Standard name
Description Vacuolar membrane protein; transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; cells lacking Ncr1p exhibit high levels of long chain bases (LCB), similar to the accumulation of high amounts of lipids observed in patients with Neimann-Pick C, a disease caused by loss-of-function mutations in NPC1, the functional ortholog of Ncr1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.19 0.13 0.44 0.5 0.5 0.43 0.4 0.38 0.41 0.46 0.44 0.22 0.2 0.18 0.5 0.45 0.35 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.05 0 0 0.05 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0.16 0.2 0.17 0.07 0.07 0.05 0 0 0 0 0 0 0.06 0.07 0.07 0.12 0 0 0.08 0.05 0.05 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0.16 0 0.2 0.27 0.25 0.39 0.44 0.45 0.38 0 0 0 0.09 0.09 0 0 0 0 0 0 0
Nucleus 0.1 0.05 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0.05 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.83 0.82 0.8 0.41 0.46 0.35 0.41 0.4 0.3 0.3 0.22 0.33 0.7 0.75 0.77 0.48 0.44 0.59 0.87 0.82 0.79 0.86 0.81 0.84
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 0 0 4 1 1 2 0 0 0 1 0 1 0 0 0 1 0 0 0
Bud 0 0 2 1 2 3 5 6 8 5 14 4 1 1 1 0 0 0 6 0 1 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 4 6 10 9 8 8 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 71 26 49 127 147 187 209 222 165 189 199 203 22 25 36 220 116 86 3 5 2 1 7 5
Endoplasmic Reticulum 6 2 2 4 8 19 7 20 23 20 21 13 1 2 5 16 9 13 2 0 4 0 4 0
Endosome 12 3 59 57 51 26 32 27 14 10 6 4 3 5 12 32 18 30 25 6 29 10 17 19
Golgi 2 1 4 1 1 4 1 7 2 0 2 2 0 0 0 2 0 3 1 2 2 0 0 1
Mitochondria 7 5 25 47 5 73 131 143 167 204 193 178 0 0 0 40 23 8 5 2 6 3 5 5
Nucleus 53 7 7 2 0 3 2 5 4 2 3 2 10 10 10 9 14 10 10 4 2 2 6 6
Nuclear Periphery 7 1 2 1 0 2 4 4 2 8 8 5 0 0 1 0 3 1 13 1 24 6 12 5
Nucleolus 0 0 2 1 0 1 0 3 2 2 0 1 0 1 0 0 2 1 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 3 1 0 1 0 0 0 1 2
Vac/Vac Membrane 448 114 290 119 136 131 199 223 129 138 94 151 71 91 156 213 113 143 498 126 303 173 271 293
Unique Cell Count 539 139 364 290 293 372 486 562 430 460 433 463 101 122 202 442 255 244 572 154 383 202 335 349
Labelled Cell Count 606 159 442 361 351 450 595 671 527 588 550 571 108 135 222 535 300 295 572 154 383 202 335 349


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 7.2 6.8 6.9 7.4 6.9 7.1 7.6 7.3 7.1 7.3 7.4 7.5 7.2 7.7 5.8 6.0 7.3 6.9 7.9 8.0
Std Deviation (1e-4) 1.3 1.5 1.5 2.1 1.5 1.7 1.6 1.5 1.6 1.6 1.6 1.6 1.2 1.3 1.2 1.5 1.6 2.2 1.4 2.0 1.9
Intensity Change (Log2) 0.01 0.13 0.03 0.07 0.16 0.1 0.06 0.11 0.13 0.14 0.08 0.18 -0.24 -0.19 0.11 0.03 0.21 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 2.7 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8.7 10.2 10.7 9.3 8.5 7.9 8.7 9.9 9.4 2.1 1.9 1.4 10.9 8.9 6.3
Endoplasmic Reticulum 0 0 3.7 0 2.9 3.9 3.4 3.6 2.4 0 0 0 2.9 0 3.7
Endosome 1.1 0.4 -3.9 -4.5 -5.8 -6.3 -7.2 -7.6 -8.3 -3.5 -3.4 -3.5 -4.0 -3.4 -1.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.8 -3.2 5.1 7.5 7.2 10.5 11.9 11.9 10.5 -2.7 -3.0 -3.8 1.1 1.0 -1.9
Nucleus 0 0 0 0 0 -1.2 0 0 0 0 0 2.0 0.1 2.4 1.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -10.1 -8.9 -12.2 -11.3 -12.0 -14.0 -14.2 -16.3 -13.5 -2.0 -1.2 -0.7 -9.2 -9.1 -5.6


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ncr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available