Standard name
Description Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; RNR2 has a paralog, RNR4, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.08 0 0 0 0 0.05 0.06 0.07 0.05 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.9 0.91 0.87 0.77 0.61 0.6 0.45 0.49 0.43 0.42 0.94 0.96 0.99 0.92 0.89 0.85 0.86 0.93 0.83 0.9 0.9 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.11 0.08 0 0.06 0.05 0 0 0 0.05 0 0.05 0 0.07 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0.19 0.18 0.35 0.49 0.41 0.55 0.55 0.63 0.63 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.08 0.06 0.06 0.05 0.09 0.07 0 0 0 0 0.06 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 1 2 12 0 2 6 4 2 2 1 0 0 0 0 0 0 0 0 1 1
Bud 0 1 0 0 0 1 6 5 6 0 7 5 0 0 0 0 0 0 1 0 1 2 3 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 1 10 1 4 5 18 16 18 22 10 7 3 8 8 9 7 1 0 0 0 0 0 0
Cytoplasm 185 137 117 158 238 196 278 180 130 152 90 103 220 300 353 148 98 82 178 133 114 143 297 227
Endoplasmic Reticulum 1 2 5 3 7 8 16 12 32 24 5 14 11 9 5 5 6 4 11 2 9 0 11 6
Endosome 0 0 0 0 3 2 2 2 0 0 2 0 1 1 1 0 0 1 0 1 0 1 0 3
Golgi 1 0 0 0 2 0 0 2 1 0 1 1 1 0 0 0 2 2 2 1 0 0 0 0
Mitochondria 9 2 1 33 49 88 226 124 160 172 133 154 2 0 0 2 4 0 4 1 5 2 2 1
Nucleus 0 0 0 0 1 1 2 0 1 0 2 0 0 2 1 0 0 0 0 1 0 1 2 0
Nuclear Periphery 0 0 0 0 0 2 0 0 1 1 0 1 0 0 0 0 0 0 1 0 0 0 1 1
Nucleolus 1 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 0 5 3 9 7 36 19 17 15 19 17 2 1 3 6 7 13 2 0 0 5 3 7
Unique Cell Count 195 140 130 174 274 254 458 300 289 312 211 246 233 311 358 160 110 96 208 144 137 160 331 257
Labelled Cell Count 202 144 139 198 314 312 596 360 368 394 274 304 242 322 371 170 124 103 208 144 137 160 331 257


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 10.6 9.3 8.3 8.6 7.6 7.6 7.6 7.4 7.5 7.4 7.1 10.2 10.8 10.5 14.4 14.6 13.0 10.4 10.9 11.2
Std Deviation (1e-4) 1.8 2.3 2.4 2.1 2.2 2.1 2.0 1.9 1.7 1.7 2.2 1.6 2.0 2.2 2.2 2.6 2.5 3.0 2.2 2.4 2.4
Intensity Change (Log2) -0.16 -0.11 -0.29 -0.29 -0.3 -0.33 -0.32 -0.33 -0.39 0.14 0.22 0.17 0.63 0.65 0.48 0.16 0.23 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 -2.7 -1.8 -0.9 -0.6 -0.2 -1.1 -2.2 0 -2.5 0 -0.7 -0.4 0
Cytoplasm 0.2 -0.9 -3.1 -6.3 -6.2 -8.7 -8.1 -8.7 -9.0 1.6 2.7 4.5 0.8 -0.2 -1.1
Endoplasmic Reticulum 0 0 0 0 0.1 2.4 1.5 0 0.8 0.4 0 0 0 0.6 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.0 4.9 7.4 10.0 8.5 10.6 10.7 11.4 11.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 1.6 1.0 0.9 0.4 1.8 1.2 0 0 0 0 0.9 2.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rnr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available