Standard name
Description RNA polymerase subunit ABC23; common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.54 0.46 0.23 0.25 0.31 0.33 0.25 0.33 0.33 0.35 0.31 0.35 0.53 0.38 0.43 0.44 0.51 0.47 0.11 0.08 0.10 0.16 0.12 0.10
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.13 0.23 0.25 0 0.29 0.18 0.13 0.20 0.20 0.19 0 0 0.05 0.14 0.07 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.23 0.12 0.38 0.36 0.22 0.15 0.19 0.24 0.25 0.20 0.20 0.20 0.23 0.16 0.15 0.12 0 0.10 0.64 0.65 0.73 0.39 0.41 0.40
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.10
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.38 0.54 0.42 0.30 0.40 0.52 0.39 0.33 0.35 0.35 0.34 0.25 0.35 0.55 0.45 0.39 0.49 0.48 0.09 0.10 0.09 0.29 0.28 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 2 2 5 1 2 0 0 0 0 0 0 1 3 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 3 7 6 6 10 14
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 40 57 42 26 53 43 77 95 53 76 44 54 79 88 101 47 38 29 12 13 20 43 42 39
Endoplasmic Reticulum 0 0 1 1 1 0 3 4 1 2 3 2 2 0 1 3 4 1 2 0 0 1 1 4
Endosome 0 0 0 1 0 1 1 0 0 0 0 2 1 2 2 0 2 1 1 2 0 2 6 9
Golgi 2 5 24 24 44 5 91 52 20 45 29 29 6 8 11 15 5 0 0 3 0 0 1 1
Mitochondria 0 0 0 0 0 5 2 2 1 2 4 2 0 0 0 2 2 3 0 1 0 1 1 0
Nucleus 17 15 69 37 38 20 58 71 40 45 28 31 34 37 35 13 3 6 75 111 159 104 140 152
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 1 2 5 8
Nucleolus 1 1 0 1 0 5 6 5 2 6 1 4 0 2 4 2 1 1 2 3 3 15 22 36
Peroxisomes 0 1 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 0 0 0 0 0 1 1 0 0 0 0 1 0 1 5 8 9
Vac/Vac Membrane 28 67 76 31 70 68 120 96 56 77 48 38 52 125 105 41 36 30 10 17 18 78 97 98
Unique Cell Count 74 123 180 104 173 130 310 292 159 220 143 154 149 229 234 106 74 62 118 172 217 270 346 385
Labelled Cell Count 88 146 212 121 206 149 359 327 175 258 159 167 176 263 259 123 91 72 118 172 217 270 346 385

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 50.4 44.4 42.1 49.1 41.2 27.7 30.9 28.5 27.5 27.8 27.0 26.3 49.2 41.3 45.9 43.9 42.1 45.8 44.7 47.0 49.5
Std Deviation (1e-4) 19.3 18.6 11.0 17.8 11.5 16.0 8.1 7.8 6.4 8.5 8.1 7.6 15.0 11.3 13.4 19.6 18.3 22.2 15.4 13.3 13.4
Intensity Change (Log2) 0.22 -0.03 -0.6 -0.45 -0.56 -0.61 -0.6 -0.64 -0.68 0.23 -0.03 0.12 0.06 -0.0 0.12 0.09 0.16 0.23

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 1.6 0.4 1.2 2.6 1.9 2.1 2.1 2.5 4.3 1.4 0.3 3.6 3.6 1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 2.1 2.9 -2.8 4.0 1.3 -0.2 1.9 1.7 1.4 -2.9 -3.7 -3.1 0.2 -1.5 -3.0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.1 -2.4 -2.9 -3.0 -4.5 -3.1 -3.5 -4.1 -4.5 -4.2 -1.3 -3.1 -3.6 -3.1 -1.4