Standard name | |
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Description | Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 2 |
Bud | 1 | 0 | 0 | 0 | 1 | 0 | 4 | 8 | 6 | 2 | 2 | 5 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 1 | 0 | 3 | 3 | 5 | 9 | 2 | 5 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 3 | 3 | 6 | 2 | 6 | 12 | 11 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 16 | 15 | 10 | 23 | 24 | 35 | 32 | 32 | 25 | 29 | 20 | 29 | 43 | 61 | 25 | 26 | 24 | 2 | 2 | 2 | 1 | 3 | 4 |
Endoplasmic Reticulum | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 14 | 6 | 0 | 1 | 1 | 0 | 0 | 0 |
Endosome | 0 | 3 | 0 | 4 | 8 | 6 | 5 | 2 | 3 | 4 | 0 | 1 | 0 | 6 | 7 | 8 | 6 | 11 | 2 | 8 | 3 | 1 | 3 | 11 |
Golgi | 1 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 1 | 5 | 3 | 1 | 0 | 0 | 0 |
Mitochondria | 62 | 6 | 3 | 10 | 7 | 63 | 132 | 74 | 162 | 146 | 259 | 141 | 0 | 0 | 1 | 18 | 9 | 7 | 5 | 4 | 2 | 2 | 2 | 7 |
Nucleus | 366 | 379 | 345 | 279 | 316 | 306 | 366 | 455 | 303 | 304 | 262 | 222 | 208 | 183 | 196 | 328 | 315 | 276 | 350 | 377 | 323 | 63 | 128 | 126 |
Nuclear Periphery | 0 | 2 | 0 | 1 | 2 | 0 | 5 | 1 | 2 | 1 | 1 | 2 | 0 | 0 | 2 | 2 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
Nucleolus | 13 | 5 | 0 | 6 | 7 | 7 | 4 | 7 | 4 | 5 | 4 | 3 | 2 | 6 | 3 | 4 | 11 | 4 | 23 | 24 | 23 | 4 | 10 | 28 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 2 | 17 | 16 | 24 | 12 | 23 | 28 | 21 | 25 | 5 | 6 | 10 | 4 | 5 | 10 | 6 | 6 | 8 | 6 | 5 | 0 | 3 | 5 |
Vac/Vac Membrane | 4 | 15 | 10 | 3 | 13 | 10 | 12 | 9 | 16 | 13 | 8 | 13 | 0 | 7 | 6 | 17 | 29 | 35 | 19 | 25 | 22 | 3 | 14 | 32 |
Unique Cell Count | 397 | 423 | 377 | 314 | 380 | 377 | 502 | 556 | 437 | 417 | 415 | 327 | 240 | 240 | 269 | 395 | 388 | 351 | 431 | 461 | 395 | 81 | 175 | 230 |
Labelled Cell Count | 459 | 431 | 392 | 330 | 410 | 435 | 598 | 628 | 559 | 542 | 584 | 422 | 251 | 249 | 282 | 425 | 423 | 370 | 431 | 461 | 395 | 81 | 175 | 230 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 6.3 | 6.3 | 5.8 | 5.7 | 5.0 | 5.0 | 5.1 | 4.4 | 4.4 | 3.9 | 4.1 | 6.3 | 6.7 | 6.3 | 7.1 | 7.6 | 7.2 | 5.5 | 6.8 | 7.0 |
Std Deviation (1e-4) | 0.8 | 1.0 | 1.1 | 1.1 | 1.3 | 1.3 | 1.4 | 1.2 | 1.3 | 0.7 | 1.2 | 0.8 | 1.3 | 1.7 | 1.6 | 1.3 | 1.6 | 1.6 | 1.0 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.14 | -0.34 | -0.34 | -0.3 | -0.51 | -0.54 | -0.68 | -0.61 | 0.01 | 0.08 | -0.01 | 0.18 | 0.27 | 0.19 | -0.2 | 0.11 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | 1.3 | 1.5 | 1.9 | 1.2 | 2.0 | 1.3 | 1.8 | 1.3 | 3.8 | 5.8 | 7.3 | 1.5 | 1.7 | 1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.3 | 0 | 7.7 | 10.4 | 6.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 1.7 | 0 |
Nucleus | -1.2 | -3.5 | -4.1 | -6.9 | -4.1 | -7.8 | -6.8 | -9.4 | -7.9 | -1.9 | -5.3 | -6.3 | -3.5 | -4.1 | -4.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0.4 | 1.1 | -0.9 | 0.1 | 0.4 | 0 | 0 | 0 | 0 | -0.2 | -1.9 | -1.8 | -1.5 | -2.4 | -2.2 |
Vacuole | -1.6 | 0.6 | 0 | -0.2 | -1.1 | 0.8 | 0.4 | -0.7 | 1.0 | 0 | 0.2 | -0.3 | 1.2 | 3.0 | 4.1 |