Standard name
Description Ubiquitin-specific protease; specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.29 0.35 0.4 0.19 0.25 0.18 0.16 0.17 0.18 0.16 0.1 0.08 0.33 0.31 0.32 0.38 0.29 0.28 0.23 0.26 0.31 0.26 0.24 0.32
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0.17 0 0.2 0 0.31 0.38 0.29 0.59 0.61 0.7 0.54 0.05 0 0.06 0 0 0 0 0 0 0 0 0
Nucleus 0.31 0.27 0.23 0.56 0.61 0.59 0.63 0.62 0.61 0.51 0.57 0.68 0.29 0.2 0.24 0.31 0.33 0.31 0.47 0.42 0.28 0.32 0.27 0.21
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.42 0.46 0.38 0.15 0.17 0.11 0.08 0.05 0 0 0 0.05 0.53 0.59 0.46 0.38 0.46 0.44 0.23 0.21 0.33 0.32 0.34 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 4
Bud 0 0 2 1 0 1 3 2 1 10 11 6 0 0 1 0 0 1 0 2 1 4 9 10
Bud Neck 0 0 0 0 1 0 0 5 0 0 3 1 0 0 0 0 0 0 0 1 1 0 4 7
Bud Site 0 0 0 0 0 2 1 4 5 5 10 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 32 74 55 17 31 37 41 53 25 33 27 18 53 65 74 61 50 44 29 70 61 39 57 93
Endoplasmic Reticulum 1 1 0 0 1 1 0 0 0 0 0 0 0 0 0 6 2 13 0 0 0 0 1 4
Endosome 0 2 3 0 3 4 1 3 1 0 0 0 4 9 11 4 7 3 1 4 0 0 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 3 0 1 0 0 0 0
Mitochondria 22 36 6 18 3 63 100 88 82 128 193 118 8 2 13 3 2 5 2 6 5 2 8 9
Nucleus 34 57 31 50 77 120 165 191 85 108 156 147 47 42 56 49 56 50 58 113 55 46 64 61
Nuclear Periphery 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 1 1 0 0 1 3 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 8 0 2 0 2 0 0 0 0 0 0 1 7 3 2 3 5
Vac/Vac Membrane 46 97 51 13 21 22 20 16 3 4 7 10 86 125 107 60 79 71 28 58 65 48 81 86
Unique Cell Count 109 213 136 89 126 202 263 307 139 211 276 217 162 211 233 160 172 160 124 272 197 148 238 289
Labelled Cell Count 136 268 149 100 137 250 331 370 203 292 409 309 198 244 265 185 197 191 124 272 197 148 238 289


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.3 5.0 4.8 5.8 4.4 4.6 4.9 4.2 3.9 3.8 4.1 5.2 5.4 5.4 7.1 7.2 7.1 4.7 4.7 5.2
Std Deviation (1e-4) 0.6 0.7 0.7 1.4 2.1 1.1 1.1 1.3 1.6 1.3 1.0 1.1 0.8 1.3 1.4 1.5 1.5 1.4 1.1 1.1 1.5
Intensity Change (Log2) – – – -0.05 0.2 -0.19 -0.13 -0.04 -0.25 -0.35 -0.41 -0.3 0.05 0.09 0.11 0.5 0.53 0.49 -0.11 -0.11 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 1.5 1.2