Standard name
Description Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with LSB1 cooperatively inhibits the nucleation of actin filaments; short-lived protein whose levels increase in response to thermal stress; induces the formation of the [PIN+] and [RNQ+] prions when overproduced; PIN3 has a paralog, LSB1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.97 0.9 0.83 0.83 0.78 0.67 0.75 0.7 0.72 0.68 0.98 0.97 0.97 0.84 0.9 0.92 0.9 0.91 0.94 0.94 0.91 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0 0.05 0 0.09 0.1 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.08 0.25 0.07 0.27 0.31 0.21 0.21 0.17 0.19 0 0 0.05 0.18 0.1 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 1 3 0 0 2 2
Bud 0 0 0 0 0 3 0 1 1 1 2 0 0 0 0 0 1 0 0 0 3 0 2 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 5 2 3 1 3 8 2 7 8 7 1 1 2 4 4 4 0 0 0 0 0 0
Cytoplasm 161 330 188 185 269 138 209 244 174 203 149 140 315 407 422 178 179 175 161 310 182 308 341 378
Endoplasmic Reticulum 1 6 2 1 3 1 2 1 1 4 4 7 6 0 1 5 2 5 1 2 0 1 6 8
Endosome 0 0 1 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 3 3 1 0 3 14
Golgi 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 2 1 2 1 0 1 2 2
Mitochondria 3 1 0 3 1 18 6 17 10 27 21 26 1 0 0 7 3 2 1 3 0 0 1 0
Nucleus 0 2 1 1 1 4 4 6 3 13 6 9 4 1 0 4 5 3 0 3 2 2 2 4
Nuclear Periphery 0 1 0 0 0 0 0 0 0 2 1 2 0 0 0 0 0 0 1 0 0 0 0 4
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 3 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 4
Vac/Vac Membrane 4 1 5 17 81 12 73 112 50 61 35 40 3 18 20 38 19 17 2 4 0 4 3 4
Unique Cell Count 169 338 194 205 323 166 267 366 233 290 208 206 322 418 437 211 200 190 179 340 194 327 373 441
Labelled Cell Count 170 342 202 209 358 178 297 391 242 319 227 233 330 427 445 241 215 207 179 340 194 327 373 441


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 9.8 9.9 9.5 11.1 7.1 9.9 9.7 9.0 9.0 9.0 8.6 9.3 10.6 10.9 16.8 16.2 15.2 8.8 10.1 11.1
Std Deviation (1e-4) 1.2 1.8 1.7 1.5 1.7 1.7 2.2 1.9 2.3 2.1 2.4 2.0 1.9 2.0 1.9 6.1 5.2 5.8 1.8 2.0 2.3
Intensity Change (Log2) -0.07 0.16 -0.49 -0.01 -0.04 -0.15 -0.15 -0.15 -0.2 -0.09 0.1 0.14 0.75 0.71 0.61 -0.17 0.02 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.7 0.5 0 0 0 0 0 0
Cytoplasm -2.7 -4.7 -4.5 -5.7 -8.1 -6.4 -7.4 -6.9 -7.5 0.6 0.3 -0.2 -4.3 -2.9 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.7 0 3.0 0 4.4 4.5 5.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 2.6 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.5 6.7 2.1 7.0 7.8 5.8 5.8 4.8 5.3 0 1.0 1.2 5.0 2.9 2.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pin3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available