Standard name
Description Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0.09 0.13 0.18 0.08 0.09 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.14 0.5 0.37 0.14 0.35 0.34 0.31 0.53 0.5 0.51 0 0 0.17 0.05 0 0.05 0 0 0 0 0 0
Nucleus 0.95 0.9 0.92 0.87 0.86 0.79 0.78 0.75 0.71 0.67 0.66 0.89 0.83 0.7 0.86 0.84 0.81 0.87 0.88 0.87 0.87 0.68 0.44
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0.05 0.05 0.08 0.09 0.12 0.13 0.2 0.18 0.28 0.29 0 0 0.07 0 0 0 0.05 0 0 0 0.07 0.15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.07 0.05 0.12 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3
Bud 0 0 0 0 2 1 0 3 1 4 5 1 0 0 0 0 0 1 0 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 1 3 7 14 9 12 34 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 5 1 13 6 7 11 17 10 11 5 20 54 78 38 29 24 0 0 0 2 2 2
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 0 1 2 10 5 8 0 0 0 0 2 3
Endosome 0 0 0 1 4 1 1 2 0 0 0 0 2 2 5 4 2 1 1 0 1 4 28
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 3 0 0 0 2 2
Mitochondria 16 18 63 115 59 134 179 273 211 283 341 9 15 75 27 9 17 2 0 1 2 1 10
Nucleus 164 118 117 271 377 302 409 667 285 378 446 205 339 306 432 278 274 156 117 111 154 162 168
Nuclear Periphery 0 0 0 1 10 3 16 21 10 15 12 0 3 6 1 1 6 1 0 0 0 0 6
Nucleolus 14 7 6 24 39 45 70 178 74 157 195 3 14 30 10 3 11 9 4 4 4 17 57
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 4 4 2 8 2 4 1 0 0 1 2 3 6 0 1 0 0 5 18
Vac/Vac Membrane 1 0 0 0 3 1 2 6 1 3 3 2 1 12 18 13 22 3 5 9 9 29 71
Unique Cell Count 173 131 127 312 437 381 523 886 400 561 671 231 407 440 504 330 338 180 134 128 178 239 388
Labelled Cell Count 199 148 188 428 505 501 697 1191 604 867 1042 240 429 512 543 348 370 180 134 128 178 239 388


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 5.7 4.2 4.8 5.0 4.5 4.3 4.5 4.0 4.2 3.8 5.9 5.9 5.0 6.8 7.6 7.9 5.6 5.9 6.1
Std Deviation (1e-4) 0.9 1.0 0.5 1.3 1.0 0.9 1.0 1.1 1.0 1.0 0.8 1.3 1.2 1.3 1.2 1.6 1.5 1.2 1.5 1.2
Intensity Change (Log2) 0.19 0.25 0.11 0.04 0.1 -0.07 -0.01 -0.12 0.5 0.5 0.27 0.71 0.85 0.92 0.43 0.49 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 3.0 4.0 4.9 2.8 3.1 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -8.7 0 0 0 0 0 0 -10.3 -12.8 -7.5 -12.7 -12.3 -11.3
Nucleus -1.6 -1.8 -3.3 -3.6 -4.2 -4.8 -5.6 -5.8 -1.0 -2.5 -5.1 -1.9 -2.2 -2.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1.5 0 0 0 0 0 0 0 0 0.9 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 2.9


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fhl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available