Standard name
Description TFIID subunit (47 kDa); involved in promoter binding and RNA polymerase II transcription initiation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.05 0.06 0.06 0.05 0 0 0 0.05 0 0.05 0.13 0.16 0.07 0.08 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0 0 0 0 0.1 0.31 0.18 0.33 0.21 0.47 0.26 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Nucleus 0.88 0.87 0.91 0.92 0.89 0.85 0.77 0.82 0.74 0.74 0.7 0.72 0.91 0.85 0.81 0.84 0.79 0.82 0.77 0.82 0.77 0.69 0.63 0.51
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.37 0.22 0.1 0.07 0.06 0.1 0.2 0.18 0.23 0.23 0.25 0.26 0.09 0 0.08 0.06 0.18 0.18 0.15 0.12 0.13 0.2 0.24 0.32
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 1 2 2 2 4 0 1 0 1 0 3 2 0 1 2 1 0 2 5
Bud Neck 0 0 0 0 1 0 1 0 3 4 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 1 0 0 1 4 7 7 27 16 6 11 0 0 0 0 1 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 8 8 12 18 17 20 8 11 10 10 1 8 31 41 23 11 16 0 0 0 1 0 3
Endoplasmic Reticulum 0 2 0 0 0 0 0 0 0 2 0 0 0 0 0 3 1 4 0 0 0 0 0 1
Endosome 0 0 0 0 1 3 0 2 0 1 0 1 1 1 2 0 2 3 1 3 0 0 1 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 1 0 2 0
Mitochondria 22 10 3 5 5 29 116 55 178 88 90 60 0 1 0 4 8 20 0 1 1 0 2 1
Nucleus 150 202 157 222 264 258 287 248 394 311 135 166 139 206 206 271 112 231 132 197 146 108 141 148
Nuclear Periphery 1 1 1 1 0 3 2 1 2 1 1 1 1 0 0 0 1 3 0 0 0 0 0 0
Nucleolus 64 50 17 18 18 31 74 54 124 96 48 61 14 9 20 20 26 51 25 28 24 30 53 91
Peroxisomes 0 0 0 0 0 1 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 5 2 5 3 3 6 10 7 0 7 1 4 2 4 2 1 3 0 3 2 4 10
Vac/Vac Membrane 0 2 2 1 1 3 1 3 2 2 0 5 0 1 0 9 4 9 1 3 5 6 14 15
Unique Cell Count 171 232 172 241 298 304 375 301 535 419 192 231 152 243 255 322 141 282 171 241 190 158 225 289
Labelled Cell Count 240 277 193 261 314 353 513 387 753 542 291 314 164 255 272 338 170 339 171 241 190 158 225 289


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 8.7 7.4 7.2 6.8 5.5 5.0 5.4 4.8 5.2 4.5 4.9 8.1 8.1 8.2 10.6 9.5 9.6 7.7 7.9 8.5
Std Deviation (1e-4) 1.0 1.9 1.5 1.9 1.4 1.4 1.5 1.2 1.1 1.3 1.5 1.0 1.7 1.9 2.0 2.4 2.7 2.5 1.5 1.6 1.8
Intensity Change (Log2) – – – -0.04 -0.13 -0.42 -0.55 -0.45 -0.63 -0.5 -0.7 -0.6 0.14 0.13 0.15 0.52 0.36 0.38 0.06 0.1 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.6 0.4 0.3 -1.2 0 -1.5 0.2 0 0.3 2.8 3.6 1.1 1.2 0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.3 7.7 5.3 0 5.9 0 0 0 0 0 0 0 2.5
Nucleus 0.3 -0.9 -2.0 -4.1 -2.7 -4.9 -4.6 -5.0 -4.8 0.1 -2.0 -3.0 -2.2 -3.0 -2.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.9 -1.5 0.1 2.9 2.4 0 3.7 0 0 -0.2 -2.6 -0.7 -1.5 2.2 2.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Taf3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available