Standard name | |
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Description | Zn-ribbon protein that co-purifies with Dph1 and Dph2; in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p; involved in modification of wobble nucleosides in tRNAs; forms a stable heterodimer with Ats1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 1 | – | 5 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Bud | 0 | 0 | 8 | 0 | 0 | 0 | – | 2 | 0 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 5 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 2 | 2 | 3 | – | 4 | 6 | 3 | 7 | 10 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 212 | 128 | 283 | 249 | 289 | 314 | – | 345 | 293 | 227 | 204 | 280 | 324 | 304 | 389 | 96 | 48 | 18 | 194 | 126 | 262 | 213 | 280 | 243 |
Endoplasmic Reticulum | 1 | 2 | 2 | 2 | 4 | 4 | – | 16 | 12 | 3 | 7 | 15 | 3 | 2 | 1 | 5 | 2 | 2 | 0 | 1 | 4 | 1 | 3 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 1 | 1 | – | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 0 | 0 | 1 | 1 | 3 | 6 | – | 24 | 42 | 36 | 52 | 80 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 10 | 0 | 3 | 1 | 8 | – | 23 | 16 | 10 | 13 | 30 | 1 | 4 | 3 | 0 | 0 | 0 | 16 | 20 | 12 | 23 | 44 | 44 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 11 | 3 | 9 | 28 | 30 | – | 163 | 48 | 61 | 56 | 40 | 24 | 19 | 104 | 7 | 3 | 0 | 1 | 6 | 5 | 14 | 27 | 23 |
Unique Cell Count | 213 | 144 | 293 | 252 | 311 | 352 | 551 | 393 | 319 | 323 | 429 | 333 | 314 | 450 | 99 | 50 | 18 | 218 | 164 | 297 | 258 | 378 | 339 | |
Labelled Cell Count | 214 | 152 | 298 | 266 | 328 | 367 | 588 | 419 | 341 | 347 | 461 | 353 | 329 | 503 | 110 | 53 | 20 | 218 | 164 | 297 | 258 | 378 | 339 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 27.0 | 21.3 | 18.9 | 23.5 | 20.0 | 15.9 | – | 14.4 | 13.4 | 13.2 | 12.9 | 12.3 | 28.9 | 27.1 | 25.4 | 45.3 | 41.1 | 43.6 | 29.7 | 28.9 | 28.4 |
Std Deviation (1e-4) | 5.5 | 5.5 | 4.5 | 4.4 | 3.8 | 2.8 | – | 2.9 | 2.9 | 2.9 | 3.4 | 2.8 | 5.5 | 6.0 | 5.4 | 17.1 | 15.9 | 12.5 | 5.8 | 6.8 | 7.3 |
Intensity Change (Log2) | – | – | – | 0.31 | 0.08 | -0.25 | – | -0.4 | -0.5 | -0.52 | -0.55 | -0.62 | 0.61 | 0.52 | 0.42 | 1.26 | 1.12 | 1.2 | 0.65 | 0.61 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | -1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.7 | -2.0 | -3.6 | – | -10.8 | -7.8 | -8.4 | -10.2 | -10.0 | 0.5 | 0.2 | -4.6 | 0.2 | -0.2 | 0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 2.1 | 2.2 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 3.3 | 5.5 | 5.7 | 7.0 | 7.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 3.5 | 3.5 | 0 | 3.5 | 4.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.0 | 4.4 | 4.3 | – | 9.9 | 5.5 | 7.3 | 6.9 | 4.6 | 3.8 | 3.3 | 8.4 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 17.5412 | 21.4798 | 19.8013 | 19.2768 | 18.9844 | 20.0095 | 16.7519 | 20.2213 | 18.2849 | 18.762 | 18.9273 | 17.9835 | 22.535 | 24.3867 | 22.42 | 22.5284 | 21.463 | 21.4962 |
Actin | 0.0049 | 0.0004 | 0.0074 | 0.0063 | 0.0012 | 0.0024 | 0.0236 | 0.0001 | 0.0114 | 0.0002 | 0.0001 | 0.003 | 0.0034 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0005 |
