Standard name
Description Subunit of Golgi mannosyltransferase complex; this complex mediates elongation of the polysaccharide mannan backbone; forms a separate complex with Van1p that is also involved in backbone elongation; this complex also contains Anp1p, Mnn10p, Mnn11p, and Hoc1p

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud 0 0 0.05 0 0.05 0.07 0.05 0.08 0 0.08 0.07 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.21 0.12 0.07 0.17 0.06 0.05 0 0.05 0 0 0 0.06 0.09 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.16 0.36 0.28 0.42 0.47 0.17 0.23 0.34 0.27 0.08 0.10 0.34 0.46 0.40 0.42 0.35 0.30 0.07 0.42 0
Golgi 0 0.11 0 0 0 0 0 0 0.05 0.06 0.08 0.31 0.17 0.30 0.29 0.34 0.40 0.42 0.06 1.00
Mitochondria 0.55 0.45 0.58 0.31 0.45 0.74 0.63 0.48 0.57 0.84 0.78 0.32 0.16 0.17 0.10 0.14 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.18 0.18 0.28 0.15 0.26 0.32 0.28 0.15 0.17 0.05 0.13 0.15 0 0.05 0.13 0.08 0.38 0
SpindlePole 0 0.05 0.08 0.08 0.11 0.07 0.11 0.16 0.12 0.07 0.07 0.14 0.17 0.15 0 0 0 0 0.14 0
Vac/Vac Membrane 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 3 4 0 1 7 0 0 8 6 10 7 6 3 2 15 10 0 0 0
Bud 0 7 24 3 25 61 52 70 35 54 60 10 11 8 0 1 1 0 0 0
Bud Neck 0 0 0 0 0 0 1 1 0 0 0 0 3 0 0 1 1 0 0 0
Bud Site 0 0 0 0 5 7 6 5 6 13 10 0 0 0
Cell Periphery 0 1 0 3 3 4 2 3 2 3 3 1 1 2 0 0 0 0 0 0
Cytoplasm 8 25 32 42 27 41 41 48 29 16 31 29 40 24 2 1 6 0 0 0
Endoplasmic Reticulum 1 2 1 2 0 6 2 2 2 1 11 11 8 10 0 1 3 0 0 0
Endosome 6 77 125 102 223 137 224 313 220 57 89 161 210 177 34 112 154 0 0 0
Golgi 1 23 7 11 4 8 10 18 43 45 73 146 77 133 24 109 208 0 0 0
Mitochondria 21 95 253 76 213 604 602 445 466 594 689 149 73 74 8 44 46 0 0 0
Nucleus 1 0 3 1 0 3 5 4 1 0 2 1 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 1 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 3 0 5 1 5 16 24 12 12 9 15 1 4 3 0 0 0 0 0 0
Peroxisomes 0 9 80 43 135 124 247 291 227 109 151 26 60 67 1 14 66 0 0 0
SpindlePole 1 10 33 19 53 56 108 152 95 47 61 68 79 65 2 4 6 0 0 0
Vac/Vac Membrane 3 11 5 1 7 7 17 18 18 8 12 12 15 12 2 8 2 0 0 0
Unique Cell Count 38 212 439 245 478 816 959 923 814 710 889 473 458 445 83 323 516 2 1 1
Labelled Cell Count 47 263 573 305 701 1081 1341 1382 1165 963 1217 622 588 579 83 323 516 2 1 1

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Golgi

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.6 5.7 6.1 5.7 5.2 5.2 5.8 5.8 5.6 5.8 6.9 6.8 7.1
Std Deviation (1e-4) 1.6 1.4 1.6 1.8 1.7 1.3 1.7 1.8 1.4 1.3 1.5 0.8 1.1 0.9
Intensity Change (Log2) 0.1 0.0 -0.1 -0.1 0.0 0.0 -0.0 0.0 0.3 0.3 0.3

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0.8 0 0
Bud -2.7 -0.2 1.4 0 1.4 -0.9 1.4 0.9 -2.7 -2.4 -2.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.4 -2.2 -3.7 -3.6 -3.9 -3.6 -4.9 -4.2 -1.3 0.1 -1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 2.8 0 2.7
Endosome 3.5 5.7 -4.9 -2.1 2.0 -0.5 -9.2 -8.6 1.8 5.4 3.5
Golgi 2.3 -1.1 -1.0 -0.9 0.5 3.2 3.8 4.8 11.8 7.8 11.5
Mitochondria -6.7 -4.0 5.9 1.8 -3.3 -0.1 9.7 7.5 -7.9 -13.0 -12.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.1 1.7 0.3 0.5 0.2 0.8 0 0 0
Peroxisomes -0.2 3.6 -1.4 3.1 5.2 3.8 -1.3 -0.6 -6.0 -2.1 -1.3
SpindlePole 0.1 1.9 -0.4 2.2 4.5 2.3 -0.6 -0.4 3.3 4.4 3.4
Vacuole 0 0 0 0 0 0 0 0 0.8 0 0