Standard name
Human Ortholog
Description Chorismate mutase; catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.99 0.98 0.95 0.85 0.88 0.65 0.79 0.85 0.68 0.74 0.99 1.0 1.0 0.94 0.94 0.79 0.97 0.96 0.96 0.93 0.94 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0 0.05 0.06 0 0.06 0 0 0 0 0.08 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.09 0 0.1 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.13 0.05 0.32 0.08 0.06 0.21 0.12 0 0 0 0 0 0.17 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 1 2 0 0 5 9 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 0 0 1 0 0 0 0 0 2 1 0 0 0 0 0 0 0 1 0 1 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 6 0 2 4 7 5 8 5 5 13 27 0 0 1 2 1 5 0 0 0 0 0 0
Cytoplasm 334 571 220 258 208 315 272 327 242 230 229 309 68 227 143 61 80 112 325 564 210 188 177 165
Endoplasmic Reticulum 0 2 0 4 5 7 17 7 15 16 10 26 2 0 0 1 7 4 1 2 1 2 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 2 0 0 1 2
Golgi 0 0 0 0 0 1 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0
Mitochondria 1 1 0 0 3 1 0 8 27 4 34 21 0 0 0 1 2 3 0 3 0 1 1 0
Nucleus 3 2 2 2 0 3 8 7 8 9 5 8 0 1 2 2 1 1 1 3 1 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 1 3 1 2 7 50 16 161 26 16 71 49 0 0 0 0 1 24 3 4 0 2 2 0
Unique Cell Count 337 580 222 264 220 371 310 500 307 270 338 417 69 227 143 65 85 141 337 586 220 203 188 176
Labelled Cell Count 344 586 223 268 228 384 319 521 323 280 373 451 70 228 146 67 92 151 337 586 220 203 188 176


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.4 15.7 17.6 17.5 13.0 14.5 13.7 13.7 11.5 12.2 11.9 11.9 18.2 17.8 18.0 17.6 18.8 18.7 18.5 19.6 19.4
Std Deviation (1e-4) 3.9 2.8 2.9 2.8 2.7 2.8 2.4 2.5 2.0 2.1 1.9 2.1 3.5 3.0 2.7 3.9 4.3 5.9 4.3 4.6 4.4
Intensity Change (Log2) – – – -0.0 -0.43 -0.27 -0.36 -0.36 -0.61 -0.53 -0.56 -0.56 0.05 0.02 0.03 0.01 0.09 0.09 0.08 0.15 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 3.0 3.9 0 0 0 0 0 0
Cytoplasm -1.2 -2.7 -5.6 -4.9 -9.8 -6.9 -5.5 -9.1 -8.0 -0.4 1.4 1.1 -2.6 -2.6 -6.5
Endoplasmic Reticulum 0 0 0 3.5 0 3.3 3.7 0 3.8 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.5 0 4.9 3.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 5.5 3.0 9.4 4.1 3.3 7.1 5.1 0 0 0 0 0 6.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.3268 13.9933 12.9616 11.8739 12.0622 12.5515 18.08 21.776 20.633 18.9662 17.5803 20.3013 14.2263 17.6254 15.4941 15.4057 15.3967 16.3191
Actin 0.0005 0.0003 0.0001 0.01 0.0001 0.0001 0.0195 0.0003 0.0046 0.0268 0.0091 0.0024 0.0032 0.0001 0.0039 0.0357 0.0054 0.0002
Bud 0.0006 0.0001 0.0001 0.0005 0.0001 0.0001 0.0012 0.0002 0.0002 0.0005 0.0003 0.0001 0.0006 0 0.0002 0.0001 0.0003 0.0001
