Standard name
Description Zn-ribbon protein that co-purifies with Dph1 and Dph2; in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p; involved in modification of wobble nucleosides in tRNAs; forms a stable heterodimer with Ats1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.89 0.97 0.99 0.93 0.89 – 0.63 0.75 0.71 0.63 0.65 0.97 0.97 0.86 0.97 0.96 1.0 0.89 0.77 0.89 0.83 0.74 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0.11 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0.11 0.11 0.16 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.07 0 0 0 0 – 0 0 0 0 0.07 0 0 0 0 0 0 0.08 0.13 0 0.09 0.12 0.13
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 0 0 0.09 0.09 – 0.3 0.12 0.19 0.17 0.09 0.07 0.06 0.23 0.07 0.06 0 0 0 0 0.06 0.07 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 1 – 5 0 0 1 2 0 0 0 0 0 0 0 0 1 0 1 0
Bud 0 0 8 0 0 0 – 2 0 0 3 2 1 0 0 0 0 0 0 1 1 0 5 7
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 2 2 3 – 4 6 3 7 10 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 212 128 283 249 289 314 – 345 293 227 204 280 324 304 389 96 48 18 194 126 262 213 280 243
Endoplasmic Reticulum 1 2 2 2 4 4 – 16 12 3 7 15 3 2 1 5 2 2 0 1 4 1 3 3
Endosome 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 2
Golgi 0 0 0 0 1 1 – 3 2 0 0 0 0 0 5 0 0 0 0 0 0 0 0 2
Mitochondria 0 0 1 1 3 6 – 24 42 36 52 80 0 0 0 1 0 0 0 0 1 0 0 0
Nucleus 0 10 0 3 1 8 – 23 16 10 13 30 1 4 3 0 0 0 16 20 12 23 44 44
Nuclear Periphery 0 0 0 0 0 0 – 0 0 1 1 1 0 0 0 0 0 0 0 0 2 0 3 2
Nucleolus 0 0 0 0 0 0 – 2 0 0 2 1 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 11 3 9 28 30 – 163 48 61 56 40 24 19 104 7 3 0 1 6 5 14 27 23
Unique Cell Count 213 144 293 252 311 352 551 393 319 323 429 333 314 450 99 50 18 218 164 297 258 378 339
Labelled Cell Count 214 152 298 266 328 367 588 419 341 347 461 353 329 503 110 53 20 218 164 297 258 378 339


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 27.0 21.3 18.9 23.5 20.0 15.9 – 14.4 13.4 13.2 12.9 12.3 28.9 27.1 25.4 45.3 41.1 43.6 29.7 28.9 28.4
Std Deviation (1e-4) 5.5 5.5 4.5 4.4 3.8 2.8 – 2.9 2.9 2.9 3.4 2.8 5.5 6.0 5.4 17.1 15.9 12.5 5.8 6.8 7.3
Intensity Change (Log2) – – – 0.31 0.08 -0.25 – -0.4 -0.5 -0.52 -0.55 -0.62 0.61 0.52 0.42 1.26 1.12 1.2 0.65 0.61 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 -1.7 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.7 -2.0 -3.6 – -10.8 -7.8 -8.4 -10.2 -10.0 0.5 0.2 -4.6 0.2 -0.2 0.8
Endoplasmic Reticulum 0 0 0 – 2.1 2.2 0 0 2.4 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 3.3 5.5 5.7 7.0 7.7 0 0 0 0 0 0
Nucleus 0 0 0 – 3.5 3.5 0 3.5 4.6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.0 4.4 4.3 – 9.9 5.5 7.3 6.9 4.6 3.8 3.3 8.4 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.5412 21.4798 19.8013 19.2768 18.9844 20.0095 16.7519 20.2213 18.2849 18.762 18.9273 17.9835 22.535 24.3867 22.42 22.5284 21.463 21.4962
