Standard name
Description Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.11 0.13 0.14 0.1 0.18 0.28 0.18
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0 0.06 0.06 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.17 0.24 0.17 0.11 0.13 0.11 0.2 0.19 0.19
Mitochondria 0.78 0.78 0.74 0.71 0.76 0.84 0.79 0.82 0.88 0.9 0.92 0.9 0.8 0.74 0.7 0.2 0.16 0.11 0.45 0.42 0.39 0.32 0.26 0.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.24 0.21 0.22 0.2 0.28 0.32 0.33 0.32 0.29 0.23 0.22 0.26 0.16 0.16 0.21 0.28 0.32 0.28 0.05 0.05 0 0.05 0 0
Peroxisomes 0 0.07 0.22 0.21 0.14 0.05 0.07 0 0 0 0 0 0.14 0.11 0.09 0.08 0.14 0.11 0.06 0.09 0.27 0.18 0.2 0.25
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.19 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.11 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 4 1 0 0 0 0 1 0 2 6 0 2 6 4 4 2 4 18 61 17 34 67 50
Bud 0 1 1 0 0 4 1 1 3 6 2 3 2 4 1 0 1 0 1 9 1 1 2 2
Bud Neck 0 1 0 1 0 0 1 0 0 1 0 0 1 0 0 0 0 0 1 5 1 0 0 1
Bud Site 1 0 0 0 1 2 2 0 0 2 3 0 0 1 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0 0
Cytoplasm 4 1 1 3 3 4 6 4 5 1 4 0 2 2 1 9 1 2 7 1 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 1 0
Endosome 0 2 1 1 3 0 2 0 0 1 0 0 1 2 0 0 2 1 3 19 3 3 4 0
Golgi 1 7 1 1 0 0 0 0 0 0 0 0 1 4 23 11 9 6 14 58 17 37 45 52
Mitochondria 66 253 102 67 139 192 120 218 215 294 231 206 96 117 114 13 6 4 62 186 66 61 63 83
Nucleus 3 5 1 0 1 0 3 0 2 1 1 0 0 0 0 1 1 0 0 0 0 0 0 0
Nuclear Periphery 1 2 0 0 0 0 0 2 1 1 0 1 0 1 0 1 0 0 0 5 1 0 0 0
Nucleolus 20 67 30 19 51 73 50 86 70 76 55 60 19 26 35 18 12 10 6 20 3 8 4 7
Peroxisomes 0 24 31 20 25 11 11 10 4 9 3 5 17 18 14 5 5 4 8 37 45 34 49 70
SpindlePole 0 2 4 1 3 1 6 1 0 3 1 3 1 5 1 1 2 7 4 12 4 2 1 1
Vac/Vac Membrane 2 8 2 0 2 1 0 3 1 5 2 1 3 2 7 4 4 4 0 12 1 2 1 4
Unique Cell Count 85 326 138 95 183 228 152 267 243 328 252 230 120 159 164 64 37 36 139 440 169 190 244 278
Labelled Cell Count 98 377 175 113 228 288 202 326 301 402 308 279 145 188 202 70 45 42 139 440 169 190 244 278


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 39.1 33.0 24.2 25.9 27.0 26.2 24.7 31.0 30.3 32.1 31.4 32.4 32.6 32.9 36.8 30.5 33.5 26.8 44.4 46.3 44.4
Std Deviation (1e-4) 21.5 15.2 10.5 11.2 11.4 11.1 9.5 11.9 11.3 12.8 11.6 13.0 14.8 12.0 13.9 13.4 11.3 10.3 20.1 25.4 21.0
Intensity Change (Log2) 0.1 0.16 0.12 0.03 0.36 0.32 0.41 0.38 0.42 0.43 0.44 0.6 0.33 0.47 0.15 0.88 0.93 0.88


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 4.3 0 0 0
Mitochondria -0.6 0.4 2.4 1.0 1.8 3.7 4.3 4.7 3.9 1.2 -0.1 -0.8 -7.2 -6.4 -6.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.3 1.3 2.1 2.1 2.2 1.5 0.3 0 0.9 -1.2 -1.2 -0.1 1.0 1.4 0.8
Peroxisomes -0.3 -2.1 -5.1 -3.7 -5.9 -6.8 -6.9 -7.1 -6.3 -1.7 -2.6 -3.4 -2.5 -1.2 -1.5
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atp3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available