Standard name
Description Proliferating cell nuclear antigen (PCNA); functions as the sliding replication clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair; PCNA ubiquitination at K164 plays a crucial role during Okazaki fragment processing

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.9 0.96 0.99 0.98 0.91 0.92 0.94 0.72 0.84 0.7 0.7 0.97 0.99 0.82 0.73 0.79 0.88 0.83 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.07 0 0 0 0.06 0 0 0.31 0.18 0.39 0.33 0 0 0.11 0.11 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 0
Bud 1 2 0 2 1 0 0 0 8 2 3 9 0 0 0 1 0 0 1 1
Bud Neck 0 2 6 7 7 5 10 21 8 12 4 12 0 0 0 2 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Cell Periphery 0 0 2 0 0 3 1 2 0 3 2 2 1 0 0 1 2 0 0 0
Cytoplasm 55 140 114 237 328 379 380 531 221 285 235 282 88 78 72 115 99 15 64 49
Endoplasmic Reticulum 1 1 2 0 0 1 0 0 0 0 2 1 1 0 0 0 1 0 0 1
Endosome 0 1 1 0 0 6 4 1 2 2 3 2 0 0 1 0 0 0 0 1
Golgi 0 0 1 0 0 0 1 0 0 0 0 0 0 2 0 1 0 0 0 2
Mitochondria 8 11 1 1 4 26 18 25 96 60 129 133 0 0 10 17 12 0 0 1
Nucleus 0 2 0 1 0 1 2 4 1 5 2 3 0 0 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 1
Peroxisomes 0 1 0 0 0 2 1 1 0 0 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 3 5 13 15 11 9 7 19 0 0 0 0 1 0 0 1
Vac/Vac Membrane 1 3 0 0 2 12 7 4 3 6 4 2 1 0 1 7 0 1 1 0
Unique Cell Count 62 155 119 239 336 418 413 567 306 341 334 403 91 79 89 157 127 18 77 68
Labelled Cell Count 66 164 127 249 345 440 437 605 350 387 396 465 91 80 89 157 127 18 77 68


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 3.9 4.9 4.4 5.3 4.0 4.2 4.2 3.1 3.6 3.4 3.3 5.0 5.5 3.8 4.2 4.5 5.8 6.4
Std Deviation (1e-4) 0.8 3.4 1.9 1.5 2.4 1.4 2.0 1.6 0.8 1.4 1.3 0.8 2.0 3.2 1.5 0.7 1.1 2.6 3.1
Intensity Change (Log2) -0.15 0.11 -0.29 -0.22 -0.21 -0.64 -0.43 -0.53 -0.56 0.03 0.18 -0.36 -0.23 -0.12 0.24 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pol30-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available