Bud | 0.0007 | 0.0008 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0011 | 0.0003 | 0.0004 | 0.0004 | 0.0008 | 0.0012 | 0.0013 | 0.0002 | 0.0005 | 0.0003 | 0.0006 | 0.0011 | 0.0005 | 0.0003 | 0.0004 | 0.0005 | 0.0008 | 0.0011 |
Bud Periphery | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0002 | 0.0003 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0008 | 0.0002 | 0.0003 | 0.0003 | 0.0006 | 0.0011 | 0.0008 | 0 | 0.0006 | 0 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cell Periphery | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5974 | 0.7931 | 0.7144 | 0.6665 | 0.6732 | 0.5692 | 0.5286 | 0.7753 | 0.6639 | 0.6238 | 0.8239 | 0.5996 | 0.5739 | 0.7471 | 0.6121 | 0.5875 | 0.6219 | 0.54 |
Cytoplasmic Foci | 0.0122 | 0.0004 | 0.0032 | 0.0026 | 0.0068 | 0.0019 | 0.0196 | 0.0001 | 0.0051 | 0.0004 | 0.0005 | 0.0031 | 0.0015 | 0.0002 | 0.0036 | 0.0004 | 0.0004 | 0.0007 |
Eisosomes | 0.0001 | 0 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0081 | 0.0012 | 0.0028 | 0.0016 | 0.0085 | 0.0015 | 0.0054 | 0.0006 | 0.0029 | 0.0013 | 0.001 | 0.0025 | 0.0015 | 0.0007 | 0.0013 | 0.0009 | 0.0019 | 0.002 |
Endosome | 0.0152 | 0.0001 | 0.0032 | 0.0025 | 0.0102 | 0.002 | 0.0236 | 0.0001 | 0.0096 | 0.0005 | 0.0002 | 0.0127 | 0.0014 | 0.0001 | 0.0023 | 0.0008 | 0.0008 | 0.0032 |
Golgi | 0.0016 | 0 | 0.0014 | 0.0013 | 0.0009 | 0.0006 | 0.008 | 0 | 0.0045 | 0 | 0 | 0.0081 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0.0003 |
Lipid Particles | 0.0058 | 0 | 0.0012 | 0.0073 | 0.0027 | 0.001 | 0.0059 | 0 | 0.0021 | 0 | 0 | 0.001 | 0.0003 | 0 | 0.0009 | 0 | 0 | 0.0002 |
Mitochondria | 0.0057 | 0.0005 | 0.0011 | 0.0157 | 0.0034 | 0.0036 | 0.0032 | 0.0001 | 0.005 | 0.0001 | 0.0001 | 0.0054 | 0.0057 | 0.0002 | 0.0006 | 0.0002 | 0.0001 | 0.0003 |
None | 0.004 | 0.0003 | 0.0013 | 0.0022 | 0.0021 | 0.0004 | 0.004 | 0.0002 | 0.001 | 0.0002 | 0.0004 | 0.0004 | 0.001 | 0.0002 | 0.0006 | 0.0003 | 0.0003 | 0.0004 |
Nuclear Periphery | 0.0316 | 0.0097 | 0.0159 | 0.0171 | 0.0252 | 0.0223 | 0.0268 | 0.0115 | 0.0146 | 0.023 | 0.0144 | 0.0212 | 0.021 | 0.0109 | 0.0163 | 0.0155 | 0.0212 | 0.0218 |
Nucleolus | 0.0017 | 0.0001 | 0.0002 | 0.0004 | 0.001 | 0.0011 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Nucleus | 0.2916 | 0.1911 | 0.242 | 0.2697 | 0.2403 | 0.385 | 0.3257 | 0.2106 | 0.2742 | 0.3474 | 0.1566 | 0.3367 | 0.3819 | 0.2391 | 0.3593 | 0.3908 | 0.3488 | 0.4261 |
Peroxisomes | 0.0015 | 0 | 0.001 | 0.0018 | 0.0004 | 0.0006 | 0.0022 | 0 | 0.0004 | 0 | 0 | 0.0004 | 0.0009 | 0 | 0.0001 | 0 | 0 | 0 |
Punctate Nuclear | 0.0081 | 0.001 | 0.002 | 0.0018 | 0.0138 | 0.0045 | 0.0148 | 0.0004 | 0.0015 | 0.0018 | 0.0013 | 0.0022 | 0.0038 | 0.0006 | 0.0012 | 0.0016 | 0.0026 | 0.0019 |
Vacuole | 0.0051 | 0.0004 | 0.0013 | 0.0009 | 0.0057 | 0.0009 | 0.0039 | 0.0004 | 0.0014 | 0.0005 | 0.0005 | 0.0013 | 0.0009 | 0.0003 | 0.0005 | 0.0007 | 0.0006 | 0.001 |
Vacuole Periphery | 0.002 | 0.0001 | 0.0004 | 0.0007 | 0.0015 | 0.0004 | 0.0011 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 70.9015 | 106.9565 | 82.3058 | 49.5656 | 45.9634 | 84.1489 | 65.0864 | 45.9564 | 62.0889 | 58.4269 |
Translational Efficiency | 1.4832 | 1.2209 | 1.5148 | 2.6896 | 2.7252 | 1.3627 | 2.0559 | 3.3676 | 2.1875 | 2.0232 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |