Standard name
Description Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0.07 0 0 0 0 0 0 0 0.1 0.14 0.21 0.07 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0.07 0.23 0.21 0.54 0.65 0.44 0.54 0.74 0.74 0.71 0 0 0 0 0 0 0 0 0 0 0
Nucleus 1.0 0.93 0.9 0.88 0.85 0.79 0.73 0.79 0.74 0.71 0.71 0.74 0.89 0.85 0.77 0.88 0.78 0.87 0.91 0.84 0.79 0.64 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.08 0 0.08 0.1 0.21 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
Bud 0 0 2 1 2 6 5 8 8 9 9 10 0 0 0 0 0 0 1 6 5 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 6 11 12 13 19 21 20 27 0 0 0 0 0
Cell Periphery 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 9 21 12 26 23 26 27 23 26 16 21 32 57 79 5 4 0 0 3 9 11 7
Endoplasmic Reticulum 0 1 2 0 0 1 0 1 0 0 0 0 1 0 1 0 1 0 0 2 0 2 2
Endosome 0 0 4 1 0 0 0 0 0 0 0 0 1 0 3 0 0 0 1 4 3 4 5
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 1 2 0 0 0
Mitochondria 3 4 36 68 79 298 429 294 300 495 436 512 1 1 0 3 1 0 0 5 3 13 12
Nucleus 37 184 440 262 313 434 481 525 408 471 418 532 275 353 296 65 29 53 175 425 282 283 208
Nuclear Periphery 0 0 0 1 2 7 4 9 3 10 6 14 0 0 1 2 0 0 0 1 0 2 4
Nucleolus 1 1 2 4 2 1 7 19 9 10 15 27 0 1 2 0 0 0 0 2 5 13 11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 2 0 0 3 2 1 0 1 2 1 6 3 0 0 1 1 5 5 4 6
Vac/Vac Membrane 0 4 8 5 11 11 12 7 6 18 10 15 2 3 11 2 5 4 8 38 34 94 87
Unique Cell Count 37 198 488 299 370 551 659 668 551 666 591 721 308 413 384 74 37 62 193 507 357 445 360
Labelled Cell Count 41 205 516 357 441 792 979 906 777 1060 931 1160 315 422 396 78 40 62 193 507 357 445 360


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 6.1 4.8 4.4 4.6 3.8 3.6 3.8 3.6 3.3 3.1 3.2 6.1 6.2 6.0 7.3 6.5 6.0 6.3 6.7
Std Deviation (1e-4) 0.8 1.1 1.0 1.1 2.1 1.2 1.1 1.1 1.0 0.7 0.9 0.7 1.0 1.2 1.4 1.9 1.3 1.0 1.3 1.3
Intensity Change (Log2) -0.14 -0.06 -0.35 -0.42 -0.36 -0.43 -0.53 -0.61 -0.58 0.35 0.36 0.32 0.6 0.43 0.33 0.39 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 1.7 -0.1 -0.3 -0.2 -0.1 -0.3 -1.4 -1.3 3.4 5.1 7.5 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -4.6 -5.4 -5.4 -1.0 0
Nucleus -1.1 -2.5 -5.0 -7.2 -5.2 -6.7 -8.0 -7.9 -7.0 -0.4 -2.2 -5.3 -0.6 -2.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.3 0.4 0.2 -0.9 -0.8 1.2 0.1 0.6 0 -1.2 1.2 0 0