Standard name
Description Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability; relocalizes to the cytosol in response to hypoxia

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0.05 0 0.05 0 0 0 0 0.05 0 0.06 0.08 0.1 0 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0 0 0 0.12 0.06 0.18 0.13 0.19 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.91 0.98 0.94 0.97 0.91 0.89 0.88 0.85 0.87 0.85 0.83 0.93 0.91 0.88 0.89 0.89 0.93 0.93 0.89 0.94 0.8 0.76 0.71
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.07 0.09 0.07 0.14 0.17 0.21 0.16 0 0 0 0 0 0 0 0 0 0.12 0.09 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0.1 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 1 1 3 0 0 0 0 0 0 0 3 0 0 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 0 5 3 5 7 3 5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 5 4 1 7 6 12 13 8 9 6 9 6 12 18 20 10 9 4 0 2 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 1 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 3 0 0 0 1 1
Mitochondria 11 6 0 1 0 6 39 14 55 38 32 21 1 0 0 1 0 3 3 2 1 0 2 4
Nucleus 310 78 106 134 216 201 291 211 260 253 141 175 177 210 177 217 161 210 305 91 111 147 206 199
Nuclear Periphery 2 0 0 1 4 0 1 3 6 1 6 2 2 0 2 2 0 2 0 0 0 0 0 2
Nucleolus 3 1 2 2 0 15 28 17 42 49 35 34 1 2 1 4 6 3 0 0 0 21 25 28
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 0 1 0 0 0 0 2 0
Vac/Vac Membrane 2 1 2 0 1 1 0 3 1 0 2 1 1 3 2 12 4 8 6 3 2 8 26 41
Unique Cell Count 327 86 108 142 222 221 328 241 305 292 166 212 191 230 201 243 181 225 328 102 119 183 272 283
Labelled Cell Count 335 90 111 145 228 235 377 260 380 355 232 244 197 234 203 251 181 233 328 102 119 183 272 283


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 9.2 7.5 8.0 8.6 7.0 6.5 7.4 6.3 6.2 5.8 6.4 9.3 9.5 9.7 11.4 12.5 11.9 8.5 8.7 9.6
Std Deviation (1e-4) 1.1 2.2 1.4 1.4 2.2 1.5 1.7 2.2 2.6 1.5 1.5 1.7 2.0 2.1 2.4 3.2 3.2 3.0 1.8 1.7 1.8
Intensity Change (Log2) 0.1 0.21 -0.09 -0.19 -0.01 -0.24 -0.28 -0.36 -0.23 0.31 0.35 0.38 0.61 0.75 0.68 0.19 0.22 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 2.6 3.0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.8 0 4.7 3.9 4.9 3.4 0 0 0 0 0 0
Nucleus -1.5 -0.5 -2.5 -3.0 -3.2 -3.6 -3.4 -3.6 -4.0 -2.0 -2.4 -3.0 -2.8 -2.9 -1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.9 2.4 2.0 3.5 4.0 4.6 3.8 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aos1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available