Standard name | |
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Description | Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Bud | 0 | 0 | 2 | 1 | 2 | 6 | 5 | 8 | 8 | 9 | 9 | 10 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 6 | 5 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 6 | 11 | 12 | 13 | 19 | 21 | 20 | 27 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 9 | 21 | 12 | 26 | 23 | 26 | 27 | 23 | 26 | 16 | 21 | 32 | 57 | 79 | – | 5 | 4 | 0 | 0 | 3 | 9 | 11 | 7 |
Endoplasmic Reticulum | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 2 |
Endosome | 0 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | – | 0 | 0 | 0 | 1 | 4 | 3 | 4 | 5 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 1 | 1 | 2 | 0 | 0 | 0 |
Mitochondria | 3 | 4 | 36 | 68 | 79 | 298 | 429 | 294 | 300 | 495 | 436 | 512 | 1 | 1 | 0 | – | 3 | 1 | 0 | 0 | 5 | 3 | 13 | 12 |
Nucleus | 37 | 184 | 440 | 262 | 313 | 434 | 481 | 525 | 408 | 471 | 418 | 532 | 275 | 353 | 296 | – | 65 | 29 | 53 | 175 | 425 | 282 | 283 | 208 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 2 | 7 | 4 | 9 | 3 | 10 | 6 | 14 | 0 | 0 | 1 | – | 2 | 0 | 0 | 0 | 1 | 0 | 2 | 4 |
Nucleolus | 1 | 1 | 2 | 4 | 2 | 1 | 7 | 19 | 9 | 10 | 15 | 27 | 0 | 1 | 2 | – | 0 | 0 | 0 | 0 | 2 | 5 | 13 | 11 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 2 | 0 | 0 | 3 | 2 | 1 | 0 | 1 | 2 | 1 | 6 | 3 | – | 0 | 0 | 1 | 1 | 5 | 5 | 4 | 6 |
Vac/Vac Membrane | 0 | 4 | 8 | 5 | 11 | 11 | 12 | 7 | 6 | 18 | 10 | 15 | 2 | 3 | 11 | – | 2 | 5 | 4 | 8 | 38 | 34 | 94 | 87 |
Unique Cell Count | 37 | 198 | 488 | 299 | 370 | 551 | 659 | 668 | 551 | 666 | 591 | 721 | 308 | 413 | 384 | 74 | 37 | 62 | 193 | 507 | 357 | 445 | 360 | |
Labelled Cell Count | 41 | 205 | 516 | 357 | 441 | 792 | 979 | 906 | 777 | 1060 | 931 | 1160 | 315 | 422 | 396 | 78 | 40 | 62 | 193 | 507 | 357 | 445 | 360 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 6.1 | 4.8 | 4.4 | 4.6 | 3.8 | 3.6 | 3.8 | 3.6 | 3.3 | 3.1 | 3.2 | 6.1 | 6.2 | 6.0 | – | 7.3 | 6.5 | 6.0 | 6.3 | 6.7 |
Std Deviation (1e-4) | 0.8 | 1.1 | 1.0 | 1.1 | 2.1 | 1.2 | 1.1 | 1.1 | 1.0 | 0.7 | 0.9 | 0.7 | 1.0 | 1.2 | 1.4 | – | 1.9 | 1.3 | 1.0 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.06 | -0.35 | -0.42 | -0.36 | -0.43 | -0.53 | -0.61 | -0.58 | 0.35 | 0.36 | 0.32 | – | 0.6 | 0.43 | 0.33 | 0.39 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cytoplasm | -0.2 | 1.7 | -0.1 | -0.3 | -0.2 | -0.1 | -0.3 | -1.4 | -1.3 | 3.4 | 5.1 | 7.5 | – | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.6 | -5.4 | -5.4 | – | -1.0 | 0 |
Nucleus | -1.1 | -2.5 | -5.0 | -7.2 | -5.2 | -6.7 | -8.0 | -7.9 | -7.0 | -0.4 | -2.2 | -5.3 | – | -0.6 | -2.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | 0 | 1.3 | 0.4 | 0.2 | -0.9 | -0.8 | 1.2 | 0.1 | 0.6 | 0 | -1.2 | 1.2 | – | 0 | 0 |