Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0 0 0.06 0 0.1 0 0 0.05 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.97 0.92 0.92 0.9 0.87 0.79 0.8 0.78 0.77 0.78 0.97 0.96 0.98 0.98 1.0 1.0 0.95 0.91 0.92 0.9 0.91 0.91
Endoplasmic Reticulum 0 0 0 0.05 0 0.05 0.05 0.14 0.09 0.09 0.06 0.1 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.06 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 3 2 3 7 14 8 13 7 11 2 0 0 0 0 0 0 0 0 0 0 1
Bud 1 1 0 1 0 0 3 3 1 3 3 2 1 0 1 0 0 0 0 2 1 7 16 18
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 0 0 4 9 10 16 19 7 16 4 18 6 19 22 3 1 1 0 0 0 0 0 0
Cytoplasm 137 155 174 185 237 227 278 339 144 193 148 145 397 546 477 267 234 231 138 147 166 290 406 402
Endoplasmic Reticulum 6 5 5 11 8 13 15 61 17 22 12 18 18 14 2 13 16 9 2 1 3 9 5 10
Endosome 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Golgi 1 0 0 5 0 6 8 14 5 7 6 6 0 6 6 1 0 0 0 1 0 0 0 0
Mitochondria 0 1 0 1 1 0 10 10 8 9 11 4 0 0 0 1 0 0 0 0 0 0 1 0
Nucleus 0 0 0 0 1 0 1 1 0 2 1 0 1 0 0 0 0 0 0 1 0 0 2 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1
Vac/Vac Membrane 0 0 2 3 10 4 3 16 11 11 11 9 3 3 1 1 1 1 0 5 4 5 5 3
Unique Cell Count 140 160 180 201 258 252 319 428 179 248 191 187 409 568 485 273 234 232 145 163 180 323 449 444
Labelled Cell Count 148 162 181 213 268 263 341 477 201 276 204 213 428 588 509 286 252 242 145 163 180 323 449 444