Actin 0.0049 0.0004 0.0074 0.0063 0.0012 0.0024 0.0236 0.0001 0.0114 0.0002 0.0001 0.003 0.0034 0.0001 0.0003 0.0002 0.0003 0.0005
Bud 0.0007 0.0008 0.0002 0.0001 0.0006 0.0002 0.0004 0.0001 0.0004 0 0.0001 0.0002 0.0005 0.0001 0.0001 0.0001 0 0.0001
Bud Neck 0.0011 0.0003 0.0004 0.0004 0.0008 0.0012 0.0013 0.0002 0.0005 0.0003 0.0006 0.0011 0.0005 0.0003 0.0004 0.0005 0.0008 0.0011
Bud Periphery 0.0006 0.0001 0.0002 0.0001 0.0009 0.0002 0.0003 0 0.0005 0 0 0.0001 0.0005 0 0.0001 0 0 0
Bud Site 0.0008 0.0002 0.0003 0.0003 0.0006 0.0011 0.0008 0 0.0006 0 0 0.0001 0.0004 0.0001 0.0001 0 0 0
Cell Periphery 0.0002 0 0 0.0001 0.0002 0 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5974 0.7931 0.7144 0.6665 0.6732 0.5692 0.5286 0.7753 0.6639 0.6238 0.8239 0.5996 0.5739 0.7471 0.6121 0.5875 0.6219 0.54
Cytoplasmic Foci 0.0122 0.0004 0.0032 0.0026 0.0068 0.0019 0.0196 0.0001 0.0051 0.0004 0.0005 0.0031 0.0015 0.0002 0.0036 0.0004 0.0004 0.0007
Eisosomes 0.0001 0 0 0.0004 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0081 0.0012 0.0028 0.0016 0.0085 0.0015 0.0054 0.0006 0.0029 0.0013 0.001 0.0025 0.0015 0.0007 0.0013 0.0009 0.0019 0.002
Endosome 0.0152 0.0001 0.0032 0.0025 0.0102 0.002 0.0236 0.0001 0.0096 0.0005 0.0002 0.0127 0.0014 0.0001 0.0023 0.0008 0.0008 0.0032
Golgi 0.0016 0 0.0014 0.0013 0.0009 0.0006 0.008 0 0.0045 0 0 0.0081 0.0003 0 0.0001 0 0 0.0003
Lipid Particles 0.0058 0 0.0012 0.0073 0.0027 0.001 0.0059 0 0.0021 0 0 0.001 0.0003 0 0.0009 0 0 0.0002
Mitochondria 0.0057 0.0005 0.0011 0.0157 0.0034 0.0036 0.0032 0.0001 0.005 0.0001 0.0001 0.0054 0.0057 0.0002 0.0006 0.0002 0.0001 0.0003
None 0.004 0.0003 0.0013 0.0022 0.0021 0.0004 0.004 0.0002 0.001 0.0002 0.0004 0.0004 0.001 0.0002 0.0006 0.0003 0.0003 0.0004
Nuclear Periphery 0.0316 0.0097 0.0159 0.0171 0.0252 0.0223 0.0268 0.0115 0.0146 0.023 0.0144 0.0212 0.021 0.0109 0.0163 0.0155 0.0212 0.0218
Nucleolus 0.0017 0.0001 0.0002 0.0004 0.001 0.0011 0.0005 0.0001 0.0001 0.0001 0 0.0001 0.0005 0 0.0001 0.0001 0.0001 0.0002
Nucleus 0.2916 0.1911 0.242 0.2697 0.2403 0.385 0.3257 0.2106 0.2742 0.3474 0.1566 0.3367 0.3819 0.2391 0.3593 0.3908 0.3488 0.4261
Peroxisomes 0.0015 0 0.001 0.0018 0.0004 0.0006 0.0022 0 0.0004 0 0 0.0004 0.0009 0 0.0001 0 0 0
Punctate Nuclear 0.0081 0.001 0.002 0.0018 0.0138 0.0045 0.0148 0.0004 0.0015 0.0018 0.0013 0.0022 0.0038 0.0006 0.0012 0.0016 0.0026 0.0019
Vacuole 0.0051 0.0004 0.0013 0.0009 0.0057 0.0009 0.0039 0.0004 0.0014 0.0005 0.0005 0.0013 0.0009 0.0003 0.0005 0.0007 0.0006 0.001
Vacuole Periphery 0.002 0.0001 0.0004 0.0007 0.0015 0.0004 0.0011 0.0001 0.0004 0.0002 0.0001 0.0007 0.0003 0.0001 0.0002 0.0003 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 70.9015 106.9565 82.3058 49.5656 45.9634 84.1489 65.0864 45.9564 62.0889 58.4269
Translational Efficiency 1.4832 1.2209 1.5148 2.6896 2.7252 1.3627 2.0559 3.3676 2.1875 2.0232

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