Standard name
Human Ortholog
Description Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.22 0.22 0.17 0 0 0.08 0 0 0.05 0 0 0.07 0.2 0.12 0.1 0.21 0.14 0.2 0.07 0.05 0.07 0 0.07 0.09
Bud 0 0 0.06 0 0.06 0.07 0.07 0.08 0.06 0.07 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.12 0 0 0.05 0.06 0.05 0.05 0.05 0.05 0.05 0.06 0.07 0.08 0.25 0.09 0.13 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0.09 0.18 0.07 0 0.05 0.06 0 0 0 0 0.05 0.06 0.11 0.06 0.08 0.06 0.05 0 0 0 0 0 0 0
Endosome 0.09 0.05 0.21 0.13 0.15 0.06 0.07 0.06 0.07 0.11 0.06 0.05 0.18 0.16 0.19 0.24 0.23 0.24 0.26 0.22 0.24 0.26 0.27 0.21
Golgi 0.62 0.66 0.41 0.34 0.13 0.05 0 0 0.05 0 0 0 0.54 0.59 0.4 0.5 0.42 0.44 0.55 0.63 0.6 0.48 0.45 0.52
Mitochondria 0.22 0.2 0.5 0.73 0.75 0.81 0.82 0.79 0.81 0.77 0.84 0.8 0.08 0 0 0.11 0.13 0.09 0 0 0 0.06 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.06 0 0 0 0 0 0 0 0 0 0 0.08 0.11 0.1 0.09 0.15 0.13 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 49 37 43 12 16 39 22 17 22 23 19 38 41 25 21 62 35 49 16 9 21 8 16 22
Bud 4 1 16 11 24 33 42 47 29 37 28 48 6 4 3 9 4 8 1 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 0 0 0 4 8 6 8 10 8 14 0 0 0 0 0 0
Cell Periphery 2 2 3 1 2 2 1 2 4 2 4 5 1 1 1 5 2 3 0 0 0 0 0 0
Cytoplasm 24 20 8 11 18 31 30 28 25 27 26 34 15 16 50 28 33 23 4 1 1 4 4 4
Endoplasmic Reticulum 21 31 19 3 19 31 16 19 18 23 24 34 23 12 17 17 13 9 3 3 3 7 8 11
Endosome 20 9 54 43 57 31 42 37 36 58 29 28 38 32 39 73 59 59 66 42 70 56 61 53
Golgi 140 113 105 108 51 23 15 19 23 12 7 4 113 119 81 152 105 107 142 123 176 105 100 135
Mitochondria 49 34 130 233 290 393 457 491 391 408 406 419 17 4 0 34 32 21 5 5 7 13 13 11
Nucleus 0 0 0 0 1 2 1 4 0 2 1 1 0 0 0 0 1 1 0 0 0 1 0 0
Nuclear Periphery 1 1 2 1 2 1 3 4 7 5 6 1 3 0 1 0 3 1 2 0 1 1 1 1
Nucleolus 1 2 6 6 4 10 14 55 16 9 15 12 2 0 1 3 4 2 0 0 0 0 0 0
Peroxisomes 0 0 1 5 6 3 9 14 6 9 3 9 1 1 0 0 1 0 2 0 5 4 6 4
SpindlePole 1 0 1 2 3 7 15 14 4 26 12 16 4 3 5 10 12 15 1 2 2 3 0 1
Vac/Vac Membrane 27 10 8 8 12 8 16 16 11 13 4 6 16 22 20 28 39 32 9 4 2 7 8 10
Unique Cell Count 225 172 259 320 389 483 560 620 482 531 481 526 209 201 201 301 253 245 259 196 293 219 225 259
Labelled Cell Count 339 260 396 444 505 618 691 773 600 664 592 669 280 239 239 421 343 330 259 196 293 219 225 259


