Standard name | |
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Description | TFIID subunit (47 kDa); involved in promoter binding and RNA polymerase II transcription initiation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 4 | 0 | 1 | 0 | 1 | 0 | 3 | 2 | 0 | 1 | 2 | 1 | 0 | 2 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 1 | 0 | 0 | 1 | 4 | 7 | 7 | 27 | 16 | 6 | 11 | 0 | 0 | 0 | 0 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 8 | 8 | 12 | 18 | 17 | 20 | 8 | 11 | 10 | 10 | 1 | 8 | 31 | 41 | 23 | 11 | 16 | 0 | 0 | 0 | 1 | 0 | 3 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 1 | 2 | 0 | 2 | 3 | 1 | 3 | 0 | 0 | 1 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 0 |
Mitochondria | 22 | 10 | 3 | 5 | 5 | 29 | 116 | 55 | 178 | 88 | 90 | 60 | 0 | 1 | 0 | 4 | 8 | 20 | 0 | 1 | 1 | 0 | 2 | 1 |
Nucleus | 150 | 202 | 157 | 222 | 264 | 258 | 287 | 248 | 394 | 311 | 135 | 166 | 139 | 206 | 206 | 271 | 112 | 231 | 132 | 197 | 146 | 108 | 141 | 148 |
Nuclear Periphery | 1 | 1 | 1 | 1 | 0 | 3 | 2 | 1 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 64 | 50 | 17 | 18 | 18 | 31 | 74 | 54 | 124 | 96 | 48 | 61 | 14 | 9 | 20 | 20 | 26 | 51 | 25 | 28 | 24 | 30 | 53 | 91 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 5 | 2 | 5 | 3 | 3 | 6 | 10 | 7 | 0 | 7 | 1 | 4 | 2 | 4 | 2 | 1 | 3 | 0 | 3 | 2 | 4 | 10 |
Vac/Vac Membrane | 0 | 2 | 2 | 1 | 1 | 3 | 1 | 3 | 2 | 2 | 0 | 5 | 0 | 1 | 0 | 9 | 4 | 9 | 1 | 3 | 5 | 6 | 14 | 15 |
Unique Cell Count | 171 | 232 | 172 | 241 | 298 | 304 | 375 | 301 | 535 | 419 | 192 | 231 | 152 | 243 | 255 | 322 | 141 | 282 | 171 | 241 | 190 | 158 | 225 | 289 |
Labelled Cell Count | 240 | 277 | 193 | 261 | 314 | 353 | 513 | 387 | 753 | 542 | 291 | 314 | 164 | 255 | 272 | 338 | 170 | 339 | 171 | 241 | 190 | 158 | 225 | 289 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 6.3 | 8.7 | 7.4 | 7.2 | 6.8 | 5.5 | 5.0 | 5.4 | 4.8 | 5.2 | 4.5 | 4.9 | 8.1 | 8.1 | 8.2 | 10.6 | 9.5 | 9.6 | 7.7 | 7.9 | 8.5 |
Std Deviation (1e-4) | 1.0 | 1.9 | 1.5 | 1.9 | 1.4 | 1.4 | 1.5 | 1.2 | 1.1 | 1.3 | 1.5 | 1.0 | 1.7 | 1.9 | 2.0 | 2.4 | 2.7 | 2.5 | 1.5 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.13 | -0.42 | -0.55 | -0.45 | -0.63 | -0.5 | -0.7 | -0.6 | 0.14 | 0.13 | 0.15 | 0.52 | 0.36 | 0.38 | 0.06 | 0.1 | 0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | 0.6 | 0.4 | 0.3 | -1.2 | 0 | -1.5 | 0.2 | 0 | 0.3 | 2.8 | 3.6 | 1.1 | 1.2 | 0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.3 | 7.7 | 5.3 | 0 | 5.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 |
Nucleus | 0.3 | -0.9 | -2.0 | -4.1 | -2.7 | -4.9 | -4.6 | -5.0 | -4.8 | 0.1 | -2.0 | -3.0 | -2.2 | -3.0 | -2.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.9 | -1.5 | 0.1 | 2.9 | 2.4 | 0 | 3.7 | 0 | 0 | -0.2 | -2.6 | -0.7 | -1.5 | 2.2 | 2.4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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