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 63.7 80.1 59.9 66.2 49.3 35.8 31.3 28.5 25.0 25.6 24.6 27.0 60.5 58.1 57.0 175.4 180.3 172.1 63.9 64.0 64.1
Std Deviation (1e-4) 35.2 50.8 35.0 38.2 27.7 20.7 17.3 15.5 14.2 13.7 14.0 14.7 38.8 36.6 35.5 55.6 60.8 61.4 40.7 40.5 40.4
Intensity Change (Log2) – – – 0.14 -0.28 -0.74 -0.94 -1.07 -1.26 -1.23 -1.28 -1.15 0.01 -0.04 -0.07 1.55 1.59 1.52 0.09 0.1 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 3.1 0 3.3 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.1 2.9 0 3.5 0 4.3 0 0 2.9 0 0 0
Cytoplasm -1.9 -2.1 -2.6 -3.5 -5.4 -4.8 -5.5 -5.5 -5.4 0.3 -0.3 1.3 0.7 2.8 2.3
Endoplasmic Reticulum 1.3 0.2 1.2 1.1 4.2 2.7 2.6 1.6 2.7 0.9 0 0 1.1 1.9 0.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3.3 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.7 2.6 2.0 2.4 2.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 60.7286 77.9037 74.6911 61.8647 59.9554 77.082 53.7733 63.1834 58.596 57.3699 42.8054 57.3033 70.6352 88.2791 73.6984 73.6897 70.5594 88.3153
Actin 0.0008 0.003 0.0006 0.0001 0.0008 0.0007 0.0022 0.0001 0.0022 0.0001 0.0027 0.0093 0.0005 0.0014 0.0006 0.0004 0.0034 0.0021
Bud 0.0022 0.0042 0.0039 0.0011 0.0013 0.0012 0.0022 0.0004 0.0012 0.0002 0.0006 0.0011 0.0021 0.0032 0.0037 0.005 0.0059 0.0022
Bud Neck 0.0004 0.0006 0.001 0.0006 0.0006 0.0012 0.0005 0.0002 0.0036 0.0002 0.0004 0.0016 0.0004 0.0003 0.001 0.0005 0.0008 0.0024
Bud Periphery 0.0023 0.0032 0.0018 0.0008 0.0007 0.0008 0.0017 0.0007 0.0025 0.0001 0.0009 0.0005 0.0015 0.0011 0.0015 0.0052 0.0022 0.002
Bud Site 0.001 0.0054 0.002 0.0003 0.0004 0.0002 0.0023 0.0002 0.0009 0.0001 0.0005 0.0001 0.0004 0.0041 0.0008 0.0005 0.0013 0.0002
Cell Periphery 0.0219 0.0083 0.0128 0.0144 0.0111 0.0085 0.0052 0.001 0.0031 0.0007 0.0006 0.0013 0.0128 0.0043 0.0095 0.0017 0.0025 0.0065
Cytoplasm 0.7802 0.9232 0.8699 0.8117 0.8186 0.8485 0.8384 0.9813 0.9283 0.9056 0.812 0.9193 0.8303 0.9468 0.8922 0.8327 0.8868 0.842
Cytoplasmic Foci 0.0064 0.0017 0.0031 0.0027 0.0117 0.0069 0.0124 0.001 0.0041 0.0038 0.0388 0.006 0.0039 0.0045 0.004 0.0052 0.0057 0.0048
Eisosomes 0.0003 0.0001 0 0 0 0.0002 0.0003 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0224 0.0079 0.0041 0.0111 0.0048 0.0481 0.0191 0.0029 0.0093 0.0215 0.0076 0.0067 0.033 0.0031 0.0114 0.0027 0.0058 0.0347
Endosome 0.0095 0.0022 0.007 0.0037 0.0415 0.0159 0.019 0.0011 0.0061 0.034 0.0291 0.0149 0.0132 0.004 0.0099 0.0086 0.0155 0.0165
Golgi 0.0012 0.0003 0.0007 0.0002 0.0052 0.0031 0.0045 0.0002 0.0007 0.0015 0.0062 0.0031 0.0014 0.0013 0.0007 0.0002 0.001 0.002
Lipid Particles 0.0023 0.0005 0.0013 0.0023 0.0022 0.0011 0.0072 0.0002 0.0006 0.0003 0.0225 0.0006 0.0013 0.0007 0.001 0.0002 0.0007 0.0014
Mitochondria 0.0067 0.0005 0.0018 0.0006 0.0018 0.0015 0.0174 0.0002 0.0019 0.0004 0.0298 0.0025 0.0016 0.0008 0.0011 0.0019 0.0006 0.0012
None 0.0059 0.0007 0.0011 0.0007 0.0013 0.0009 0.0048 0.0002 0.0012 0.0003 0.0092 0.0009 0.0008 0.0005 0.0011 0.0014 0.0005 0.0007
Nuclear Periphery 0.0076 0.0017 0.0035 0.0047 0.0061 0.0061 0.0039 0.0007 0.0024 0.0024 0.005 0.0015 0.0165 0.0012 0.0052 0.0048 0.0031 0.02
Nucleolus 0.0099 0.0002 0.0006 0.003 0.0045 0.0004 0.002 0.0001 0.0005 0.0001 0.0048 0.0003 0.0005 0.0003 0.0008 0.0084 0.0002 0.0005
Nucleus 0.0063 0.0019 0.0033 0.0052 0.0059 0.0026 0.004 0.0006 0.0032 0.0012 0.0043 0.0014 0.0036 0.0014 0.0053 0.0058 0.0021 0.0045
Peroxisomes 0.0003 0.0001 0.0006 0.0001 0.0001 0.0001 0.0019 0 0.0002 0 0.0015 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Punctate Nuclear 0.0004 0.0001 0.0002 0.0001 0.0002 0.0003 0.0018 0 0.0001 0 0.0032 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002
Vacuole 0.096 0.0317 0.0736 0.118 0.0607 0.04 0.0409 0.0073 0.0239 0.0232 0.0133 0.0222 0.0617 0.0183 0.0427 0.0862 0.0495 0.0411
Vacuole Periphery 0.0162 0.0026 0.0071 0.0186 0.0207 0.0118 0.0084 0.0017 0.0041 0.0042 0.0065 0.0064 0.0143 0.0026 0.0071 0.0284 0.0119 0.015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2644.2991 3081.9929 3550.5204 2529.0617 3326.8006 3242.6001 2498.5589 2319.3339 2808.7746 2192.9477
Translational Efficiency 1.244 1.1436 1.0321 1.5378 1.3204 1.0668 1.1736 1.438 1.1096 1.4799

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl19b

Rpl19b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl19b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available