237 | 250 | 226 | 242 | 25 | 66 | 35 | 167 | 289 | 94 | 126 | 204 | 180 |
Endoplasmic Reticulum | 1 | 2 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 1 | 3 | 0 | 1 | 1 | 2 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 2 |
Endosome | 0 | 1 | 0 | 0 | 0 | 3 | 0 | – | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 1 | 5 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 0 |
Mitochondria | 4 | 0 | 0 | 1 | 0 | 3 | 1 | – | 26 | 19 | 65 | 37 | 0 | 1 | 0 | 1 | 1 | 3 | 4 | 8 | 4 | 1 | 2 | 4 |
Nucleus | 0 | 0 | 1 | 1 | 3 | 2 | 0 | – | 1 | 1 | 2 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 1 | 0 | 0 | 1 | – | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 1 | 1 | 4 | – | 2 | 2 | 1 | 8 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 2 | 0 | 1 | 1 | 6 | 5 | – | 4 | 4 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 5 | 3 | 1 | 1 | 1 |
Unique Cell Count | 179 | 314 | 97 | 120 | 157 | 217 | 245 | 148 | 188 | 252 | 280 | 251 | 228 | 244 | 26 | 74 | 41 | 184 | 326 | 113 | 138 | 225 | 200 | |
Labelled Cell Count | 182 | 316 | 103 | 120 | 163 | 226 | 251 | 168 | 209 | 298 | 312 | 251 | 229 | 245 | 30 | 78 | 45 | 184 | 326 | 113 | 138 | 225 | 200 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 4.6 | 5.1 | 5.2 | 6.2 | 5.0 | 4.9 | – | 4.3 | 4.4 | 3.9 | 4.2 | 5.2 | 5.2 | 5.4 | 6.2 | 6.0 | 5.8 | 4.9 | 5.0 | 5.0 |
Std Deviation (1e-4) | 0.5 | 1.2 | 1.2 | 3.2 | 2.7 | 2.4 | 2.1 | – | 1.7 | 1.7 | 1.9 | 2.2 | 1.6 | 1.0 | 2.1 | 2.4 | 1.9 | 2.8 | 1.5 | 1.9 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.28 | -0.05 | -0.07 | – | -0.26 | -0.24 | -0.41 | -0.3 | 0.02 | 0.0 | 0.07 | 0.26 | 0.21 | 0.18 | -0.08 | -0.03 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.0037 | -1.0578 | -1.0971 | -1.5791 | -1.5261 | -1.0419 | -0.0294 | -0.5796 | -0.507 | -0.4687 | -0.6827 | -0.6327 | -0.8545 | -1.0562 | -0.8243 | -0.912 | -0.8829 | -1.0972 |
Actin | 0.0448 | 0.0001 | 0.0003 | 0.0311 | 0.0002 | 0.0072 | 0.0821 | 0.0134 | 0.0172 | 0.0076 | 0.0041 | 0.0091 | 0.0316 | 0.0012 | 0.0123 | 0.0003 | 0.0081 | 0.0083 |
Bud | 0.0005 | 0.0003 | 0.0102 | 0.0003 | 0 | 0.0007 | 0.0082 | 0.0033 | 0.0082 | 0.0069 | 0.0022 | 0.0041 | 0.0006 | 0.0017 | 0.0062 | 0.0002 | 0.0003 | 0.0004 |
Bud Neck | 0.0008 | 0.0003 | 0.0001 | 0.0021 | 0 | 0.0022 | 0.0036 | 0.0006 | 0.0097 | 0.0165 | 0.0014 | 0.0003 | 0.0017 | 0.0002 | 0.0002 | 0 | 0.0005 | 0.0006 |
Bud Periphery | 0.0005 | 0.0002 | 0.0026 | 0.0004 | 0 | 0.0018 | 0.0042 | 0.0042 | 0.0042 | 0.0044 | 0.0025 | 0.0074 | 0.0008 | 0.0022 | 0.0014 | 0.0004 | 0.0003 | 0.0015 |
Bud Site | 0.0032 | 0.0109 | 0.0024 | 0.012 | 0 | 0.0005 | 0.0108 | 0.028 | 0.0188 | 0.0475 | 0.0186 | 0.0005 | 0.0024 | 0.0128 | 0.0047 | 0.0001 | 0.0005 | 0.0002 |
Cell Periphery | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0005 | 0.0003 | 0.0006 | 0.0009 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Cytoplasm | 0.0231 | 0.0361 | 0.0386 | 0.0313 | 0.094 | 0.0694 | 0.0186 | 0.0202 | 0.0171 | 0.0204 | 0.0366 | 0.049 | 0.0489 | 0.0487 | 0.0499 | 0.0133 | 0.0427 | 0.0706 |
