ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | – | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 2 | 6 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud | 0 | – | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 18 | 9 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Bud Site | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | – | 1 | 0 | 1 | 2 | 2 | 1 | 0 | 3 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 23 | – | 253 | 186 | 164 | 187 | 177 | 186 | 119 | 95 | 86 | 101 | 246 | 583 | 630 | 88 | 190 | 147 | 46 | 14 | 189 | 135 | 358 | 409 |
Endoplasmic Reticulum | 0 | – | 4 | 3 | 6 | 6 | 5 | 10 | 4 | 7 | 1 | 2 | 5 | 1 | 3 | 3 | 8 | 12 | 0 | 0 | 1 | 0 | 1 | 7 |
Endosome | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 5 |
Golgi | 0 | – | 1 | 2 | 46 | 38 | 97 | 136 | 64 | 67 | 50 | 82 | 0 | 1 | 2 | 1 | 8 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | – | 0 | 0 | 1 | 0 | 1 | 0 | 5 | 5 | 4 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | – | 3 | 14 | 36 | 68 | 121 | 161 | 150 | 135 | 145 | 200 | 1 | 0 | 0 | 15 | 7 | 8 | 18 | 13 | 60 | 65 | 117 | 60 |
Nuclear Periphery | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 6 | 8 |
Nucleolus | 0 | – | 0 | 1 | 0 | 1 | 3 | 29 | 7 | 10 | 9 | 31 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Peroxisomes | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 5 |
Vac/Vac Membrane | 1 | – | 42 | 95 | 154 | 148 | 227 | 301 | 160 | 172 | 158 | 238 | 17 | 42 | 39 | 21 | 53 | 54 | 2 | 1 | 9 | 7 | 29 | 28 |
Unique Cell Count | 24 | 279 | 264 | 350 | 389 | 556 | 723 | 448 | 461 | 417 | 617 | 261 | 602 | 652 | 104 | 224 | 177 | 69 | 31 | 280 | 215 | 545 | 544 | |
Labelled Cell Count | 24 | 305 | 301 | 408 | 451 | 633 | 829 | 514 | 497 | 463 | 674 | 270 | 628 | 675 | 129 | 267 | 228 | 69 | 31 | 280 | 215 | 545 | 544 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 28.7 | – | 30.8 | 29.0 | 34.7 | 29.9 | 29.0 | 27.2 | 23.9 | 22.4 | 21.5 | 21.7 | 37.0 | 39.0 | 37.5 | 30.9 | 36.4 | 40.6 | 33.8 | 35.0 | 34.9 |
Std Deviation (1e-4) | 5.2 | – | 7.0 | 9.8 | 7.1 | 6.3 | 5.8 | 5.6 | 5.3 | 4.7 | 5.1 | 4.6 | 8.3 | 8.6 | 8.2 | 10.4 | 12.6 | 13.2 | 6.3 | 7.4 | 8.0 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.17 | -0.04 | -0.09 | -0.18 | -0.37 | -0.46 | -0.52 | -0.51 | 0.26 | 0.34 | 0.29 | 0.0 | 0.24 | 0.4 | 0.13 | 0.18 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -6.0 | -11.6 | -11.5 | -16.0 | -18.6 | -16.8 | -18.5 | -18.1 | -21.1 | 1.6 | 3.9 | 3.8 | -1.7 | -2.0 | -2.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 3.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 6.1 | 5.1 | 7.2 | 7.6 | 6.4 | 6.5 | 5.8 | 6.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 2.8 | 4.8 | 6.8 | 7.9 | 8.1 | 10.4 | 9.5 | 10.6 | 10.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 5.6 | 7.8 | 6.5 | 7.5 | 7.9 | 6.0 | 6.5 | 6.5 | 7.0 | -3.2 | -3.8 | -4.5 | 1.2 | 2.5 | 3.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 35.6276 | 39.2815 | 36.3691 | 36.646 | 32.8206 | 36.64 | 27.027 | 37.0188 | 33.3663 | 30.3439 | 29.4166 | 32.915 | 34.7988 | 41.503 | 36.5496 | 35.7199 | 37.1008 | 39.1445 |
Actin | 0.0053 | 0.0001 | 0.0052 | 0.0003 | 0.0004 | 0.0001 | 0.01 | 0.0007 | 0.0132 | 0.0004 | 0.0126 | 0.0002 | 0.0005 | 0.0001 | 0.0055 | 0.002 | 0.0001 | 0.0002 |
