ORF
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.91 0.7 0.47 0.48 0.32 0.26 0.27 0.21 0.21 0.16 0.94 0.97 0.97 0.85 0.85 0.83 0.68 0.46 0.68 0.63 0.66 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.13 0.1 0.17 0.19 0.14 0.15 0.12 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0.05 0.1 0.17 0.22 0.22 0.33 0.29 0.35 0.32 0 0 0 0.14 0 0.05 0.26 0.44 0.22 0.31 0.21 0.11
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.15 0.36 0.44 0.38 0.41 0.42 0.36 0.37 0.38 0.39 0.07 0.07 0.06 0.2 0.24 0.31 0 0.05 0 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 2 3 2 6 10 0 0 0 0 0 0 0 0 1 0 0 1
Bud 0 1 0 0 0 0 3 0 0 3 5 0 0 0 0 0 0 0 0 2 2 18 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1
Bud Site 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Cell Periphery 0 1 0 1 2 2 1 0 3 1 1 0 1 1 1 0 1 0 0 0 0 0 0
Cytoplasm 23 253 186 164 187 177 186 119 95 86 101 246 583 630 88 190 147 46 14 189 135 358 409
Endoplasmic Reticulum 0 4 3 6 6 5 10 4 7 1 2 5 1 3 3 8 12 0 0 1 0 1 7
Endosome 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0 1 0 3 5
Golgi 0 1 2 46 38 97 136 64 67 50 82 0 1 2 1 8 5 0 0 0 0 0 0
Mitochondria 0 0 0 1 0 1 0 5 5 4 3 1 0 0 0 1 0 0 0 0 0 0 0
Nucleus 0 3 14 36 68 121 161 150 135 145 200 1 0 0 15 7 8 18 13 60 65 117 60
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 1 6 8
Nucleolus 0 0 1 0 1 3 29 7 10 9 31 0 0 0 0 0 0 0 0 0 0 1 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 5
Vac/Vac Membrane 1 42 95 154 148 227 301 160 172 158 238 17 42 39 21 53 54 2 1 9 7 29 28
Unique Cell Count 24 279 264 350 389 556 723 448 461 417 617 261 602 652 104 224 177 69 31 280 215 545 544
Labelled Cell Count 24 305 301 408 451 633 829 514 497 463 674 270 628 675 129 267 228 69 31 280 215 545 544


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 28.7 30.8 29.0 34.7 29.9 29.0 27.2 23.9 22.4 21.5 21.7 37.0 39.0 37.5 30.9 36.4 40.6 33.8 35.0 34.9
Std Deviation (1e-4) 5.2 7.0 9.8 7.1 6.3 5.8 5.6 5.3 4.7 5.1 4.6 8.3 8.6 8.2 10.4 12.6 13.2 6.3 7.4 8.0
Intensity Change (Log2) -0.08 0.17 -0.04 -0.09 -0.18 -0.37 -0.46 -0.52 -0.51 0.26 0.34 0.29 0.0 0.24 0.4 0.13 0.18 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -6.0 -11.6 -11.5 -16.0 -18.6 -16.8 -18.5 -18.1 -21.1 1.6 3.9 3.8 -1.7 -2.0 -2.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1.6 3.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 6.1 5.1 7.2 7.6 6.4 6.5 5.8 6.2 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 2.8 4.8 6.8 7.9 8.1 10.4 9.5 10.6 10.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 3.4 0 0 0 3.8 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 5.6 7.8 6.5 7.5 7.9 6.0 6.5 6.5 7.0 -3.2 -3.8 -4.5 1.2 2.5 3.9


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ybr090c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available