Cytoplasmic Foci | 0.0588 | 0.0324 | 0.0215 | 0.0294 | 0.0016 | 0.0185 | 0.0369 | 0.0036 | 0.0187 | 0.0504 | 0.0084 | 0.0126 | 0.0595 | 0.0065 | 0.0282 | 0.0046 | 0.0368 | 0.0102 |
Eisosomes | 0.002 | 0.0001 | 0.0004 | 0.0006 | 0.0012 | 0.0002 | 0.0006 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0039 | 0.0003 | 0.0006 | 0.0001 | 0.0004 | 0.0043 | 0.0115 | 0.0007 | 0.0004 | 0.0007 | 0.002 | 0.0009 | 0.0012 | 0.0006 | 0.0014 | 0.0002 | 0.0006 | 0.0002 |
Endosome | 0.0746 | 0.005 | 0.0101 | 0.0041 | 0.0002 | 0.0259 | 0.0473 | 0.0005 | 0.0092 | 0.0157 | 0.0138 | 0.0052 | 0.0422 | 0.0007 | 0.0391 | 0.0004 | 0.0336 | 0.0034 |
Golgi | 0.0228 | 0.0002 | 0.0014 | 0.006 | 0 | 0.0152 | 0.0084 | 0.0004 | 0.0052 | 0.0059 | 0.0068 | 0.0021 | 0.0097 | 0.0001 | 0.0112 | 0 | 0.0446 | 0.003 |
Lipid Particles | 0.0249 | 0.0006 | 0.0083 | 0.0237 | 0.0001 | 0.0056 | 0.0191 | 0.0006 | 0.0065 | 0.0109 | 0.0376 | 0.0028 | 0.0118 | 0.0003 | 0.0045 | 0.0001 | 0.0092 | 0.0051 |
Mitochondria | 0.0057 | 0.0003 | 0.0029 | 0.0113 | 0.0001 | 0.0219 | 0.0045 | 0.0002 | 0.0073 | 0.0014 | 0.034 | 0.0039 | 0.0128 | 0.0003 | 0.0253 | 0.0003 | 0.0231 | 0.0015 |
None | 0.6999 | 0.8825 | 0.8826 | 0.7685 | 0.9015 | 0.8076 | 0.7021 | 0.9143 | 0.8481 | 0.7679 | 0.8011 | 0.8814 | 0.7395 | 0.9201 | 0.7999 | 0.9769 | 0.7441 | 0.8862 |
Nuclear Periphery | 0.0022 | 0.0003 | 0.0011 | 0 | 0.0002 | 0.002 | 0.0081 | 0.0004 | 0.0004 | 0.0017 | 0.0009 | 0.0077 | 0.0015 | 0.0003 | 0.0013 | 0.0002 | 0.0003 | 0.0001 |
Nucleolus | 0.0006 | 0.0045 | 0.0009 | 0.0004 | 0 | 0.0003 | 0.0038 | 0.0007 | 0.0016 | 0.0048 | 0.0009 | 0.0005 | 0.0004 | 0.0003 | 0.0004 | 0.0003 | 0.0001 | 0.0002 |
Nucleus | 0.0006 | 0.0088 | 0.0017 | 0.0001 | 0.0001 | 0.0021 | 0.0062 | 0.0035 | 0.0044 | 0.0113 | 0.0019 | 0.0017 | 0.0011 | 0.0007 | 0.0021 | 0.0004 | 0.0002 | 0.0003 |
Peroxisomes | 0.0157 | 0.0003 | 0.008 | 0.0772 | 0 | 0.0066 | 0.0099 | 0.0003 | 0.0147 | 0.0077 | 0.0182 | 0.0047 | 0.0199 | 0.0001 | 0.0063 | 0.0003 | 0.0534 | 0.0058 |
Punctate Nuclear | 0.0087 | 0.012 | 0.0041 | 0.0005 | 0.0001 | 0.0052 | 0.009 | 0.0041 | 0.0055 | 0.0151 | 0.005 | 0.0039 | 0.0092 | 0.0023 | 0.0015 | 0.0017 | 0.0005 | 0.0022 |
Vacuole | 0.0044 | 0.0041 | 0.0016 | 0.0004 | 0.0001 | 0.0017 | 0.0035 | 0.0003 | 0.0016 | 0.002 | 0.002 | 0.0011 | 0.003 | 0.0005 | 0.0029 | 0.0003 | 0.0005 | 0.0001 |
Vacuole Periphery | 0.002 | 0.0002 | 0.0004 | 0.0002 | 0 | 0.0007 | 0.0012 | 0 | 0.0004 | 0.0002 | 0.001 | 0.0008 | 0.0018 | 0.0001 | 0.0009 | 0.0001 | 0.0004 | 0.0001 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 430.807 | 542.531 | 465.8085 | 398.5082 | 460.1139 | 437.027 | 550.0127 | 542.8704 | 574.3788 | 463.5739 |
Translational Efficiency | 1.1654 | 1.0766 | 0.9237 | 1.3694 | 0.967 | 1.0119 | 1.0947 | 0.9186 | 0.9186 | 0.9911 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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