Early Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 11.5 8.4 8.4 7.3 6.9 6.3 6.4 6.6 5.9 6.3 6.3 11.2 11.2 11.1 11.4 10.8 10.9 12.4 11.9 12.7
Std Deviation (1e-4) 1.6 1.7 1.1 1.3 1.1 1.3 1.6 1.4 1.3 1.3 1.4 1.3 1.7 1.4 1.4 2.0 1.9 1.9 2.4 2.1 2.0
Intensity Change (Log2) -0.01 -0.19 -0.28 -0.41 -0.39 -0.36 -0.51 -0.42 -0.41 0.42 0.41 0.41 0.44 0.36 0.38 0.56 0.5 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -5.2 -5.4 -3.5 -6.2 -7.4 -5.5 -5.9 -5.9 -4.1 0.8 -1.2 -1.9 1.2 -0.9 1.0
Bud -1.6 0 0.3 0.7 0.7 -0.1 0.4 -0.2 1.4 -1.7 -2.2 -2.5 -1.8 -2.7 -1.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 1.0 1.9 1.4 1.0 1.3 1.3 1.4 2.0 2.0 2.3 7.0 3.0 4.1 2.9
Endoplasmic Reticulum -4.0 -1.3 -0.5 -2.9 -2.8 -2.1 -1.8 -1.3 -0.5 1.4 -0.6 0.4 -0.8 -1.0 -1.8
Endosome -2.4 -2.1 -5.9 -5.5 -6.6 -5.3 -3.8 -6.1 -6.7 -0.7 -1.3 -0.4 1.0 0.7 0.9
Golgi -1.7 -8.0 -12.3 -14.2 -14.6 -12.3 -14.2 -14.2 -15.2 2.9 4.0 -0.1 2.4 0.2 0.7
Mitochondria 5.6 6.4 8.9 9.3 8.6 8.8 7.5 9.9 8.5 -9.7 -11.3 -11.9 -10.1 -9.1 -10.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.4 0 -0.2 0.2 3.5 0.8 0 0.6 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2.2 0 0 3.3 0 2.4 0 0 0 2.5 3.1 3.7
Vacuole -0.4 0 -1.3 -0.2 -0.4 -0.7 -0.5 0 0 2.2 3.4 3.1 3.0 4.8 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0358 5.9584 5.7141 4.8492 4.5652 5.3261 6.8494 8.4888 7.7341 10.7472 6.8988 7.6408 3.8976 5.8175 5.127 5.0597 4.2092 4.9737
Actin 0.0129 0.0236 0.0179 0.0848 0.0101 0.0231 0.0646 0.0244 0.0265 0.0283 0.0251 0.0138 0.0303 0.0113 0.0331 0.0044 0.0302 0.0172
Bud 0.0004 0.0006 0.0004 0.0005 0.0003 0.0002 0.0007 0.0026 0.0004 0.0002 0.0002 0.0002 0.0007 0.0003 0.0005 0.0002 0.0009 0.0002
Bud Neck 0.0011 0.0006 0.0037 0.0006 0.0004 0.0011 0.0044 0.0007 0.0006 0.0025 0.0004 0.0012 0.0009 0.0002 0.001 0.0005 0.0004 0.0007
Bud Periphery 0.0003 0.0003 0.0003 0.0004 0.0003 0.0002 0.0006 0.0018 0.0002 0.0001 0.0001 0.0001 0.0007 0.0002 0.0002 0.0001 0.0015 0.0001
Bud Site 0.0013 0.0031 0.0046 0.0007 0.0004 0.0003 0.0028 0.0091 0.0018 0.0005 0.0003 0.0007 0.0015 0.0006 0.0027 0.0003 0.002 0.0003
Cell Periphery 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0006 0.0001 0.0006 0.0022 0 0 0.0001 0 0 0 0.0002 0.0001
Cytoplasm 0.0138 0.031 0.0123 0.0075 0.0047 0.0095 0.0194 0.0308 0.0152 0.0063 0.006 0.0082 0.0064 0.0148 0.0066 0.0071 0.0491 0.0217
Cytoplasmic Foci 0.155 0.1926 0.1526 0.129 0.151 0.1517 0.1827 0.1796 0.1743 0.1102 0.1538 0.1523 0.164 0.1519 0.1536 0.1281 0.0953 0.125
Eisosomes 0.0001 0.0001 0.0001 0.0001 0 0 0.0004 0 0.0001 0 0 0 0.0001 0 0 0 0.0004 0
Endoplasmic Reticulum 0.0035 0.0016 0.0025 0.0007 0.0025 0.0012 0.0043 0.0009 0.0015 0.0006 0.0015 0.0007 0.0024 0.003 0.0015 0.0015 0.0056 0.0023
Endosome 0.4049 0.351 0.3764 0.3562 0.4041 0.3174 0.3486 0.3425 0.3367 0.2359 0.333 0.2472 0.3719 0.439 0.3722 0.548 0.4216 0.3513
Golgi 0.2959 0.3676 0.3727 0.3926 0.3959 0.4568 0.2978 0.3849 0.3868 0.443 0.4459 0.551 0.3573 0.3558 0.4023 0.2878 0.1697 0.4529
Lipid Particles 0.0069 0.0026 0.0126 0.0014 0.0041 0.0023 0.0108 0.0014 0.0054 0.004 0.0092 0.0035 0.0126 0.002 0.0031 0.0013 0.0601 0.003
Mitochondria 0.0086 0.0054 0.0068 0.005 0.0096 0.0207 0.0063 0.0051 0.0107 0.0037 0.0061 0.0052 0.0216 0.0105 0.0071 0.0024 0.0241 0.0082
None 0.0059 0.0003 0.0008 0.0001 0.0003 0.0001 0.0035 0.0003 0.0018 0.0001 0.0002 0.0001 0.0024 0.0001 0.0001 0.0001 0.0013 0.0001
Nuclear Periphery 0.0328 0.0009 0.0068 0.0002 0.0049 0.0002 0.0103 0.0001 0.0009 0.0002 0.0005 0.0001 0.0017 0.0004 0.0002 0.0003 0.0534 0.0002
Nucleolus 0.001 0.0001 0.0001 0 0.0001 0 0.0005 0 0.0004 0.0002 0 0.0001 0.0001 0 0 0 0.0006 0
Nucleus 0.0206 0.0009 0.0015 0.0001 0.0007 0.0001 0.0025 0.0001 0.0005 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.006 0.0001
Peroxisomes 0.0193 0.0132 0.0231 0.0167 0.0038 0.0098 0.0266 0.008 0.0162 0.0299 0.0149 0.0136 0.0207 0.0057 0.0131 0.0087 0.0605 0.0119
Punctate Nuclear 0.01 0.0003 0.0015 0.0001 0.0006 0.0001 0.0016 0.0002 0.0017 0.0003 0.0008 0.0002 0.001 0.0001 0.0002 0.0001 0.0021 0.0001
Vacuole 0.003 0.0025 0.0015 0.0017 0.0034 0.0026 0.0076 0.0058 0.0141 0.0944 0.0007 0.0007 0.0016 0.0021 0.0011 0.0041 0.006 0.0023
Vacuole Periphery 0.0025 0.0016 0.0017 0.0018 0.0027 0.0023 0.0034 0.0015 0.0038 0.0373 0.001 0.0009 0.0018 0.0019 0.0013 0.0049 0.009 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.7478 11.8028 21.2699 21.0971 17.1184 13.1958 20.0317 18.4103 15.316 24.6683
Translational Efficiency 2.0488 3.2217 1.4257 1.0707 1.9915 2.9666 1.933 2.0423 2.232 1.3866

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages endosome (13%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Aps3

Aps3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aps3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available