Bud | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0006 | 0 | 0.0008 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0008 | 0.0006 | 0.0007 | 0.0003 | 0.0004 | 0.0002 | 0.0012 | 0.0007 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0006 |
Bud Periphery | 0.0001 | 0 | 0.0003 | 0 | 0.0008 | 0 | 0.0009 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0002 | 0 | 0.0011 | 0 | 0.0003 | 0 | 0.003 | 0.0003 | 0.0016 | 0.0001 | 0.0024 | 0 | 0.0003 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.1587 | 0.3296 | 0.1617 | 0.1762 | 0.2269 | 0.2531 | 0.2194 | 0.3924 | 0.2533 | 0.2763 | 0.219 | 0.3877 | 0.288 | 0.4628 | 0.3023 | 0.3734 | 0.4313 | 0.4374 |
Cytoplasmic Foci | 0.0045 | 0 | 0.0002 | 0.0003 | 0.0015 | 0.0001 | 0.0355 | 0.0008 | 0.008 | 0.0048 | 0.0267 | 0.0004 | 0.0066 | 0.0001 | 0.004 | 0.0018 | 0 | 0.0001 |
Eisosomes | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0007 | 0.0004 | 0.0006 | 0.0002 | 0.0016 | 0.0012 | 0.0092 | 0.0007 | 0.002 | 0.0011 | 0.0025 | 0.0011 | 0.0008 | 0.0002 | 0.0008 | 0.0001 | 0.0002 | 0.0005 |
Endosome | 0.0015 | 0 | 0.0014 | 0.0001 | 0.0009 | 0.0002 | 0.0348 | 0.0008 | 0.0075 | 0.0208 | 0.0266 | 0.0007 | 0.0053 | 0 | 0.0012 | 0.0005 | 0 | 0.0001 |
Golgi | 0.0007 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.006 | 0.0005 | 0.0017 | 0.0035 | 0.0299 | 0 | 0.0007 | 0 | 0.0005 | 0.0018 | 0 | 0 |
Lipid Particles | 0.0033 | 0 | 0.0002 | 0 | 0.0013 | 0 | 0.0225 | 0.0005 | 0.0013 | 0.0042 | 0.0245 | 0 | 0.0067 | 0 | 0.0032 | 0.0111 | 0 | 0 |
Mitochondria | 0.0072 | 0.0001 | 0.0004 | 0.0001 | 0.019 | 0.0001 | 0.0064 | 0.0123 | 0.0009 | 0.0102 | 0.0111 | 0.0003 | 0.0008 | 0.0002 | 0.002 | 0.0015 | 0.0001 | 0.0002 |
None | 0.0007 | 0.0001 | 0.0015 | 0.0001 | 0.0014 | 0.0001 | 0.025 | 0.0005 | 0.0094 | 0.0003 | 0.0017 | 0.0002 | 0.0013 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0548 | 0.0329 | 0.0406 | 0.0417 | 0.0486 | 0.0529 | 0.0411 | 0.0278 | 0.0338 | 0.0258 | 0.0222 | 0.0375 | 0.0445 | 0.0251 | 0.0269 | 0.0284 | 0.0216 | 0.0329 |
Nucleolus | 0.0031 | 0.0003 | 0.0009 | 0.0004 | 0.012 | 0.0002 | 0.0015 | 0.0006 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0002 |
Nucleus | 0.7491 | 0.6346 | 0.7832 | 0.7777 | 0.6697 | 0.689 | 0.5609 | 0.5559 | 0.6599 | 0.6462 | 0.6029 | 0.5688 | 0.6363 | 0.51 | 0.6469 | 0.5587 | 0.5454 | 0.5259 |
Peroxisomes | 0.0019 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0045 | 0.0002 | 0.001 | 0.0006 | 0.0112 | 0 | 0.0022 | 0 | 0.0008 | 0.0184 | 0 | 0 |
Punctate Nuclear | 0.0024 | 0.0006 | 0.0007 | 0.0012 | 0.0012 | 0.0006 | 0.0071 | 0.0043 | 0.0018 | 0.0011 | 0.0022 | 0.001 | 0.0035 | 0.0007 | 0.0018 | 0.0013 | 0.0006 | 0.0009 |
Vacuole | 0.0028 | 0.0007 | 0.0007 | 0.0007 | 0.0065 | 0.0014 | 0.0076 | 0.001 | 0.0024 | 0.0022 | 0.0013 | 0.0006 | 0.0009 | 0.0002 | 0.0012 | 0.0002 | 0.0002 | 0.0005 |
Vacuole Periphery | 0.0025 | 0.0002 | 0.0004 | 0.0004 | 0.0052 | 0.0004 | 0.002 | 0.0003 | 0.0007 | 0.0016 | 0.0007 | 0.0005 | 0.0005 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 62.7954 | 53.4621 | 39.6712 | 59.1026 | 67.8475 | 71.7264 | 72.9749 | 72.0555 | 70.4637 | 57.7086 |
Translational Efficiency | 1.9209 | 2.3669 | 2.7285 | 2.3798 | 1.5761 | 2.3054 | 1.5916 | 1.948 | 2.06 | 1.982 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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