Standard name
Description Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0.06 0.07 0.06 0.07 0.06 0.07 0.06 0.12 0.18 0.23 0.06 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0 0 0 0.17 0.26 0.13 0.37 0.35 0.62 0.43 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.92 0.9 0.92 0.89 0.83 0.81 0.73 0.82 0.69 0.73 0.63 0.68 0.87 0.76 0.73 0.83 0.81 0.79 0.81 0.82 0.82 0.79 0.74 0.55
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.06 0.05 0.06 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0.05 0.06 0 0.05 0.05 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.05 0.05 0.06 0 0.08 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 2
Bud 1 0 0 0 1 0 4 8 6 2 2 5 0 0 0 2 0 0 3 2 0 0 0 1
Bud Neck 0 0 1 0 3 3 5 9 2 5 2 2 1 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 3 3 6 2 6 12 11 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 16 15 10 23 24 35 32 32 25 29 20 29 43 61 25 26 24 2 2 2 1 3 4
Endoplasmic Reticulum 2 1 0 0 1 0 1 1 0 0 0 0 0 0 0 9 14 6 0 1 1 0 0 0
Endosome 0 3 0 4 8 6 5 2 3 4 0 1 0 6 7 8 6 11 2 8 3 1 3 11
Golgi 1 2 1 0 0 1 0 0 1 0 0 0 1 0 0 2 2 1 5 3 1 0 0 0
Mitochondria 62 6 3 10 7 63 132 74 162 146 259 141 0 0 1 18 9 7 5 4 2 2 2 7
Nucleus 366 379 345 279 316 306 366 455 303 304 262 222 208 183 196 328 315 276 350 377 323 63 128 126
Nuclear Periphery 0 2 0 1 2 0 5 1 2 1 1 2 0 0 2 2 5 0 0 1 0 0 0 2
Nucleolus 13 5 0 6 7 7 4 7 4 5 4 3 2 6 3 4 11 4 23 24 23 4 10 28
Peroxisomes 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 0 0 1 0 0 0 0 0
SpindlePole 2 2 17 16 24 12 23 28 21 25 5 6 10 4 5 10 6 6 8 6 5 0 3 5
Vac/Vac Membrane 4 15 10 3 13 10 12 9 16 13 8 13 0 7 6 17 29 35 19 25 22 3 14 32
Unique Cell Count 397 423 377 314 380 377 502 556 437 417 415 327 240 240 269 395 388 351 431 461 395 81 175 230
Labelled Cell Count 459 431 392 330 410 435 598 628 559 542 584 422 251 249 282 425 423 370 431 461 395 81 175 230


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 6.3 6.3 5.8 5.7 5.0 5.0 5.1 4.4 4.4 3.9 4.1 6.3 6.7 6.3 7.1 7.6 7.2 5.5 6.8 7.0
Std Deviation (1e-4) 0.8 1.0 1.1 1.1 1.3 1.3 1.4 1.2 1.3 0.7 1.2 0.8 1.3 1.7 1.6 1.3 1.6 1.6 1.0 1.4 1.4
Intensity Change (Log2) – – – -0.11 -0.14 -0.34 -0.34 -0.3 -0.51 -0.54 -0.68 -0.61 0.01 0.08 -0.01 0.18 0.27 0.19 -0.2 0.11 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 1.3 1.5 1.9 1.2 2.0 1.3 1.8 1.3 3.8 5.8 7.3 1.5 1.7 1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.7 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.3 0 7.7 10.4 6.8 0 0 0 0 0 0 0 3.2 1.7 0
Nucleus -1.2 -3.5 -4.1 -6.9 -4.1 -7.8 -6.8 -9.4 -7.9 -1.9 -5.3 -6.3 -3.5 -4.1 -4.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 3.3 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.4 1.1 -0.9 0.1 0.4 0 0 0 0 -0.2 -1.9 -1.8 -1.5 -2.4 -2.2
Vacuole -1.6 0.6 0 -0.2 -1.1 0.8 0.4 -0.7 1.0 0 0.2 -0.3 1.2 3.0 4.1