Bud Neck 0.0003 0.0002 0.0004 0.0006 0.0005 0.0008 0.0018 0.0001 0.0004 0.0009 0.0006 0.0007 0.0013 0.0001 0.0004 0.0005 0.0009 0.0009
Bud Periphery 0.0002 0 0 0.0007 0 0 0.0012 0.0001 0.0002 0.0005 0.0004 0 0.0004 0 0.0001 0.0001 0.0002 0
Bud Site 0.0002 0.0001 0.0001 0.0004 0 0 0.0018 0.0002 0.001 0.0011 0.0002 0 0.0006 0.0001 0.0004 0.0002 0.0027 0
Cell Periphery 0.0001 0 0 0.0001 0 0 0.0002 0 0.0003 0.0001 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.9496 0.9799 0.9696 0.9386 0.9561 0.9526 0.8783 0.9902 0.9579 0.8717 0.8593 0.9629 0.9181 0.9919 0.9251 0.9353 0.8261 0.9757
Cytoplasmic Foci 0.0013 0.0007 0.0013 0.0024 0.0007 0.0015 0.0137 0.0007 0.0024 0.009 0.0258 0.0027 0.0202 0.0005 0.0087 0.0014 0.049 0.0017
Eisosomes 0 0 0 0.0001 0 0 0.0003 0 0.0001 0.0001 0.0001 0 0 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0037 0.0008 0.0006 0.0015 0.0012 0.0019 0.0045 0.0003 0.004 0.0008 0.0016 0.0015 0.0028 0.0004 0.0025 0.0008 0.001 0.0019
Endosome 0.0021 0.0004 0.0003 0.0022 0.0003 0.0012 0.0157 0.0002 0.0067 0.031 0.0183 0.002 0.0108 0.0002 0.0144 0.0009 0.0212 0.001
Golgi 0.0001 0 0 0.0009 0 0 0.0029 0 0.0011 0.0049 0.0106 0.001 0.0034 0 0.0036 0.0005 0.027 0
Lipid Particles 0.0001 0 0 0.0001 0 0 0.0026 0 0.001 0.0218 0.0239 0.0001 0.0022 0 0.0061 0.0001 0.0192 0
Mitochondria 0.0002 0.0001 0.0001 0.0069 0.0001 0.0001 0.0058 0.0001 0.0011 0.0015 0.0189 0.0008 0.007 0 0.0086 0.0001 0.0027 0.0001
None 0.0023 0.0006 0.001 0.0012 0.0006 0.0008 0.0036 0.0007 0.0013 0.0006 0.0033 0.0016 0.0036 0.0006 0.0011 0.0006 0.0006 0.0009
Nuclear Periphery 0.0073 0.0019 0.0016 0.0064 0.0043 0.0037 0.0144 0.0008 0.0021 0.002 0.0009 0.0021 0.0055 0.0006 0.0025 0.002 0.0009 0.0014
Nucleolus 0.0001 0 0 0 0 0 0.0008 0 0.0001 0.0002 0.0001 0 0.0001 0 0.0001 0 0.0001 0
Nucleus 0.0283 0.0139 0.0235 0.0221 0.0346 0.0353 0.0231 0.0057 0.0129 0.0145 0.0175 0.0206 0.013 0.0051 0.0117 0.0203 0.0043 0.0151
Peroxisomes 0 0 0 0.0032 0 0 0.0027 0 0.0003 0.0063 0.0075 0.0003 0.0032 0 0.0081 0.0003 0.0363 0
Punctate Nuclear 0.0007 0.0002 0.0006 0.0005 0.0004 0.0005 0.0031 0.0002 0.0008 0.0009 0.0006 0.0005 0.0017 0.0001 0.0011 0.0004 0.0013 0.0006
Vacuole 0.0021 0.0005 0.0004 0.0014 0.0008 0.0009 0.0019 0.0002 0.0014 0.0041 0.0006 0.0003 0.0015 0.0002 0.001 0.0005 0.0004 0.0003
Vacuole Periphery 0.0003 0.0001 0.0001 0.0002 0.0001 0.0002 0.0011 0 0.0003 0.0007 0.0005 0.0001 0.0008 0 0.0002 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 70.1256 57.5706 52.2132 52.4585 51.027 75.8081 38.483 38.851 43.7499 57.9016
Translational Efficiency 1.6215 1.1126 1.2415 1.4037 1.4866 1.3261 1.4756 1.1496 1.4216 1.424

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