Standard name
Description Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.22 0.22 0.17 0 0 0.08 0 0 0.05 0 0 0.07 0.2 0.12 0.1 0.21 0.14 0.2 0.07 0.05 0.07 0 0.07 0.09
Bud 0 0 0.06 0 0.06 0.07 0.07 0.08 0.06 0.07 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.12 0 0 0.05 0.06 0.05 0.05 0.05 0.05 0.05 0.06 0.07 0.08 0.25 0.09 0.13 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0.09 0.18 0.07 0 0.05 0.06 0 0 0 0 0.05 0.06 0.11 0.06 0.08 0.06 0.05 0 0 0 0 0 0 0
Endosome 0.09 0.05 0.21 0.13 0.15 0.06 0.07 0.06 0.07 0.11 0.06 0.05 0.18 0.16 0.19 0.24 0.23 0.24 0.26 0.22 0.24 0.26 0.27 0.21
Golgi 0.62 0.66 0.41 0.34 0.13 0.05 0 0 0.05 0 0 0 0.54 0.59 0.4 0.5 0.42 0.44 0.55 0.63 0.6 0.48 0.45 0.52
Mitochondria 0.22 0.2 0.5 0.73 0.75 0.81 0.82 0.79 0.81 0.77 0.84 0.8 0.08 0 0 0.11 0.13 0.09 0 0 0 0.06 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.06 0 0 0 0 0 0 0 0 0 0 0.08 0.11 0.1 0.09 0.15 0.13 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 49 37 43 12 16 39 22 17 22 23 19 38 41 25 21 62 35 49 16 9 21 8 16 22
Bud 4 1 16 11 24 33 42 47 29 37 28 48 6 4 3 9 4 8 1 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 0 0 0 4 8 6 8 10 8 14 0 0 0 0 0 0
Cell Periphery 2 2 3 1 2 2 1 2 4 2 4 5 1 1 1 5 2 3 0 0 0 0 0 0
Cytoplasm 24 20 8 11 18 31 30 28 25 27 26 34 15 16 50 28 33 23 4 1 1 4 4 4
Endoplasmic Reticulum 21 31 19 3 19 31 16 19 18 23 24 34 23 12 17 17 13 9 3 3 3 7 8 11
Endosome 20 9 54 43 57 31 42 37 36 58 29 28 38 32 39 73 59 59 66 42 70 56 61 53
Golgi 140 113 105 108 51 23 15 19 23 12 7 4 113 119 81 152 105 107 142 123 176 105 100 135
Mitochondria 49 34 130 233 290 393 457 491 391 408 406 419 17 4 0 34 32 21 5 5 7 13 13 11
Nucleus 0 0 0 0 1 2 1 4 0 2 1 1 0 0 0 0 1 1 0 0 0 1 0 0
Nuclear Periphery 1 1 2 1 2 1 3 4 7 5 6 1 3 0 1 0 3 1 2 0 1 1 1 1
Nucleolus 1 2 6 6 4 10 14 55 16 9 15 12 2 0 1 3 4 2 0 0 0 0 0 0
Peroxisomes 0 0 1 5 6 3 9 14 6 9 3 9 1 1 0 0 1 0 2 0 5 4 6 4
SpindlePole 1 0 1 2 3 7 15 14 4 26 12 16 4 3 5 10 12 15 1 2 2 3 0 1
Vac/Vac Membrane 27 10 8 8 12 8 16 16 11 13 4 6 16 22 20 28 39 32 9 4 2 7 8 10
Unique Cell Count 225 172 259 320 389 483 560 620 482 531 481 526 209 201 201 301 253 245 259 196 293 219 225 259
Labelled Cell Count 339 260 396 444 505 618 691 773 600 664 592 669 280 239 239 421 343 330 259 196 293 219 225 259


Early Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 11.5 8.4 8.4 7.3 6.9 6.3 6.4 6.6 5.9 6.3 6.3 11.2 11.2 11.1 11.4 10.8 10.9 12.4 11.9 12.7
Std Deviation (1e-4) 1.6 1.7 1.1 1.3 1.1 1.3 1.6 1.4 1.3 1.3 1.4 1.3 1.7 1.4 1.4 2.0 1.9 1.9 2.4 2.1 2.0
Intensity Change (Log2) -0.01 -0.19 -0.28 -0.41 -0.39 -0.36 -0.51 -0.42 -0.41 0.42 0.41 0.41 0.44 0.36 0.38 0.56 0.5 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -5.2 -5.4 -3.5 -6.2 -7.4 -5.5 -5.9 -5.9 -4.1 0.8 -1.2 -1.9 1.2 -0.9 1.0
Bud -1.6 0 0.3 0.7 0.7 -0.1 0.4 -0.2 1.4 -1.7 -2.2 -2.5 -1.8 -2.7 -1.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 1.0 1.9 1.4 1.0 1.3 1.3 1.4 2.0 2.0 2.3 7.0 3.0 4.1 2.9
Endoplasmic Reticulum -4.0 -1.3 -0.5 -2.9 -2.8 -2.1 -1.8 -1.3 -0.5 1.4 -0.6 0.4 -0.8 -1.0 -1.8
Endosome -2.4 -2.1 -5.9 -5.5 -6.6 -5.3 -3.8 -6.1 -6.7 -0.7 -1.3 -0.4 1.0 0.7 0.9
Golgi -1.7 -8.0 -12.3 -14.2 -14.6 -12.3 -14.2 -14.2 -15.2 2.9 4.0 -0.1 2.4 0.2 0.7
Mitochondria 5.6 6.4 8.9 9.3 8.6 8.8 7.5 9.9 8.5 -9.7 -11.3 -11.9 -10.1 -9.1 -10.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.4 0 -0.2 0.2 3.5 0.8 0 0.6 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2.2 0 0 3.3 0 2.4 0 0 0 2.5 3.1 3.7
Vacuole -0.4 0 -1.3 -0.2 -0.4 -0.7 -0.5 0 0 2.2 3.4 3.1 3.0 4.8 4.1


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aps3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available